Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human XCR1 transfected murine BaF3 cells is less than 100 ng/ml, corresponding to a specific activity of >1.0x10⁴ IU/mg.

Target Names

Xcl1

Uniprot No.

P47993

Research Area

Immunology

Alternative Names

Xcl1; Lptn; Ltn; Scyc1; Lymphotactin; C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; Small-inducible cytokine C1

Species

Mus musculus (Mouse)

Source

E.Coli

Expression Region

22-114aa

Complete Sequence

VGTEVLEESS CVNLQTQRLP VQKIKTYIIW EGAMRAVIFV TKRGLKICAD PEAKWVKAAI KTVDGRASTR KNMAETVPTG AQRSTSTAIT LTG

Mol. Weight

10.1 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Lymphotactin (XCL1) is the sole C-class chemokine, uniquely possessing a single disulfide bond and lacking the typical paired cysteine motif, making it a critical tool for studying XCR1-dependent dendritic cell recruitment and cross-presentation pathways. This tag-free recombinant mouse XCL1, encompassing the full mature sequence (aa 22–114), demonstrates potent chemotactic activity with an ED50 below 100 ng/ml in XCR1-transfected BaF3 migration assays, corresponding to a specific activity exceeding 1.0 × 10⁴ IU/mg. The endotoxin level of less than 1.0 EU/μg effectively minimizes LPS-driven artifacts in sensitive immune cell assays, supporting use in chemotaxis/migration experiments, in vivo inflammation or tumor models requiring XCR1⁺ DC mobilization, and as a reference standard in chemokine detection assays. Purity exceeding 97% by SDS-PAGE, combined with this stringent endotoxin control, satisfies the criteria typical for signaling pathway studies examining MAPK or NF-κB activation downstream of XCR1 engagement.

Target Background

Function

Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.

Gene References into Functions

study provides in vitro and in vivo evidence that the interaction between XCL1 and alpha9 integrin has an important role for autoimmune diseases PMID: 28550205
The interaction between XCL1 and XCR1 plays a crucial role in the classical immunology response. PMID: 27387353
Data show that mice lacking either XCL1 or XCR1 exhibit similar specific defects in intestinal T cell populations as XCR1-dendritic cells (DC)-deficient mice. Therefore it is possible that the XCR1-XCL1 axis itself is directly involved in intestinal T cell homeostasis, via a mechanism that might either involve interaction with XCR1+ DCs or be independent of this pathway. PMID: 27005831
T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production PMID: 22528127
XCL1-mediated accumulation of dendritic cells in the thymic medulla contributes to naturally occurring regulatory T cell development and is regulated by Aire. PMID: 21300913
Data report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. PMID: 20508642
The expression patterns of XCR1 and XCL1 were conserved in human and mice blood cells, including certain dendritic cell subsets. PMID: 20541533
MIP-1alpha, MIP-1beta, RANTES, and ATAC/lymphotactin function together with IFN-gamma as type 1 cytokines. PMID: 11972057
Adenovirus-mediated intratumoral Lptn gene transfer potentiates the antibody-targeted superantigen therapy of experimental melanoma. PMID: 12226740
In a brain-targeted HIV-1 Tat transgenic model, Tat expression up-regulates XCL1 production not only in Tat-expressing murine astrocytes, but also in monocytes and macrophages/microglia. PMID: 14734774
Lymphotactin gene-modified dendritoma induces T-cell proliferation and strong cytotoxic T lymphocytes reaction against allogenic hepatocellular carcinoma cells. PMID: 17990361
Results describe the role of XCL1 during the course of the immune response to pulmonary M. tuberculosis infection. PMID: 19097989

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Subcellular Location

Secreted.

Protein Families

Intercrine gamma family

Tissue Specificity

Expressed in activated CD8(+) T cells. In the thymus, expressed by medullary thymic epithelial cells.

Database Links

KEGG: mmu:16963

STRING: 10090.ENSMUSP00000027860

UniGene: Mm.190

Code	CSB-AP001201MO
Abbreviation	Recombinant Mouse Xcl1 protein (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Mouse Lymphotactin protein (Xcl1) (Active)

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