The rat interleukin 6 (IL6) ELISA kit is a valuable tool for researchers working with rat samples, offering a sensitive detection range from 0.312 pg/ml to 20 pg/ml with a remarkable sensitivity of 0.078 pg/ml. This kit is used to quantitatively detect IL6 in serum, plasma, and tissue homogenate from rats. The assay principle is based on a sandwich ELISA method, ensuring accurate and precise results. The assay can be completed within 1-5 hours, requiring a sample volume of 50-100 µl. The detection wavelength is set at 450 nm, providing reliable readings for IL6 levels in rat samples. Researchers can trust this kit to provide robust data on IL6 levels in rat biological samples, aiding in studies associated with inflammation and cytokine regulation. The kit is a bestseller because of its excellent parameters and has been cited in more than 325 articles.
In rats, IL6 responds to different stressors, including inflammatory and psychological stress. The expression of IL-6 in the rat brain has been linked to sustained hypothalamic inflammatory responses during fever induced by LPS [1]. Furthermore, IL-6 exerts anti-inflammatory effects through activating STAT3, thus protecting islets and β-cells from pro-inflammatory cytokine-induced apoptosis and loss of function [2]. IL-6 can activate target cells through classical and trans-signaling pathways [3][4]. Investigating IL-6's role in the central nervous system (CNS), a neuroprotective effect was observed when recombinant IL-6 was injected into the cerebral ventricles of rats [5].
References:
[1] J. Damm, G. Luheshi, R. Gerstberger, J. Roth, & C. Rummel, "Spatiotemporal nuclear factor interleukin‐6 expression in the rat brain during lipopolysaccharide‐induced fever is linked to sustained hypothalamic inflammatory target gene induction", The Journal of Comparative Neurology, vol. 519, no. 3, p. 480-505, 2010. https://doi.org/10.1002/cne.22529
[2] F. Lebreton, R. Hanna, C. Wassmer, K. Bellofatto, L. Perez, V. Othenin‐Girardet al., "Mechanisms of immunomodulation and cytoprotection conferred to pancreatic islet by human amniotic epithelial cells", Stem Cell Reviews and Reports, vol. 18, no. 1, p. 346-359, 2021. https://doi.org/10.1007/s12015-021-10269-w
[3] M. Conceição, L. Forcina, O. Wiklander, D. Gupta, J. Nordin, B. Vrellakuet al., "Engineered extracellular vesicle decoy receptor-mediated modulation of the il6 trans-signalling pathway in muscle", Biomaterials, vol. 266, p. 120435, 2021. https://doi.org/10.1016/j.biomaterials.2020.120435
[4] H. Ju, Y. Byun, J. Mok, & C. Joo, "The blockade of il6 counterparts the osmolar stress-induced apoptosis in human conjunctival epithelial cells", Journal of Ophthalmology, vol. 2016, p. 1-7, 2016. https://doi.org/10.1155/2016/8350134
[5] L. Ziegler, P. Frumento, H. Wallén, U. Faire, & B. Gigante, "The predictive role of interleukin 6 trans-signalling in middle-aged men and women at low-intermediate risk of cardiovascular events", European Journal of Preventive Cardiology, vol. 27, no. 2, p. 122-129, 2019. https://doi.org/10.1177/2047487319869694
