CD9 Monoclonal Antibody

Code CSB-MA004969A1m
Size US$350
Image
  • Western Blot
    Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, MCF-7 whole cell lysate
    All lanes CD9 antibody at 1:2000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 25 KDa
    Observed band size: 25 KDa
    Exposure time: 5min
  • Western Blot
    Positive WB detected in: A549 whole cell lysate at 20μg, 10μg, 5μg, 2.5μg whole cell lysate
    All lanes CD9 antibody at 1:2000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 25 KDa
    Observed band size: 25 KDa
    Exposure time: 5min
  • Western Blot
    Positive WB detected in: 20μg A549 whole cell lysate
    All lanes: CD9 antibody at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 25 KDa
    Observed band size: 25 KDa
    Exposure time: 5min
  • Western Blot
    Positive WB detected in: 1.Exosomes extracted from plasma
    2.Exosomes extracted from serum
    3.Exosomes extracted from Hela cells
    All lanes: CD9 antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 25 KDa
    Observed band size: 25 KDa
    Exposure time: 5min
  • IHC image of CSB-MA004969A1m diluted at 1:50 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
  • IHC image of CSB-MA004969A1m diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of Hela cells with CSB-MA004969A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Immunofluorescence staining of HepG2 cells with CSB-MA004969A1m at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
  • Overlay histogram showing A549 cells stained with CSB-MA004969A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • Overlay histogram showing Jurkat cells stained with CSB-MA004969A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1(1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • Overlay histogram showing PC-3 cells stained with CSB-MA004969A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • Overlay histogram showing U87 cells stained with CSB-MA004969A1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Description

The monoclonal CD9 antibody is secreted from the hybridoma formed by the fusion of mouse myeloma cells and splenocytes from mice immunized with the recombinant human CD9 antigen protein (112-195AA). It is purified from mouse ascites through protein G, and its purity reaches over 95%. This unconjugated CD9 monoclonal antibody is matched with the mouse IgG1 isotype. It is suitable for ELISA, WB, IHC, IF, and FC applications and can only detect the human CD9 protein.

CD9 is a tetraspanin expressed by all the major subsets of leukocytes and also highly expressed by endothelial cells. Through interactions with other tetraspanins as well as with different transmembrane and intracellular proteins, CD9 influences many cellular activities, including intracellular signaling transduction, proliferation, activation, survival, migration, invasion, adhesion, and diapedesis.

Full Product Name Mouse anti-Homo sapiens (Human) CD9 Monoclonal antibody
Uniprot No. P21926
Target Names CD9
Alternative Names CD9; MIC3; TSPAN29; GIG2; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9
Raised in Mouse
Species Reactivity Human
Immunogen Recombinant Human CD9 antigen protein (112-195AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 1E8H1
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
WB WB:1:1000-1:32000
IHC 1:50-1:200
IF 1:50-1:200
FC 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Acts as a receptor for PSG17. Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis.
Gene References into Functions
  1. assays. Results from the present study demonstrated that CD9 was highly expressed in the highly metastatic Hepatocellular carcinoma (HCC)cells and promoted HCC cell migration. This protein may be a novel target for regulating the invasive phenotype in HCC. PMID: 29749468
  2. The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. PMID: 29671763
  3. CD9 expression predicts some clinical characteristics and indicates an unfavorable prognosis in acute lymphoblastic leukemia patients. PMID: 29286918
  4. CD9-CD81 blockage reduces exosome-mediated HIV-1 entry. PMID: 29429034
  5. Exosomal markers CD63 and CD9 are elevated in pancreatic tumor tissues. PMID: 28609367
  6. CD9 expression could be a biomarker for poor prognosis in invasive breast carcinoma PMID: 28178752
  7. CD9 stabilizes gp130 by blocking its ubiquitin-dependent lysosomal degradation to promote the IL6-gp130-bone marrow X-linked non-receptor tyrosine kinase-STAT3 signaling for maintaining GSC selfrenewal and tumorigenic capacity. PMID: 27740621
  8. CD9 was highly expressed on extravillous trophoblast (EVT) at the boundary region of EVT invasion and intravascular EVT. CD9 expression on Swan71 cells was reduced under hypoxic conditions, while its expression was increased by co-culture with HUVEC. CD9 could attenuate EVT invasion under the influence of an oxygen environment and maternal endothelial cells, proposing CD9 as a potential regulator of human placentation. PMID: 27780531
  9. As for 18Lin(-), CD34(-) HSCs are characterized by low expression of the tetraspanin CD9, which promotes homing, and high expression of the peptidase CD26, which inhibits homing. PMID: 28687990
  10. The findings suggest that, in contrast with previous models, the ligand-binding site of integrin alphaVbeta3, binds to the constant region (helices A and B) of the EC2 domain of CD9, CD81, and CD151 antigens. PMID: 27993971
  11. Data suggest that CD9 should be further evaluated as a target for glioblastoma treatment. PMID: 26573230
  12. Collectively, using tetraspanin CD9 in tandem with E-cadherin as a biomarker in renal cell carcinoma will help to not only distinguish between types, but also predict the metastatic potential of RCC. PMID: 26855131
  13. Data indicate that CD9 is implicated in BCC invasiveness and metastases by cellular mechanisms that involve specific CD9+ plasma membrane protrusions of BCCs. PMID: 25762645
  14. CD9-enriched microdomains negatively regulate LPS-induced receptor formation by preventing CD14 from accumulating into lipid rafts. [Review] PMID: 26378766
  15. Results indicate that CD9 downregulation promoted pancreatic cancer cell proliferation and migration through at least in part, enhancing the cell surface expression of EGFR. PMID: 25955689
  16. CD9 expression is upregulated and its expression is correlated with tumor stage and lymph node metastasis in esophageal squamous cell carcinoma patients PMID: 26045817
  17. Although the current findings did not prove any hypothesis, the indispensable role of CD9 in fertilization process was not excluded and the precise role of CD9 remains unexplained. [review] PMID: 25536312
  18. CD9 plays a role in the dysmegakaryopoiesis that occurs in primary myelofibrosis. PMID: 25840601
  19. High CD9 is associated with B acute lymphoblastic leukemia. PMID: 26320102
  20. These results suggested that the mechanism underlying CD9-induced suppression of cell proliferation may involve the inhibition of phosphorylation of EGFR and the activity of PI3K/Akt and MAPK/Erk signaling pathways PMID: 25760022
  21. OY-TES-1 downregulation in liver cancer cells inhibits cell proliferation by upregulating CD and downregulating NANOG. PMID: 25673160
  22. Low levels of CD9 coincidental with a novel nonsense mutation in glycoprotein Ibbeta in a patient with Bernard-Soulier syndrome. PMID: 26275786
  23. The cysteine residues involved in the formation of the disulfide bridges in CD9 EC2 were all essential for inhibiting multinucleated giant cell formation but a conserved glycine residue in the tetraspanin-defining 'CCG' motif was not. PMID: 25551757
  24. alteration in CD9 expression was sufficient to profoundly disrupt cellular actin arrangement and endogenous cell contraction by interfering with RhoA signaling. PMID: 25184334
  25. The mechanism responsible for this negative regulation exerted by CD9 on LFA-1 adhesion does not involve changes in the affinity state of this integrin but seems to be related to alterations in its state of aggregation. PMID: 26003300
  26. The results demonstrate that hypoxia regulates CD9 expression and CD9-mediated keratinocyte migration via the p38/MAPK pathway. PMID: 25200404
  27. Report shows that breast cancer cells contain a nuclear CD9 pool and that the abrogation of CD9 expression results in multipolar mitoses and polynucleation. PMID: 25103498
  28. this study indicated that sialylation involved in the development of MDR of AML cells probably through ST3GAL5 or ST8SIA4 regulating the activity of PI3K/Akt signaling and the expression of P-gp and MRP1. PMID: 24531716
  29. switch from alphavbeta5 to alphavbeta6 integrin plays a key role in CD9-regulated cell migration and MMP-9 activation in keratinocytes PMID: 25265322
  30. High expression of CD9 was statistically associated with older patients PMID: 24553302
  31. CD9 and CD63 tetraspanins block HIV-1-induced cell-cell fusion at the transition from hemifusion to pore opening. PMID: 24608085
  32. Loss of CD9 expression is associated with enhancement of invasive potential of malignant mesothelioma. PMID: 24466195
  33. CD9 and CD151 support integrin-mediated signaling at the immunological synapse. PMID: 24723389
  34. Introduction of CD9 expression in Raji cells resulted in significantly increased cell proliferation and HDAC activity compared to mock transfected Raji cells. PMID: 24747564
  35. Heparin-binding epidermal growth factor and CD9 are likely implicated in processes that are highly relevant for MS lesion formation PMID: 24038577
  36. this study points to EGFR as a key mediator between CD9-mediated pro-MMP-9 release and cellular invasion of HT1080 cells. PMID: 24246676
  37. The second extracellular loop of CD9 was responsible for the upregulation of MMP-9 production. PMID: 23840773
  38. This is the first study of the expression and prognostic potential of the tetraspanins in oral dysplasia. PMID: 24201754
  39. Low CD9 expression is associated with malignant mesothelioma. PMID: 23128478
  40. Both CD9/CD81-silenced cells and CD151-silenced cells showed delayed alpha3beta1-dependent cell spreading on laminin-332. PMID: 23613949
  41. Data indicate that CD9 acts as scaffold and assembles a ternary JAM-A-CD9-alphavbeta3 integrin complex from which JAM-A is released upon bFGF stimulation. PMID: 23389628
  42. these data suggest that CD9 is a novel marker for a human germinal center-B cell subset that is committed to plasma cell lineage. PMID: 23291167
  43. CD9 overexpression was confirmed in osteotropic cells. CD9 was significantly overexpressed in bone metastases versus primary tumors and visceral metastatic lesions. PMID: 23225418
  44. tetraspanin CD9 modulates molecular organization of integrins in lymphatic endothelial cells, thereby supporting several functions required for lymphangiogenesis PMID: 23223239
  45. Low CD9 expression is associated with gallbladder neoplasms PMID: 22613496
  46. identifies human male germ cells with capability of long-term survival and cell turnover in the xenogeneic testis environment PMID: 22592495
  47. Knockdown of CD9 by siRNA and blockage of CD9 activity by ALB6 in ovarian cancer cells demonstrated that constitutive activation of NF-kappaB is CD9 dependent and that CD9 is involved in anti-apoptosis PMID: 22095071
  48. CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression. PMID: 19692500
  49. The absence or down-regulation of CD9 expression and point mutation may play a considerable role in the pathway of the malignant transformation in the BEAS-2B cells induced by mineral powder. PMID: 17997888
  50. CD9 associates with ADAM17 and, through this interaction, negatively regulates the sheddase activity of ADAM17. PMID: 21365281

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Subcellular Location Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, extracellular exosome.
Protein Families Tetraspanin (TM4SF) family
Tissue Specificity Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.
Database Links

HGNC: 1709

OMIM: 143030

KEGG: hsa:928

STRING: 9606.ENSP00000009180

UniGene: Hs.114286

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