Code | CSB-MAP027691E0m |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
The BSA monoclonal antibody was produced through a hybridoma technology by immunizing mice with bovine serum albumin, isolating spleen cells to obtain antibody-producing B cells, and fusing them with myeloma cells. The hybridoma cells were then screened and cloned to select the cells that generated the BSA monoclonal antibody and then cultured in the mouse abdominal cavity. The resulting antibody was purified from the mouse ascites using protein G affinity chromatography to achieve a high level of purity (95%+). The BSA monoclonal antibody is specific to bovine BSA protein and can be used for ELISA and WB applications.
BSA is a protein commonly used as a standard in various biochemical applications, including enzyme assays, protein quantification, and electrophoresis. BSA is also used in cell culture media, diagnostic tests, and as a stabilizing agent for biomolecules.
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