Code | CSB-RA024077A09phHU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
In the development of the phospho-TP53 (S9) recombinant monoclonal antibody, the initial phase involves the retrieval of genes encoding the TP53 antibody from rabbits immunized with a synthetic peptide originating from the human TP53 protein phosphorylated at S9. Following this, these genes are adeptly integrated into expression vectors. Subsequently, these genetically modified vectors are introduced into mammalian suspension cells, where they are thoughtfully cultivated to encourage the production and secretion of the antibodies. Following this growth phase, an intricate purification process employing affinity chromatography is executed to meticulously isolate the phospho-TP53 (S9) recombinant monoclonal antibody from the surrounding cell culture supernatant. Lastly, the functionality of the antibody is meticulously scrutinized through ELISA and IF tests, conclusively affirming its capacity to engage with the human TP53 protein phosphorylated at S9.
Phosphorylation of p53 at S9 serves as a crucial regulatory mechanism to maintain genomic integrity and prevent the development of cancer by coordinating DNA repair, cell cycle control, and cell fate decisions in response to stress and damage. Dysregulation of p53 phosphorylation can lead to uncontrolled cell proliferation and is often observed in cancer cells.
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