| Code | CSB-E12108m |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 86-99 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 86 | ||||
| Range % | 80-89 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 83-99 | |||||
| The recovery of mouse NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The mouse NF-κB ELISA Kit is engineered for accurate measurement of mouse NF-κB levels from samples including serum, plasma, or tissue homogenates. It uses the Sandwich-ELISA mechanism in combination with the enzyme-substrate chromogenic reaction to measure the NF-κB content in the sample. The color intensity is positively correlated with NF-κB content in the sample. This kit has been validated against standards of sensitivity, specificity, precision, linearity, recovery, and lot-to-lot consistency.
NF-κB is a central mediator of inflammation with multiple links to thrombotic processes. It is involved in the regulation of expression of various genes encoding cytokine, chemokines, adhesion molecules, and anti-microbial peptides, thus orchestrating both innate and adaptive immune responses. Additionally, NF-κB plays a crucial role in modulating the survival, activation, and differentiation of innate immune cells and inflammatory T cells. It targets inflammation not only by boosting the synthesis of inflammatory molecules but also by regulating cell proliferation, apoptosis, morphogenesis, and differentiation. Abnormal NF-κB activation leads to aberrant T cell activation, which is related to autoimmune and inflammatory responses.
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I want to ask about sample processing of CSB-E12108m, Mouse Nuclear factor-kappa B, NF-κB ELISA Kit. I have a serum sample but before using it for the ELISA test I will treat it at a temperature of 56℃ for 30min for heat deactivation, would it affect the cytokine which I want to detect? What will be the sensitivity if my samples are preheated?
Generally speaking, the high temperature would denature the protein and affect the detection result. The mentioned treatment with a temperature of 56℃ for 30min is usually used in the inactivation. There should be not much effect to do so. However, our ELISA kit does not require extra special steps or operations. As to the serum preparation, we suggest you follow the manual.
