Rat Somatostatin,SS ELISA Kit

Code CSB-E08204r
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P60042
Lead Time 3-5 working days
Abbreviation SST
Target Name somatostatin
Alias SMST, somatostatin-14|somatostatin-28
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details The hormone somatostatin has active 14 aa and 28 aa forms that are produced by alternate cleavage of the single preproprotein encoded by this gene. Somatostatin is expressed throughout the body and inhibits the release of numerous secondary hormones by binding to high-affinity G-protein-coupled somatostatin receptors. This hormone is an important regulator of the endocrine system through its interactions with pituitary growth hormone, thyroid stimulating hormone, and most hormones of the gastrointestinal tract. Somatostatin also affects rates of neurotransmission in the central nervous system and proliferation of both normal and tumorigenic cells.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 94  
Range % 92-98  
1:2 Average % 97  
Range % 92-99  
1:4 Average % 92  
Range % 87-96  
1:8 Average % 89  
Range % 82-94  
Recovery
The recovery of rat SS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
EDTA plasma (n=4) 98 92-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.229 2.128 2.179 1.978  
50 1.638 1.557 1.598 1.397  
25 1.112 1.101 1.107 0.906  
12.5 0.784 0.773 0.779 0.578  
6.25 0.496 0.485 0.491 0.290  
3.12 0.394 0.405 0.400 0.199  
1.56 0.303 0.307 0.305 0.104  
0 0.200 0.201 0.201    
References
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Function Somatostatin inhibits the release of somatotropin.
Subcellular Location Secreted
Protein Families Somatostatin family
Database Links

KEGG: rno:24797

STRING: 10116.ENSRNOP00000002519

UniGene: Rn.34418

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