The Human Vitamin D-binding protein (DBP) ELISA Kit is used to detect and quantify the concentrations of DBP in serum, plasma, and tissue homogenates. This kit exclusively recognizes human DBP protein. It adopts the Competitive ELISA technique in which HRP-conjugated DBP and DBP in samples or standards compete for binding to the pre-coated DBP. And the chromogenic reaction is triggered after the addition of TMB substrate solution. After adding the stop solution, the color development is immediately terminated and the color turns from blue to yellow. The intensity of the color is negatively relevant to the levels of DBP in the sample. This ELISA kit has been confirmed to have high sensitivity, excellent specificity, premium precision, high recovery, and lot-to-lot consistency. See the product instructions for more details.
DBP, also known as Group-specific Component (GC), is the key transport protein for vitamin D metabolites circulating in the blood. It is primarily produced by hepatic parenchymal cells and secreted into the blood circulation. DBP also transports fatty acids and extracellular actin. Additionally, DBP is also involved in the control of bone development and may participate in modulating immune and inflammatory responses.