COVID-19 Antibody Reagents

Throughout the ongoing SARS-CoV-2 pandemic, researchers have been working tirelessly to understand the human immune response to SARS-CoV-2. Antibodies specific to diffident SARS-CoV-2 proteins are being used in COVID-19 research for vaccine development, drug discovery, diagnostics by the global scientists.

CUSABIO has developed a large collection of high quality antibody reagents to support research on COVID-19, including the N Antibody, S Antibody, S RBD Nanobody Antibody, N Antibody Pair, S RBD Antibody Pair. These antibodies can be used to detect and/or neutralize the virus in a variety of assays including ELISA, S-ELISA, western blot, Neutralising, GICA.

Partial Application Scenarios and Validated Data

● Qualitative Antigen Detection by WB

Positive WB detected in: His tag-tagged SARS-CoV-2 N recombinant protein (CSB-EP3325GMY); SARS-CoV-2 N antibody at 1:1000 (CSB-RA33255A1GMY)

Positive WB detected in: SARS-CoV-2 N recombinant protein (CSB-EP3325GMY) at 40ng, 20ng, 10ng; All lanes N antibody (CSB-MA33255A0m) at 1:1000

● Antigen-antibody Binding Activity Validated by Functional ELISA

Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/mL can bind SARS-CoV-2-N (CSB-RA33255A0GMY) Antibody, the EC₅₀ is 6.892 ng/mL.

Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-MA33245A0m), the EC₅₀ is 32.59 to 39.33 ng/mL.

● Used for Rapid Detection of Colloidal Gold Immunochromatography Assay (GICA)

In the GICA detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/mL (1.09ng/0.07mL), and the sensitivity is very good.

In the GICA detection system, the background of antibody (CSB-RA33245A0GMY) is clean, the detection limit can be as low as 13.94ng/mL (0.976ng/0.07mL), and the sensitivity is very good.

● Multiple Mutants can be Identified

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/mL can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC₅₀ of N Recombinant Antibody.

The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/mL can bind S Recombinant Antibody, the EC₅₀ of S Recombinant Antibody (CSB-RA33245A1GMY).