Rat Interleukin 6,IL-6 ELISA KIT

Code CSB-E04640r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name interleukin 6 (interferon, beta 2)
Alternative Names Il6 ELISA Kit; Il-6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation IL6
Uniprot No. P20607
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 pg/mL-20 pg/mL
Sensitivity 0.078 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Q&A and Customer Reviews


Seeking quotation for ELISA kits of IL-6. I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.

Thanks for your inquiry.
We have this kit available:
#CSB-E04640r,Rat Interleukin 6,IL-6 ELISA KIT
Usually this kit is used to test commmon rats, but it can also detect SD Rats.Pls follow your actual test result. I will send you the kit manual by email and ask the local distributor to give you a quote soon.
 Customer Reviews
  • Tested application: ELISA
    Test sample: Serum,Tissue homogenate: heart tissue homogenate
    Species: Rat
    Review: The standard was worked perfectly in my experiment but I could don get much difference in control and disease group animal serum samples.
  • Sample type: Serum
    Sample species: Rat
    Review: The values matched those in literature. The reproducibility of the test was awesome.

Target Data

Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions
  1. In summary, this study demonstrated that IL-6 enhances mitochondrial biogenesis and survival in astrocytes under experimental septic conditions. Additionally, IL-6 plays the pro-survival role through IL-6/AMPK signaling pathway. PMID: 29674256
  2. Aerobic exercise improves the lipid metabolism disorder and interferes with germ cell apoptosis by reducing interleukin-6 and interleukin-6 receptor expression in nutritional obesity rat model. PMID: 28776758
  3. These findings indicated that IL-6 might exert dual effects on tendon-derived stem cells in vitro: strongly enhancing their proliferation but inhibiting their tenogenic differentiation via the JAK/Stat3 pathway. PMID: 29547593
  4. in adjuvant arthritis fibroblast-like synoviocytes, MeCP2 mediates hypermethylation of PTCH1 gene and decreases the expression of PTCH1 protein, thus activating Hedgehog signaling pathway and increasing secretion of IL-6 and TNF-alpha. PMID: 28573530
  5. IL-6 expression increased in all groups compared to control. Lgr5 expression was up-regulated in cells exposed to a single stressor, but down-regulated when multiple stressors were administered. PMID: 29018960
  6. IL-6 synthesis and secretion were connected to acute peritoneal dialysis fluid exposure, and this response was triggered by TRPA1 receptors, possibly located to non-neuronal cells. PMID: 28698251
  7. The interleukin 6 (IL-6)-174 polymorphism was significantly associated with prostate cancer in Slovak patients. PMID: 29848725
  8. The present study demonstrated that IL-6 RNA interference may protect the lung from acute lung injury induced by intestinal ischemia/reperfusion, and that this protective role may be associated with upregulation of IL-10. PMID: 28713893
  9. In the present study, isolated rat peritoneal mesothelial cells were treated with TGF-beta1 and/or IL-6 which elicited mesenchymal transition (MMT) as determined by morphologic and biochemical techniques ..Partial characterization of relevant signaling pathways identified Erk1/2 activation, which was enhanced by combined TGF-b1/IL-6 administration, as a crucial necessary factor in the transition.. PMID: 26825317
  10. Comparative Modulation of Levels of Oxidative Stress in the Liver of Anti-Tuberculosis Drug Treated Wistar Rats by Vitamin B12, Beta-Carotene, and Spirulina fusiformis: Role of NF-kappaB, iNOS, IL-6, and IL-10.( PMID: 28387444
  11. An intracellular adrenomedullin system reduces IL-6 release via a NF-kB-mediated, cAMP-independent transcriptional mechanism in rat thymic epithelial cells. PMID: 27619517
  12. Crocin attenuate Tumor Necrosis Factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in streptozotocin-induced diabetic rat aorta/ PMID: 27529541
  13. Administration of Methylglyoxal led to depletion of antioxidant enzymes, induction of fibrosis (p < 0.001), up-regulated expression of RAGE (3.5 fold), TGF-beta (4.4 fold), SMAD2 (3.7 fold), SMAD3 (6.0 fold), IL-6 (4.3 fold) and TNF-alpha (5.5 fold) in the heart tissues compared to control rats. PMID: 28091942
  14. After being treated with calcitriol following hemorrhagic shock resulted in better survival rate, lower serum levels of TNF-alpha and IL-6, and lesser hepatic, renal, and pulmonary histo-pathologic scores of injury in rats. PMID: 27180201
  15. TNF-alpha, IL-6 and IL-10 expressions, responsible for disparity in action of curcumin against cisplatin-induced nephrotoxicity in rats. PMID: 28258441
  16. there may be dysregulation in IL-6 production from astrocytes. PMID: 27539920
  17. Local cryotherapy's local and systemic anti-inflammatory effects are mainly IL-6/IL-17A-driven and TNF-alpha independent in the adjuvant-induced arthritis model PMID: 28759646
  18. This study suggests direct stimulation of autophagy as a novel mechanism for IL-6-mediated protection of beta cells from stress-induced apoptosis. PMID: 28592636
  19. These data support prior findings suggesting that IL-6 mediates the leptin-sensitizing effects of amylin on VMN neurons and that the inherent leptin resistance of DIO rats can be effectively reversed at a cellular level by IL-6. PMID: 27534878
  20. Exercise combined with ultrasound attenuates neuropathic pain in rats and is associated with downregulation of IL-6 and TNF-alpha, and with upregulation of IL-10. PMID: 28319508
  21. Two-way ANOVA showed an interaction of prenatal low protein and postnatal high fat energy diet on IL-6 and IL-1beta transcription. PMID: 28141871
  22. Study provided preliminary evidence showing that high frequency vagal stimulation attenuates the expression of early onset pro-inflammatory cytokines IL-6 and TNFalpha in a neonatal rat model of airway infection. PMID: 27049312
  23. Overexpression of IL-6 is sufficient to induce myofibroblastic proliferation, differentiation, and fibrosis, probably via increased TGF-beta1-mediated MMP2/MMP3 signaling during ischemic myocardial remodeling monoculture or coculture models under hypoxic conditions. PMID: 27348628
  24. this paper shows that antidepressant rhodioloside decreased pro-inflammatory cytokine interleukin-6 PMID: 27214337
  25. Data show that cardiac arrest (CA) increased HIF-1alpha and pro-inflammatory cytokines (PICs) including IL-6 and TNF-alpha in the hippocampus CA1 region. PMID: 27383646
  26. the release of IL6 from MSCs was regulated via the TLR2/NFkappaB signalling pathway. Endogenous IL6 reduced apoptosis and protected OGDinjured PC12 cells when they were cocultured with MSCs. PMID: 27108485
  27. The levels of TNF-alpha and IL-6 and the intestinal wet/dry weight ratios were higher in the subarachnoid hemorrhage groups compared to the control group. PMID: 27993875
  28. low-level laser therapy (LLLT) administered following muscle injury modulates the mRNA expression of MyoD and myogenin. Moreover, the both forms of LLLT administration were able to modulate the mRNA expression of IL-6 during the muscle repair process. PMID: 26914683
  29. our results indicate that UC-MSCs can alleviate insulin resistance in part via production of IL-6 that elicits M2 polarization. Additionally, human obesity and insulin resistance were associated with increased pro-inflammatory ATMs infiltration. Thus, MSCs may be a new treatment for obesity-related insulin resistance and T2D concerning macrophage polarized effects PMID: 26523620
  30. Results suggest that activated-p38[alpha]-regulated IL-6 synthesis constitutes a neuroprotective mechanism of NaHS against oxygen-glucose deprivation/reoxygenation-induced neuronal injury in PC12 cells PMID: 26760196
  31. decrease in all groups after manipulation. Data show that the postoperative interleukin 6 (IL-6) levels were significantly increased in the conventional cecum resection (CCR) group compared to the laparoscopic cecum resection (LCR) group. PMID: 26846568
  32. Ang III, similar to Ang II, has pro-inflammatory effects since it induces STAT3 leading to an induction of IL-6 mRNA expression. PMID: 24961501
  33. the adenylyl cyclase/cAMP/PKA pathway upregulates IL-1beta-induced IL-6 synthesis through enhancement of the JAK2/STAT3 pathway in C6 glioma cells PMID: 26527061
  34. LPS significantly increased the supernatant TNF-alpha and IL-6 concentrations and reduced TH protein expression in PC12 cells, while silencing Nogo-A could block these effects PMID: 26583134
  35. Data show that expressions of vascular endothelial growth factor, interleukin 6, but not toll-like receptor 4 were modulated in everolimus-treated group. PMID: 26390417
  36. Data show that the ratio of peripheral blood CD4(+)/CD8(+) T lymphocytes and the levels of connexin-40 (Cx40), interleukins IL-2, IL-4 and IL-6 are significantly elevated in SH rats. PMID: 26927369
  37. Data show pioglitazone inhibits inflammatory reaction by up-regulating the level of peroxisome proliferator activated receptor gamma (PPARgamma) mRNA and down-regulating the levels of inflammatory cytokines TNF-alpha and IL-6 mRNAs in traumatic brain injury (TBI). PMID: 26927377
  38. Diabetic rats show an increase in the contractions of distal colon partly via the IL-6-IL-6 receptor pathway. PMID: 26191141
  39. REV-ERBalpha has a role in circadian oscillation in the expressions of Alas1, Ppargc1a, and Il6 genes in rat granulosa cells PMID: 26102301
  40. Data show that the negative regulation of cardiac fibroblasts (CFs) differentiation by qiliqiangxin (QL) through an interleukin-6(IL-6) transcriptional mechanism. PMID: 25752645
  41. IL-6 activates PI3K and PKCzeta signaling and determines cardiac differentiation in rat embryonic H9c2 cells PMID: 26205888
  42. Knockdown of IL-1Beta increased IL-6 expression in cerebral hypoxia-ischemia. PMID: 24965599
  43. Early increase of calpain-2 in L5-ventral root transection rats might be responsible for the induction of allodynia via up-regulating IL-6 in DRG neurons. PMID: 25150005
  44. HGF differentially regulates IL-6 production in hepatocytes (induces) versus immune cells (suppresses), signifying disparate regulation of the cell sources involved in IL-6 production is a biologically relevant mechanism. PMID: 24763697
  45. Neuromodulatory effects of interleukin-6 (IL-6) and corticotropin-releasing factor receptor (CRFR) 1 in visceral pain and stress-induced defecation in the WKY rat model of irritable bowel syndrome were investigated. PMID: 25260633
  46. Studied Sporothrix schenckii induction of release of tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-6 and activation of the extracellular signal-regulated kinase (ERK) signaling pathway in mast cells. PMID: 25262023
  47. S100beta-positive dendritic-like cells can sense an increase in extracellular protons via GPR68 and respond by the production of IL-6 in order to suppress the up-regulation of Pomc expression. PMID: 25129106
  48. Interleukin 6 (IL-6) levels rapidly increased within 24 h after SAH, reached a peak 24 h after SAH, and decreased slightly at 48 h. SOCS-3 was involved in cerebral vasospasm after subarachnoid hemorrhage via an IL-6- and IL-10-related mechanism. PMID: 24463741
  49. The neuronal apoptosis rate was positively correlated with the levels of TNF-alpha and IL-6, and negatively correlated with IL-10 and GDNF. PMID: 24706318
  50. The continuing increase in the production of IL-6 in culture was accompanied by a decreased formation of erythroblastic islets de novo. PMID: 25682689
  51. Data indicate that after loading jump (JL) for 6 weeks, the expressions of of interleukin 6 (IL-6), and ligand of receptor activator of NF-kappaB (RANKL) genes were markedly elevated in tibia. PMID: 25200151
  52. We found cardiac and histological changes compatible with the inflammatory process triggered by acute pancreatitis with the promotion of local myocardial cytokine production. PMID: 24865534
  53. These data, taken together, suggest that treatment with torin2 and rapamycin induces IL-6 secretion by astrocytes and may contribute to the reduction of mechanical hypersensitivity after SCI. PMID: 24667246
  54. Interleukin-6 contributes to the paracrine effects cardiosphere-derived cells. PMID: 24623002
  55. We demonstrated that IL-6 regulates the BTB by inhibiting the degradation of BTB constitutive proteins and activating ERK-MAPK pathways. PMID: 24584780
  56. We also show that there is a complex cross-talk between IL-6, BDNF, the Adenosine A1 and A2a receptors that results in neuroprotection of retinal ganglion cells. PMID: 24845382
  57. Exercise training intensity is an important factor determining cerebrospinal IL-6 concentration after exhaustive exercise. PMID: 24311225
  58. Hepatic expression of metallothionein I/II, glycoprotein 96, IL-6, and TGF- beta in rat strains contributed to different susceptibilities to experimental autoimmune encephalomyelitis. PMID: 24489578
  59. The mRNA expression of IL-6 was doubled in the intestinal mucosa of formula-fed vs. breast-fed rats. PMID: 24210203
  60. IL-6 could regulate prolactin by acting on pituitary vasoactive intestinal peptide PMID: 23700204
  61. Myocardium Caspase-3 mRNA, SOCS-1, SOCS-3, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) expression levels and Apoptosis index in myocardium ischemia-reperfusion rats, were examined. PMID: 24091940
  62. activated spinal p38 MAPK enzyme may play an important role in cellular IL-6 signaling pathways in hyperalgesia variation during different stages of arthritis inflammation PMID: 23503893
  63. Intravesical administration of hyaluronic acid decreases interleukin-6 levels, as well as the severity of bladder inflammation and voiding frequency in a rat model of chronic cystitis. PMID: 23379983
  64. IL-6 up-regulates FSH-induced LHR production by increasing mRNA transcription, and JAK/signal transducer and activator of transcription 3 signaling is required for up-regulation by IL-6 in granulosa cells PMID: 24467743
  65. IL-6 downregulation as a result of myogenic differentiation was associated with increased leucocyte-mediated cell death. PMID: 23802625
  66. regulation of pro-inflammatory cytokines expression in rat cardiovascular tissues by sympathetic nervous system and angiotensin II PMID: 23846262
  67. Olmesartan medoxomil completely reversed left ventricle hypertrophy and reduced IL-6 levels in renovascular hypertensive rats. PMID: 24379062
  68. Data suggest that water pollutants (cadmium, here) can up-regulate release of IL6 (interleukin-6) and TNF-alpha (tumor necrosis factor alpha) into blood in association with systemic oxidative stress. PMID: 23764358
  69. Inhibiting IL-6 in the orbitofrontal cortex impairs reversal learning. PMID: 24431453
  70. Investigated the effect of ischemic precondition to protect ischemia-reperfusion injury and reduce IL-6 expression in the rats liver transplantation. PMID: 23608380
  71. Under the combined effects of inflammation and orthodontic force, the expression of IL-6, IL-8 is increased. PMID: 23870461
  72. These results thus suggest that the expression of MMP-1 and MMP-3 in Tenon fibroblasts is regulated by IL-6 during, and may play an important role in, scar formation after glaucoma surgery PMID: 23086831
  73. Mcl-1L is an anti-apoptotic protein induced during liver regeneration after partial hepatectomy PMID: 23825534
  74. After treatment with 1 ng/ml IL-6, cell proliferation increased by 31%. PMID: 23869758
  75. To test the hypothesis that IL-6 contributes to mechanical allodynia, we treated SCIP rats with neutralizing IL-6 receptor antibody (IL-6-R Ab), and found that one systemic injection abolished allodynia and associated weight loss. PMID: 23639820
  76. IL-6 and STAT3 in the hypothalamus are activated in response to footshock and chronic unpredictable stress. Also, basal IL-6 signaling through the JAK/STAT3 pathway is required for the sustained CORT response to chronic, but not acute, cold stress PMID: 23218517
  77. Prolactin stimulates normal liver growth, promotes survival, and regulates liver regeneration by mechanisms that may include hepatic downregulation of IL-6 and upregulation of SOCS-3, increased hepatocyte proliferation, and angiogenesis. PMID: 23948778
  78. The results for IL-6 suggest that the neuroinflammatory reaction of dorsal root ganglia to nerve injury is propagated alongside the neuroaxis from the lumbar to the remote cervical segments PMID: 23634725
  79. high concentrations of free fatty acids increased the expression of P2X7 in PC12 cells which contributed to P2X7-mediated IL-6 release PMID: 23438872
  80. conclude that the IL-6/STAT1/STAT3 signaling pathway and its responsive genes after a single bout of resistance exercise are an important event regulating the SC pool and behavior involved in muscle hypertrophy PMID: 23451164
  81. inhibition of HIF-1/2alpha did not reverse the effects of hypoxia on IL-6 gene expression. In addition, phosphorylation of p38, but not JNK, was responsible for the effects of glucose deprivation on IL-6 gene expression. PMID: 23520526
  82. Data indicate that serum calprotectin levels correlated with serum levels of TNF-alpha and IL6 in TNBS colitis. PMID: 23685388
  83. Collectively, these findings showed that high glucose promoted apoptosis and cell growth arrest in podocytes via IL-6 signaling. PMID: 23692924
  84. Jmjd3-mediated H3K27me3 demethylation is crucial for IL-6 gene activation in endothelial cells PMID: 22578249
  85. Aerobic exercise training prevented the development of insulin resistance through IL-6 to a certain degree. PMID: 23294974
  86. These data comprise clear evidence that IL-6 is actively involved in the attempt of injured retinal ganglion cells to regenerate their axons. PMID: 22884876
  87. Shark cartilage polysaccharides reduce the secretion of IL-6 and IL-12 in rheumatoid arthritis. PMID: 22992031
  88. The attenuation of IL-6 secretion was associated with decreased phosphorylation of the stress-related p38 mitogen-activated protein kinase (p38 MAPK) and preserved insulin action. PMID: 22911046
  89. data indicate the possibility that interleukin 6 might be involved in the downregulation of occludin expression and in the modulation of blood-testis barrier permeability that occur in rats undergoing autoimmune orchitis PMID: 23018187
  90. The data of this stuidy suggested that the increased IL-6 in afferent neurons and spinal cord contribute to the development of neuropathic pain following motor fiber injury, and that TNF-alpha is responsible for the up-regulation of IL-6. PMID: 23261764
  91. IL-6, TNF-alpha, and iNOS is associated with decreased colonic contraction in rats with multiple organ dysfunction syndrome. PMID: 22480838
  92. Liver X receptor regulates rheumatoid arthritis fibroblast-like synoviocyte invasiveness, matrix metalloproteinase 2 activation, interleukin-6 and CXCL10. PMID: 22634718
  93. Xinfeng Capsule reduces expression of IL6, IL17 and platelet-activating factor, and reduces paw swelling of rats with adjuvant arthritis. PMID: 22152774
  94. Tumor necrosis factor (TNF)alpha may regulate IL-6 production through activation of janus kinase (JAK)/STAT signaling PMID: 22697788
  95. Down-regulation of the IL-17RA receptor by shRNA decreased IL-6 expression induced by IL-17A via p38 MAPK and ERK1/2 phosphorylation in HSCs PMID: 22851861
  96. IL6 exerts positive effects on beta-cell signaling, metabolism, antioxidant status, and insulin secretion. PMID: 22761278
  97. IL-6 plays a pivotal role in the interaction between degenerated discs and macrophages. PMID: 21494199
  98. Report antifibrotic activity of imatinib mesylate and suggest link between hepatic stem cell activation and enhanced Il-6 secretion. PMID: 22507133
  99. Prenatal surges in both maternal circulating IL-6 and leptin contribute to the appearance of sensitized dopaminergic neurons function in the adult offspring. PMID: 22133515
  100. Chronic endurance exercise could influence the inflammatory response of hepatic tissues, as well as muscle, and that the effects of chronic endurance exercise on inflammatory cytokine levels are different between old and young rats. PMID: 22554223

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Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: rno:24498

STRING: 10116.ENSRNOP00000013732

UniGene: Rn.9873

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