Mouse lymphotactin,Lptn/LTN ELISA Kit

Instructions
Code CSB-E08714m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name chemokine (C motif) ligand 1
Alternative Names Xcl1 ELISA Kit; Lptn ELISA Kit; Ltn ELISA Kit; Scyc1 ELISA Kit; Lymphotactin ELISA Kit; C motif chemokine 1 ELISA Kit; Cytokine SCM-1 ELISA Kit; Lymphotaxin ELISA Kit; Small-inducible cytokine C1 ELISA Kit
Abbreviation XCL1
Uniprot No. P47993
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Lptn/LTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 82-91
1:2 Average % 101
Range % 98-105
1:4 Average % 87
Range % 84-91
1:8 Average % 100
Range % 95-105
Recovery
The recovery of mouse Lptn/LTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-94
EDTA plasma (n=4) 107 103-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
400 2.974 2.869 2.922 2.736
200 2.352 2.368 2.360 2.174
100 1.567 1.602 1.585 1.399
50 1.081 1.078 1.080 0.894
25 0.667 0.648 0.658 0.472
12.5 0.416 0.402 0.409 0.223
6.25 0.328 0.312 0.320 0.134
0 0.185 0.187 0.186  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.
Gene References into Functions
  1. study provides in vitro and in vivo evidence that the interaction between XCL1 and alpha9 integrin has an important role for autoimmune diseases PMID: 28550205
  2. The interaction between XCL1 and XCR1 plays a crucial role in the classical immunology response. PMID: 27387353
  3. Data show that mice lacking either XCL1 or XCR1 exhibit similar specific defects in intestinal T cell populations as XCR1-dendritic cells (DC)-deficient mice. Therefore it is possible that the XCR1-XCL1 axis itself is directly involved in intestinal T cell homeostasis, via a mechanism that might either involve interaction with XCR1+ DCs or be independent of this pathway. PMID: 27005831
  4. T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production PMID: 22528127
  5. XCL1-mediated accumulation of dendritic cells in the thymic medulla contributes to naturally occurring regulatory T cell development and is regulated by Aire. PMID: 21300913
  6. Data report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. PMID: 20508642
  7. The expression patterns of XCR1 and XCL1 were conserved in human and mice blood cells, including certain dendritic cell subsets. PMID: 20541533
  8. MIP-1alpha, MIP-1beta, RANTES, and ATAC/lymphotactin function together with IFN-gamma as type 1 cytokines. PMID: 11972057
  9. Adenovirus-mediated intratumoral Lptn gene transfer potentiates the antibody-targeted superantigen therapy of experimental melanoma. PMID: 12226740
  10. In a brain-targeted HIV-1 Tat transgenic model, Tat expression up-regulates XCL1 production not only in Tat-expressing murine astrocytes, but also in monocytes and macrophages/microglia. PMID: 14734774
  11. Lymphotactin gene-modified dendritoma induces T-cell proliferation and strong cytotoxic T lymphocytes reaction against allogenic hepatocellular carcinoma cells. PMID: 17990361
  12. Results describe the role of XCL1 during the course of the immune response to pulmonary M. tuberculosis infection. PMID: 19097989

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Subcellular Location Secreted
Protein Families Intercrine gamma family
Tissue Specificity Expressed in activated CD8(+) T cells. In the thymus, expressed by medullary thymic epithelial cells.
Database Links

KEGG: mmu:16963

STRING: 10090.ENSMUSP00000027860

UniGene: Mm.190

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