Recombinant Mouse GRB2-related adapter protein (Grap)

Code CSB-YP875150MO
MSDS
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Source Yeast
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Code CSB-EP875150MO
MSDS
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Source E.coli
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Code CSB-EP875150MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875150MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP875150MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Grap
Uniprot No.
Alternative Names
Grap; GRB2-related adapter protein
Species
Mus musculus (Mouse)
Expression Region
1-217
Target Protein Sequence
MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGAEGFVPK NYIRVKPHPW YSGRISRQLA EETLMKRNHL GAFLIRESES SPGEFSVSVN YGDQVQHFKV LREASGKYFL WEEKFNSLNE LVDFYRTTTI AKRRQIFLCD EQPLIKPSRA CFAQAQFDFS AQDPSQLSLR RGDIVEVVER EDPHWWRGRA GGRLGFFPRS YVQPVHL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway. Plays a role in the inner ear and in hearing.
Gene References into Functions
  1. Grb2-related adaptor protein (GRAP) was up-regulated in kidney tubules from diabetic mice, indicating a potential novel role for GRAP in TGF-beta-induced tubule injury in diabetic kidney disease. PMID: 19836472
  2. Data suggest that Grap, unlike Grb2, acts as a negative regulator of TCR-stimulated intracellular signaling by downregulating signal relay through the Ras/Erk pathway. PMID: 11971956
  3. Results suggest that the GRAP gene might have a role in the pathogenesis of Sjogren's syndrome. PMID: 15174222
  4. FcgammaRIIB docking site for the SH2 domain-containing adapters Grb2 and Grap PMID: 15456754
Subcellular Location
Membrane; Peripheral membrane protein. Cell junction, synapse.
Protein Families
GRB2/sem-5/DRK family
Tissue Specificity
Expressed in inner ear, in neruonal fibers innervating cochlear and utricular auditory hair cells (at protein level).
Database Links
CUSABIO guaranteed quality
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