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Recombinant Escherichia coli Maltose-binding periplasmic protein (malE)

Recombinant Escherichia coli Maltose-binding periplasmic protein (malE)

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Code	CSB-EP360183ENV
Abbreviation	Recombinant E.coli malE protein
MSDS	MSDS Datasheet
Size	US$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360183ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) malE. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360183ENV could indicate that this peptide derived from E.coli-expressed Escherichia coli (strain K12) malE. (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

malE

Uniprot No.

P0AEX9

Research Area

Tags & Cell Markers

Alternative Names

malE; b4034; JW3994; Maltose/maltodextrin-binding periplasmic protein; MMBP; Maltodextrin-binding protein; Maltose-binding protein; MBP

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

27-396aa

Target Protein Sequence

KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

48.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Our Recombinant Escherichia coli malE protein is an exceptional choice for researchers focusing on tags and cell markers. This full-length mature Maltose/maltodextrin-binding periplasmic protein (MMBP; Maltodextrin-binding protein; Maltose-binding protein) is derived from the Escherichia coli (strain K12) species and has an expression region of 27-396aa.

Expressed in E. coli, the malE protein is designed with N-terminal 10xHis and C-terminal Myc tags for seamless detection and purification processes. Boasting a purity greater than 90% as assessed by SDS-PAGE, the Recombinant Escherichia coli malE protein is supplied as a lyophilized powder, ensuring a reliable and high-quality product for your research needs.

Customer Reviews and Q&A

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Target Background

Function

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.

Gene References into Functions

Upon refolding, MBP initially collapses into a dynamic molten globule-like ensemble, then forms an obligatory on-pathway native-like folding intermediate that brings together sequentially remote segments and then folds globally after a long delay. PMID: 29295923
Our current understanding of what makes MBP such an effective solubility enhancer for other proteins and how it facilitates the proper folding of its fusion partners is presented [REVIEW] PMID: 28132493
Maltose Binding Protein is key to the coupling of maltose transport to ATP hydrolysis in vivo, because it facilitates the progression of the MalK dimer from the open to the semi-open conformation, from which it can proceed to hydrolyze ATP PMID: 26268698
activates mouse macrophages and polarizes them into M1 macrophages PMID: 24825603
a salt-bridge motif in the maltose-binding protein has a role in the initial steps of substrate transport PMID: 16143635
Our findings indicate that the solubility-enhancing activity of MBP is mediated by its open conformation and point to a likely role for the ligand-binding cleft in the mechanism of solubility enhancement. PMID: 17964542
These data indicate that unfolding of the mature domain of preMBP is likely not a rate-determining step in translocation when the protein is targeted to the translocase via SecB. PMID: 18241889
tat mutants are able to transport maltose in the absence of malE. PMID: 18385983