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| Code | CSB-YP001561BO |
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The recombinant Bovine Albumin (ALB) protein is a fusion protein consisting of the Bovine ALB protein (25-607aa) partnered with the C-6xHis-Myc Tag. It is a yeast-expressed full length of mature protein with a purity of 85%+ determined by SDS-PAGE. And it is featured with high purity and stability. You can use this Bovine Albumin protein for relevant bioassay and related research.
ALB is the gene encoding albumin, which is a kind of globular protein. For human types, albumin functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs.There are currently no reviews for this product.
Is the protein free of animal components?
We guarantee that all proteins produced are 100% animal-free, as we do not use any animal components in our raw materials and no animal components are added during the production process. Serum-free media is also used in the expression of our Mammalian proteins. If you need it, we can provide a declaration of no animal component.
Is it soluble?
All proteins that we expressed recombinantly are soluble proteins.
We're thinking about modifying the protein, so I need a construct that is expressed in bacteria preferably.
You could choose the E. coli expression system (product code: CSB-EP001561BO).
We need the rBSA (Recombinant Bovine Serum albumin) for large volumes of cell culture media. This media will be used on bovine cells and the final product is for human food NOT clinical or diagnostic use. Although, the culture vessels will be using anything from100-2000L medium with 0.5% rBSA. This means each run would need between 0.5kg and 10kg per vessel and several bioreactors would be needed for each run. Obviously, large quantities would only be needed once the process is perfected. Do you provide bigger quantities such as 5-50kg?
Yes, we have 50L, 500L and much larger fermenters, and have successfully prepared 50mg, 100mg, 200mg, 500mg and other large-scale proteins for many customers.
We have the ability to achieve large-scale protein production, from mg to g, even kg.I am preparing a research proposal (as part of a class to learn the theory) to study the efficacy of recombinant Bovine Serum Albumin (rBSA) compared to recombinant albumin that is derived from human genes for the purpose of bovine satellite cell culture. Can I get the quotation for 1mg and 1g protein? And I would also like to know if CUSABIO also sells recombinant albumin (from human genes), and if I could get a price comparison for that too.
For your experiment, do you need endotoxin removal and aseptic processing? We can provide it for free. Just put a note on your order so we will know.
As for rBSA, the quotation was already sent to you through email, you can order a small size to test first. If the test result is good then you can place bulk orders.
As for Recombinant Human Albumin, please check the product information listed below:
Uniport link: https://www.uniprot.org/uniprot/P02768
CSB-YP001561HU >> Yeast
CSB-EP001561HU >> E.coli
CSB-BP001561HU >> Baculovirus
CSB-MP001561HU >> Mammalian cell
Expression Region: 25-609aa; Full Length of Mature Protein
Tag information: Tag type will be determined during the manufacturing process.
Target Protein Sequence (The complete sequence will be provided upon request, including tag sequence, target protein sequence, and linker sequence):
DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL
I see the purity level of the product is 85%, is it good for cell culture usage? Also what is the other 15% what are the contaminants?
We guarantee a minimum purity standard of >85%. If the initial purification does not meet this standard or you have a higher purity requirement, we also have AKATA purification instrument, which is highly automated, precise control, combining the use of various columns, to ensure that the purity of our protein product is further enhanced and the final purity test results are displayed on the COA report.
The other 15% may be impurities such as nucleic acids, carbohydrates, lipids, unrelated proteins, isoenzymes, inactive proteins, etc.In theory, CSB-YP001561BO is suitable for cell culture. Generally, we recommend you to choose endotoxin removed and aseptic process services if you plan to use a protein in cell culture or cell-related experiments. It normally needs a high purity. Please remark your requirement for purity when you place the order.
How is the protein purified? Is the purity guaranteed?
We will design the optimal purification scheme according to the tag type of the fusion protein and the physicochemical properties of the protein itself.
Purification methods: affinity chromatography, hydrophobic chromatography, ion-exchange chromatography, molecular sieve, salting out, etc.We guarantee a minimum purity standard of >85%. The purity is comprehensively evaluated by SDS-PAGE Coomassie Brilliant Blue staining detection and Bandscan software analysis. Our QC standard of purity for all recombinant proteins is higher than 85% as determined by SDS-PAGE. We will decide if it needs a secondary AKTA-SEC purification by the SDS-PAGE result of the initial purity. If the initial purity is already qualified for the QC standard (>85%), and you don’t remark any strict requirement on the purity, generally we will not conduct AKTA-SEC for secondary purification. If the initial purification does not meet this standard or you have a higher purity requirement, we also have AKATA purification instrument, however, this AKTA-SEC purification requires an additional cost, and the delivery time will be extended by 3-5 working days.
Although we guarantee a minimum purity standard of >85%, some of the proteins we prepared can reach a purity of 95% or even 97%.I would like to know If you could highlight a few things about the bovine recombinant albumin produced in E. coli and Yeast:
What is the smallest packing size for this product?
How is the endotoxin level, and purity of each of the recombinant expression systems?
The smallest packing size for CSB-EP001561BO and CSB-YP001561BO is 20ug. And the other sizes such as 100ug, 500ug, 1mg are also available. We have the ability to achieve large-scale protein production, from mg to g, even kg.
We guarantee a minimum purity standard of >85% for each expression system, if you have a higher purity requirement, we can also try to meet your demand through AKTA-SEC purification, please communicate with us during the pre-sales inquiry.If you have a requirement for endotoxin level, please remark this information when placing the order and we could offer endotoxin removal service free of charge. We use conjugated PMB affinity filler adsorption to remove endotoxin and guarantee endotoxin level within 0.1ng/ug (1EU/ug). Generally, the delivery time will be extended for 2-3 days. Endotoxin removal result will be shown in COA as follows “<1.0 EU per 1μg of the protein by the LAL method.”
We noticed that this protein is C-terminal 6xHis-Myc-tagged, however, we don’t need any tag, can you provide this protein as tag-free?
If you need to remove the tag or tag-free protein, please communicate with us in advance, otherwise, we won't remove the tag. Usually, for most proteins with N-terminal tags, there is an enzyme cleavage site, and we can directly try to remove the tag by enzyme cleavage.
However, the tag of this protein is C-terminal 6xHis-Myc-tagged. It doesn't have any enzyme cleavage site. In such a case, if you need the tag-free version. We need to construct another new vector to express this protein and then try tag removal, the production cost will be much higher, please inquire for quotation.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we fail in removing the tag, we won’t charge for any extra cost, and remark this information in the datasheet as follows “Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Which validations have you performed for your proteins? Can you provide a free sample of this protein?
We carry out the following procedures, codon-optimized whole-gene synthesis → subcloning → construct expression plasmid → transformation and strain screening (small expression, optimization of expression conditions, determine the optimal expression conditions →scale-up expression→ protein purification→ If the expressed protein is inclusion body, it will adopt a variety of inclusion body renaturation to ensure the final supply of soluble protein→ QC test for purity, concentration, etc., and provide QC report. This series of production links will involve a variety of tests, 100% quality assurance:
(1) Sequencing → Ensure sequence correctness.
(2) Protein concentration detection.
(3) Molecular weight detection.
(4) Purity detection.
(5) Endotoxin removal will be performed upon request to ensure the final endotoxin level is less than 0.1 ng ug (1 EU/ug).
(6) Secondary activity test will be performed for Active Protein before the shipment.
(7) We will regularly perform Mass Spectrometry for mixed protein samples. If there are customers who need mass spectrometry results later, we can feedback the test results. For customers who require a separate mass spectrometry, we charge the customer for the appropriate cost and provide a single protein mass spectrometry.
(8) We can also carry out tag-antibody Western Blot for free. If the customer has this requirement, please remark this request when sending your PO and the test results will be shown in the COA report.
At present, our protein validation mainly includes SDS-PAGE and free tag-antibody WB verification.
In addition, if you have other verification requirements, you can always give us feedback. We can evaluate based on your needs and help you to perform further verification. Extra cost will be charged.
For some in-stock proteins, we could offer a small sample for free. If you need a free sample, please check with us during the pre-sales inquiry.
Why is the activity of this protein not guaranteed?
A: We have accumulated more than 15 years of experience in the expression of recombinant proteins and active proteins, and have produced more than 9000 recombinant proteins and more than 660 active proteins. A protein from the expression to the establishment of the activity test system to complete the activity verification requires a lot of cost and time investment.
This protein is not included in our activity verification plan currently. We are sorry that we have not tested it for a while and do not guarantee 100% activity. In theory, we are doing the full length of the protein, using affinity chromatography, and purifying the protein in a mild environment, which should be active in theory. We recommend that this customer can order a small size of this protein expressed by the eukaryotic expression system, which is more likely to be active.
(If our technical support can find relevant literature, the literature will be referred to customers.)
We will refer to the relevant literature on the preparation method of the active protein on the market, or if you have consulted the relevant literature and hope that we can prepare according to the methods in the literature, we will ensure the activity to the maximum according to the preparation methods mentioned in the literature.
Considering that the establishment of the protein measurement system requires a lot of time and cost to complete the measurement, although many of our proteins have not been verified by activity yet, many customers have detected very good activity and feedback to us after purchase, and even some customers have detected activity and successfully published literature.
I know activity isn’t measured – but in general –what is the impact of a given Tag type and any potential biological activity of the protein? Is a certain tag more or less likely to preserve biological activity – or does the Tag type not really make a difference? Or don’t you know?
Theoretically small tags generally have a very small influence on protein activity. However, the specific impact on protein activity can't be concluded (There is no impact on some proteins, small impact on some proteins, and relatively great impact on some proteins).
We should conduct specific analyses for specific proteins and specific tags. In the early stage, we could check related literatures. In the later stage, we will mainly validate by designing a control group.
We could design a parallel experiment for Fusion Protein & Tag-removed Protein or Fusion Protein & Tag-protein. We haven't done a relevant test for protein biological activity at present. So we can't 100% guarantee that the proteins have activity. (We will build up the detection of activity in a later stage)
