Recombinant Mouse Myocilin(Myoc),partial

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Code CSB-BP015356MO
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names Myoc
Uniprot No. O70624
Research Area Epigenetics and Nuclear Signaling
Alternative Names Myoc; Tigr; Myocilin; Trabecular meshwork-induced glucocorticoid response protein) [Cleaved into: Myocilin; N-terminal fragment; Myocilin 20 kDa N-terminal fragment); Myocilin; C-terminal fragment; Myocilin 35 kDa N-terminal fragment)]
Species Mus musculus (Mouse)
Source Baculovirus
Expression Region 213-490aa
Target Protein Sequence ILKENPSGRPRSKEGDKGCGALVWVGEPVTLRTAETIAGKYGVWMRDPKPTHPYTQESTWRIDTVGTEIRQVFEYSQISQFEQGYPSKVHVLPRALESTGAVVYAGSLYFQGAESRTVVRYELDTETVKAEKEIPGAGYHGHFPYAWGGYTDIDLAVDESGLWVIYSTEEAKGAIVLSKLNPANLELERTWETNIRKQSVANAFVICGILYTVSSYSSAHATVNFAYDTKTGTSKTLTIPFTNRYKYSSMIDYNPLERKLFAWDNFNMVTYDIKLLEM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 35.2 kDa
Protein Length Partial
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. Modulates the organization of actin cytoskeleton by stimulating the formation of stress fibers through interactions with components of Wnt signaling pathways. Promotes cell migration through activation of PTK2 and the downstream phosphatidylinositol 3-kinase signaling (By similarity). Plays a role in bone formation and promotes osteoblast differentiation in a dose-dependent manner through mitogen-activated protein kinase signaling
Gene References into Functions
  1. mutant myocilin induces abnormal ECM accumulation in the ER of TM cells, which may be responsible for reduced outflow facility and IOP elevation in myocilin-associated glaucoma. PMID: 27820874
  2. Mutated myocilin and heterozygous Sod2 deficiency act synergistically in a mouse model of open-angle glaucoma PMID: 25740847
  3. myocilin promotes cell proliferation and resistance to apoptosis via the ERK1/2 MAPK signaling pathway. PMID: 24563482
  4. Myocilin binds to ErbB2/ErbB3, activates these receptors, and affects the downstream PI3K-AKT signaling pathway PMID: 23897819
  5. Myocilin also stimulated osteogenic differentiation of wild-type MSCs, which was associated with activation of the p38, Erk1/2, and JNK MAP kinase signaling pathways PMID: 23629661
  6. We suggest that intracellular myocilin plays a role as a regulator of muscle hypertrophy pathways, acting through the components of dystrophin associated protein complex. PMID: 22371502
  7. Results suggest that expression of mutated myocilins may have a sensitization effect to oxidative stress, which can lead to a severe open-angle glaucoma phenotype in combination with oxidative stress. PMID: 20382707
  8. The TIGR is implicated in resistance to oxidative stress. Despite the presence of a SOD motif, which is necessary for protection in mammalian cells, the protein is not a functional SOD, but might be involved in SOD activity. PMID: 20045724
  9. TIGR is a newly identified component of the CNS glial scar that is likely to contribute to neuronal regenerative failure characteristic of the mammalian CNS. PMID: 12799138
  10. Results do not support a causative role for increased MYOC levels or the MYOC gene in steroid-induced glaucoma. PMID: 15456875
  11. Results show that myocilin and gamma-synuclein interact and as a result, myocilin's properties are changed. PMID: 16392033
  12. These data suggest that production, apparent misfolding, and nonsecretion of mutant MYOC are not, by themselves, sufficient to cause glaucoma in vivo. PMID: 16954374
  13. Tg animals expressed Myoc in tissues of the irido-corneal angle and the sclera. Expression of mutated Myoc induced the accumulation of Myoc in cell cytoplasm and prevented its secretion into the extracellular space. PMID: 17108164
  14. Expression of equivalent levels of mutated human or mouse myocilin in the eyes of transgenic mice produce comparable pathologic changes that are similar to those observed in patients with glaucoma. PMID: 18436825
  15. myocilin induced the formation of stress fibers; myocilin modulates Wnt signaling by interacting with components of signaling pathwaysin the eye PMID: 19188438

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Subcellular Location Secreted, Golgi apparatus, Cytoplasmic vesicle, Secreted, extracellular space, Secreted, extracellular space, extracellular matrix, Secreted, exosome, Mitochondrion, Mitochondrion intermembrane space, Mitochondrion inner membrane, Mitochondrion outer membrane, Rough endoplasmic reticulum, Cell projection, Cell projection, cilium, Note=Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum, It is only imported to mitochondria in the trabecular meshwork, Localizes to the Golgi apparatus in Schlemm's canal endothelial cells, Appears in the extracellular space of trabecular meshwork cells by an unconventional mechanism, likely associated with exosome-like vesicles, Localizes in trabecular meshwork extracellular matrix, SUBCELLULAR LOCATION: Myocilin, C-terminal fragment: Secreted, SUBCELLULAR LOCATION: Myocilin, N-terminal fragment: Endoplasmic reticulum
Tissue Specificity Expressed in ciliary body, iris, retina, trabecular network and sclera but not in lens or cornea. Also expressed strongly in skeletal muscle and weakly in heart, brain, testis, liver, kidney, thyroid and epididymis. No expression detected in embryo. Expre
Database Links

KEGG: mmu:17926

STRING: 10090.ENSMUSP00000028020

UniGene: Mm.10694

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