FOS Antibody

Code CSB-RA008790A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate
    All lanes: c-FOS antibody at 0.81μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 41, 29, 37 KDa
    Observed band size: 62 KDa

  • IHC image of CSB-RA008790A0HU diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA008790A0HU diluted at 1:81 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-RA008790A0HU at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Overlay histogram showing Hela cells stained with CSB-RA008790A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

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Product Details

Uniprot No. P01100
Target Names FOS
Alternative Names Activator protein 1 antibody; AP 1 antibody; C FOS antibody; Cellular oncogene c fos antibody; Cellular oncogene fos antibody; FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody; FBJ murine osteosarcoma viral oncogene homolog antibody; FBJ murine osteosarcoma viral v fos oncogene homolog antibody; FBJ Osteosarcoma Virus antibody; FOS antibody; FOS protein antibody; FOS_HUMAN antibody; G0 G1 switch regulatory protein 7 antibody; G0/G1 switch regulatory protein 7 antibody; G0S7 antibody; Oncogene FOS antibody; p55 antibody; proto oncogene c Fos antibody; Proto oncogene protein c fos antibody; Proto-oncogene c-Fos antibody; v fos FBJ murine osteosarcoma viral oncogene homolog antibody
Species Reactivity Human, Mouse
Immunogen A synthesized peptide derived from human FOS
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Gene References into Functions
  1. Findings iindicate a human bone tumour defined by mutations of FOS and FOSB. PMID: 29858576
  2. gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
  3. Mutant cellular AP-1 proteins promote expression of a subset of Epstein-Barr virus late genes in the absence of lytic viral DNA replication. PMID: 30021895
  4. Low c-fos expression is associated with Oral Squamous Cell Carcinoma. PMID: 29582647
  5. Study demonstrated that c-Fos was highly expressed in most of ovarian epithelial carcinoma cases and was significantly correlated with Lewis y. Also, the results revealed that c-Fos interacted with the FUT1 promoter. Silencing of c-Fos prevented TGF-beta1-induced Lewis y expression. PMID: 29130097
  6. These findings indicate that the c-Fos/miR-22/MDC1 axis plays a relevant role in DNA repair in terminally differentiated cells, which may facilitate our understanding of molecular mechanism underlying the downregulating DNA repair in differentiated cells. PMID: 28637007
  7. our results strongly suggest a novel role of c-Fos as a regulator of epithelial-mesenchymal transition and cancer stem cell(CSC) reprogramming in Head and neck squamous cell carcinoma (HNSCC)cells, which may hold potential as a CSC-directed therapeutic approach to improve HNSCC treatment PMID: 27965308
  8. High c-fos expression is associated with malignant glioma. PMID: 27602752
  9. Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
  10. data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9 PMID: 28665975
  11. These findings demonstrate an essential role for the ERK pathway together with c-JUN and c-FOS in the differentiation activity of LukS-PV. PMID: 27102414
  12. novel function of KDM2B in the negative regulation of cell proliferation by assembling an E3 ligase to targeting c-Fos protein degradation that is antagonized by mitogenic stimulations PMID: 26725323
  13. NF-Y Binding Site Architecture Defines a C-Fos Targeted Promoter Class PMID: 27517874
  14. c-fos underexpression is associated with Myelodysplastic Syndrome. PMID: 27513856
  15. miR-101 is downregulated in bladder cancer cells and has an inhibitory role in the regulation of bladder cancer cell proliferation and invasion via directly targeting c-FOS. PMID: 27485165
  16. We found that c-jun or c-fos was significantly associated with lymph node metastasis, and coexpression of c-jun/c-fos, or c-jun/c-fos/p53 were significantly associated with lymph node metastasis, poor differentiation and clinical stage. PMID: 27558649
  17. CRAC channel blockade also suppressed Oxo-M-induced c-fos and interleukin-2 expression PMID: 27474128
  18. The results indicate that 17beta-estradiol-induced endometrial stromal cell invasion is dependent on c-fos-mediated MMP-9 expression. PMID: 26917263
  19. FOS is a downstream effector of high glucose stimulation in peritoneal mesothelial cells that contributes to TGF-beta1 production. PMID: 26018137
  20. VEGF-induced endothelial migration is mediated primarily by induction of JunB whereas the promotion of endothelial proliferation by VEGF is mediated by JunB-independent AP-1 family members. PMID: 26860974
  21. c-Fos can protect against HDAC3 neurotoxicity. PMID: 25592718
  22. These results indicate that IL-17A enhances COX2 expression and PGE2 production via the p38/c-Fos and JNK/c-Jun signalling pathways in NP cells to mediate intervertebral disc inflammation. PMID: 26988982
  23. the results of this study suggest that FOS is among the candidate genes of schizophrenia and that changes in the expression of c-Fos protein may contribute to molecular mechanisms of schizophrenia-related alterations in synaptic plasticity. PMID: 25706621
  24. Increased c-Fos expression is through TRPM3-mediated stimulation of the c-Fos promoter. PMID: 26493679
  25. A novel AP-1 binding site at -1363 bp of the human TF promoter region was identified. PMID: 26631725
  26. Simultaneous high expression of ID1 and c-Jun or c-Fos was correlated with poor survival in esophageal squamous cell carcinoma patients. PMID: 26858249
  27. miR-146a has a role in targeting Fos expression in human cardiac cells PMID: 26112171
  28. The translocation causes truncation of the FOS protein, with loss of the transactivation domain, which is thereby a novel mechanism involved in tumorigenesis. PMID: 26173738
  29. ERK1 and ERK2 regulated the expression of c-Fos and c-Jun proteins in human cervical cancer cells. PMID: 25647783
  30. O-GlcNAcylation of MLL5beta at T440 residue is critical for MLL5 recruitment to the HPV16/18-long control region through its interaction with AP-1. PMID: 25670814
  31. The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators. PMID: 26381409
  32. Data show that interleukin-1 receptor type 2 (IL1R2) forms a complex with c-Fos proto-oncogene protein and activates the interleukin-6 (IL-6) and vascular endothelial growth factor A (VEGF-A) promoters. PMID: 26209639
  33. Data indicate that deregulation of transcription factor AP-1 and microRNA-21-mediated axis led to an enhanced cell growth in hepatocellular carcinoma (HCC). PMID: 25544773
  34. These results establish c-Fos homodimers as a novel form of the AP-1 complex that may be an autonomous transcription factor in c-Fos-overexpressing tissues and could contribute to tumor development. PMID: 26303532
  35. Endoplasmic reticulum stress activates the hepatic AP-1 complex via MAPK-dependent signaling pathways. PMID: 25077945
  36. co-expression of c-Fos or Fra1 was able to cooperate with TAp73 in potentiating cellular growth, similarly to c-Jun. These data together suggest that TAp73 plays a vital role in activation of AP-1 target genes via direct binding to c-Jun PMID: 26018080
  37. The light-induced FOS response in melanopsin expressing HEK-293 cells is correlated with melanopsin quantity and dependent on light duration and irradiance. PMID: 24909488
  38. c-Fos promotes the progression of viral transcription from early to late stages and accelerates viral lytic replication upon sustained ORF45-ERK-RSK activation during the Kaposi's Sarcoma-Associated Herpesvirus lytic life cycle. PMID: 25903346
  39. By targeting the proto-oncogene Fos, miR-101 is involved in G1-to-S phase transition in cervical cancer cells in vitro. PMID: 24987920
  40. Data suggest that p38 MAP kinase regulates c-Fos/cellular oncogene fos mRNA stability/decay by affecting state of phosphorylation of ELAVL1/HuR (Hu antigen R). PMID: 25588078
  41. CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
  42. We found significant negative correlations regarding the expression of the genes COMT, MAOB, DRD4, DRD5 and FOS, indicating that increased schizotypy coincides with higher levels of dopaminergic dysregulation on the mRNA-level. PMID: 24630741
  43. results support the proposal that cooperative signaling of both NF-kappaB and AP1 (via p38alpha) amplifies STIM1 expression in ECs and, thereby, contributes to the lung vascular hyperpermeability response during sepsis PMID: 25016017
  44. SMAR1 has a role in repressing c-Fos-mediated HPV18 E6 transcription through alteration of chromatin histone deacetylation PMID: 25157104
  45. This study indicates that increased expression of c-Fos, p-c-Jun, members of AP-1 transcriptional factor and p-JNK is associated with neuronal degeneration in the ganglion cell layer of retinas in diabetic patients. PMID: 24073601
  46. S100A4, FOS and CXCR4, playing a major role in tumor progression and metastasis, are downregulated by sorafenib. PMID: 24378831
  47. the IL-1beta/p38/AP-1(c-fos)/MMP2 & MMP9 pathway play an important role in metastasis in gastric adenocarcinoma PMID: 24479681
  48. the distinct requirement of NF-kappaB for mouse and human c-fos regulation PMID: 24386331
  49. c-Fos, a well known AP-1 transcription factor, has emerged as a unique protein with the capacity to associate to specific enzymes of the pathway of synthesis of phospholipids at the endoplasmic reticulum and activate their synthesis. (Review) PMID: 24886961
  50. Inflammation mediators act through c-Fos to increase VEGF production in peritoneal mesothelium. PMID: 23760290
  51. C-myc, c-jun and c-fos expression values also showed a time-dependent increase in surviving patients. PMID: 23578467
  52. In the hippocampus and cerebellum, c-fos expression is decreased in Niemann-Pick type C knock-out mice. PMID: 23660496
  53. Sumoylation of c-fos could serve to buffer transcriptional actiSumoylation of the c-fos-c-jun complex could serve to buffer transcriptional activation of AP-1 target genes.vation of AP-1 target genes. PMID: 23396363
  54. c-FOS might influence ovarian cancer progression by changing the adhesion of ovarian cancer cells to peritoneal surfaces PMID: 24322891
  55. These findings indicate miR-490-5p to be a novel tumor suppressor of bladder cancer cell proliferation through targeting c-Fos. PMID: 24220339
  56. NF-Y performs a diverse set of biological functions, many of which are likely to involve coassociation with FOS. PMID: 23595228
  57. When the histone H3 nonphosphorylatable mutant of serine 10 (S10A) was overexpressed in cells, arsenite induction of FOS, EGR1 and IL8 expression was significantly decreased as compared with wild-type cells. PMID: 22354777
  58. Data indicate that estrogen induced the expression of c-Jun, Fra-1, c-Fos, cyclin D1 and cyclin E1 while tamoxifen (TMX) decreased the expression. PMID: 23625206
  59. Protein kinase R modulates c-Fos and c-Jun signaling to promote proliferation of hepatocellular carcinoma with hepatitis C virus infection. PMID: 23844083
  60. Diminished c-fos expression might play a role in congenital generalized lipodystrophy (CGL) by interfering with adipocyte development. PMID: 23919306
  61. The expression of FOS in glioma was increased and strongly correlated with its pathological grade. PMID: 23744317
  62. Down-regulation of c-fos by shRNA sensitizes adriamycin-resistant MCF-7/ADR cells to chemotherapeutic agents via P-glycoprotein inhibition and apoptosis augmentation. PMID: 23494858
  63. Derepression of c-Fos caused by miR-139 down-regulation contributes to the metastasis of hepatocellular carcinoma. PMID: 23001723
  64. This is the first report demonstrating attenuation of stress-induced FOS mRNA stabilization in myelodysplastic syndromes granulocytes. PMID: 23593403
  65. PRL-3 plays a critical role in ovarian cancer tumorigenicity and maintaining the malignant phenotype. PRL-3 may inhibit c-fos transcriptional regulation of integrin alpha2 signaling. PMID: 23418787
  66. HBXIP nuclear import requires interaction with c-Fos and phosphorylation of both proteins in breast cancer cells PMID: 23667255
  67. Fra-1 and c-Fos have roles in supporting growth of human malignant breast tumors by activating membrane biogenesis at the cytoplasm PMID: 23301044
  68. we identify a HOXB7/PBX2-->miR-221&222 -->c-FOS regulatory link, whereby the abrogation of functional HOXB7/PBX2 dimers leads to reduced miR-221&222 transcription and elevated c-FOS expression with consequent cell death PMID: 23400877
  69. gain-of-function mutant cellular c-Jun and c-Fos proteins preferentially bind methylated DNA, which disrupts Epstein-Barr virus latency PMID: 23625009
  70. cFos mediates cAMP-induced ROS generation, a critical mediator of neutrophil maturation PMID: 23209736
  71. Report c-Fos expression in skin keratinocytes/fibroblasts and keloid fibroblasts exposed to genistein. PMID: 23614275
  72. PAX2 protein induces expression of cyclin D1 through activating AP-1 protein and promotes proliferation of colon cancer cells. PMID: 23135283
  73. Novel cues reinstate responding to a similar extent as conditioned cues, regardless of whether animals have a reinforcement history with cocaine or sucrose; both types of cues activate similar brain circuits. PMID: 22534624
  74. bFGF induces the increased expression of c-fos through PI 3-K/PKB in CNE- I nasopharyngeal carcinoma cell line. PMID: 15835820
  75. There was a significant increase in the expression of the c-Fos gene in endothelial cells incubated with control plasma from 16 and 28 weeks of gestation, with no significant difference between the two time points with plasma from pre-eclampsia cases. PMID: 23121125
  76. The decrease in c-Fos methylation levels might be involved in the early phase of apoptosis induced by NaF in L-02 cells. PMID: 22528769
  77. These results suggest that EGF is secreted from A549 cells by MMP and increases claudin-2 expression mediated via the activation of an EGFR/MEK/ERK pathway. PMID: 22546605
  78. FOS/AP-1 inhibition in SCC cell lines induced p53 expression. Epidermal cell differentiation and skin tumor suppression were caused by a p53-dependent transcriptional activation of the metalloprotease TACE. PMID: 22772468
  79. Protein kinase C stimulates human B cell activating factor gene expression through reactive oxygen species-dependent c-Fos in THP-1 pro-monocytic cells PMID: 22537850
  80. c-Src is capable of phosphorylating tyr residues of c-Fos whereas the phosphatase TC45 T-cell protein-tyr phosphatase (TC-PTP) dephosphorylates them PMID: 22105363
  81. Variation in the expression of c-Fos leads to substantial diversity of IL-2 expression in approximately 40% of the memory Th cells PMID: 22474330
  82. Polypeptide from Chlamys farreri could attenuate UVA-induced Fas and c-fos expression, which may attribute to its protective effect against UVA-induced damage in HaCaT cells. PMID: 20726226
  83. Acrylamide can induce high protein expressions of c-fos and c-jun proto-oncogenes and apoptosis in L-02 cells. PMID: 20459032
  84. results suggest that, in addition to the indirect mechanisms that appear to mediate the antiproliferative properties of 1,25-(OH)2D3 in colorectal cancer cells, the hormone also exerts direct effects on both c-FOS and c-MYC PMID: 22108803
  85. an intricate network of energetically-coupled residues within the basic regions of the Jun-Fos heterodimeric transcription factor accounts for its allosteric binding to DNA PMID: 21496208
  86. Results suggest that IL-3 plays an inhibitory role in osteoclast differentiation by regulating c-Fos and Id1, and also exerts anti-bone erosion effects. PMID: 21732357
  87. c-Fos transcript levels in blood leukocytes could be considered as a cumulative biomarker of smoking in coronary artery disease. PMID: 21978922
  88. High C-fos is associated with rectal cancer. PMID: 21975339
  89. Data show that HPV16E6-dependent c-fos oncogenic protein expression contributes to AP-1 complex formation under oxidative stress in SiHa cells. PMID: 21822357
  90. c-Fos acts as a positive regulator of HCV propagation and may contribute to HCV-associated pathogenesis. PMID: 21920361
  91. AP-1-dependent up-regulation of the P2 promoter, SLE T cells fail to further increase their basal CREM levels upon T cell activation due to a decreased content of the AP-1 family member c-Fos PMID: 21757709
  92. Elevated expression of c-fos in CNS might be one of key mechanisms in etiology of irritable bowel syndrome. PMID: 21340718
  93. Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells PMID: 21655091
  94. PPAR-delta agonists inhibit TGFalpha-induced MMP9 expression in human keratinocytes. This inhibition involves repression of c-fos binding at the MMP9 promoter. PMID: 21496113
  95. CEBPbeta, CYP1B1, and FOS mRNA and protein expression was increased in Pulmonary Artery Smooth Muscle Cellss derived from pulmonary arterial hypertension patients. PMID: 21303932
  96. silencing of c-Fos expression by miR155 is a conserved process that is required for dendritic cell maturation and function PMID: 21385848
  97. The c-fos and c-myc expression was remarkably downregulated in nasal polyps from topical steroid treated patients compared to untreated patients. PMID: 18422165
  98. results demonstrating dispensability of enhancer AP-1 binding motifs in infectious life cycle of cytomegalovirus and its compensation through NF-kappaB elements evidence functional robustness of major immediate-early enhancer regulation by host factors PMID: 21106746
  99. A novel role of the surface protease GP63 in the Leishmania-mediated subversion of host AP-1 activity. PMID: 20976196
  100. FOS mRNA levels appear to be a sensitive marker of statin treatment that is dissociated from cholesterol levels PMID: 20619839

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Subcellular Location Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol
Protein Families BZIP family, Fos subfamily
Database Links

HGNC: 3796

OMIM: 164810

KEGG: hsa:2353

STRING: 9606.ENSP00000306245

UniGene: Hs.25647

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