PAM Antibody

Datasheet

Code	CSB-PA017417LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate All lanes: PAM antibody at 5.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 97, 101, 99, 107 kDa Observed band size: 109 kDa IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA017417LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA017417LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PAM Polyclonal antibody

Uniprot No.

P19021

Target Names

PAM

Alternative Names

AMD_HUMAN antibody; PAL antibody; PAM antibody; Pancreatic peptidylglycine alpha amidating monooxygenase antibody; Peptidyl alpha amidating enzyme antibody; Peptidyl alpha hydroxyglycine alpha amidating lyase antibody; Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody; Peptidylamidoglycolate lyase antibody; Peptidylglycine 2 hydroxylase antibody; Peptidylglycine alpha amidating monooxygenase antibody; Peptidylglycine alpha hydroxylating monooxygenase antibody; PHM antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Peptidyl-glycine alpha-amidating monooxygenase protein (338-497AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PAM Antibody (Product code: CSB-PA017417LA01HU) is Non-conjugated. For PAM Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017417LB01HU	PAM Antibody, HRP conjugated	ELISA
FITC	CSB-PA017417LC01HU	PAM Antibody, FITC conjugated
Biotin	CSB-PA017417LD01HU	PAM Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Bifunctional enzyme that catalyzes the post-translational modification of inactive peptidylglycine precursors to the corresponding bioactive alpha-amidated peptides, a terminal modification in biosynthesis of many neural and endocrine peptides. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate. The second step, catalyzed by the peptidylglycine amidoglycolate lyase (PAL) domain, is the zinc-dependent cleavage of the N-C-alpha bond, producing the alpha-amidated peptide and glyoxylate. Similarly, catalyzes the two-step conversion of an N-fatty acylglycine to a primary fatty acid amide and glyoxylate.

Gene References into Functions

PAM single nucleotide polymorphism rs13175330 is associated with hypertension and insulin resistance in a Korean population. PMID: 29162152
The ancient ability of PAM to localize to ciliary membranes, which release bioactive ectosomes, may be related to its ability to accumulate in intralumenal vesicles and exosomes PMID: 28377049
Data suggest that His108 and a substrate molecule are involved in the reductive pathway while His172 and Tyr79 are important in the catalytic pathway in the copper-centered electron transfer catalyzed by peptidylglycine monooxygenase. PMID: 26982589
PAM expression is increased in the secretory pathway of differentiated neurons. PMID: 26879543
Oxygen Sensitivity of the Peptidylglycine alpha-Amidating Monooxygenase (PAM) in Neuroendocrine Cells PMID: 26296884
Two missense variants in PAM, encoding p.Asp563Gly (frequency of 4.98%) and p.Ser539Trp (frequency of 0.65%), confer moderately higher risk of type 2 diabetes (OR = 1.23, P = 3.9 x 10(-10) and OR = 1.47, P = 1.7 x 10(-5), respectively) PMID: 24464100
Detail the production of the catalytic core of human peptidylglycine alpha-hydroxylating monooxygenase (hPHMcc) in Escherichia coli possessing a N-terminal fusion to thioredoxin (Trx). PMID: 22554821
Data indicate that catalytic inactivation of PHM caused by pH changes is accompanied by structural change between two states of the protein involving strong Cu-S interaction that does not involve M314. PMID: 22080626
nuclear retention of PAM mRNA is lost upon expressing the La proteins that lack a conserved nuclear retention element, suggesting a direct association between PAM mRNA and La protein in vivo PMID: 16107699

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Subcellular Location

Cytoplasmic vesicle, secretory vesicle membrane; Single-pass membrane protein.; [Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted. Note=Secreted from secretory granules.; [Isoform 4]: Secreted. Note=Secreted from secretory granules.

Protein Families

Peptidyl-alpha-hydroxyglycine alpha-amidating lyase family; Copper type II ascorbate-dependent monooxygenase family

Database Links

HGNC: 8596

OMIM: 170270

KEGG: hsa:5066

STRING: 9606.ENSP00000306100

UniGene: Hs.369430

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