Human Vitamin D-binding protein,DBP ELISA Kit

Code CSB-E11859h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human Vitamin D-binding protein (DBP) ELISA Kit is used to detect and quantify the concentrations of DBP in serum, plasma, and tissue homogenates. This kit exclusively recognizes human DBP protein. It adopts the Competitive ELISA technique in which HRP-conjugated DBP and DBP in samples or standards compete for binding to the pre-coated DBP. And the chromogenic reaction is triggered after the addition of TMB substrate solution. After adding the stop solution, the color development is immediately terminated and the color turns from blue to yellow. The intensity of the color is negatively relevant to the levels of DBP in the sample. This ELISA kit has been confirmed to have high sensitivity, excellent specificity, premium precision, high recovery, and lot-to-lot consistency. See the product instructions for more details.

DBP, also known as Group-specific Component (GC), is the key transport protein for vitamin D metabolites circulating in the blood. It is primarily produced by hepatic parenchymal cells and secreted into the blood circulation. DBP also transports fatty acids and extracellular actin. Additionally, DBP is also involved in the control of bone development and may participate in modulating immune and inflammatory responses.

Alternative Names DBP ELISA Kit; DBP/GC ELISA Kit; GC ELISA Kit; Gc globulin ELISA Kit; Gc-globulin ELISA Kit; GRD3 ELISA Kit; Group specific component ELISA Kit; Group specific component vitamin D binding protein ELISA Kit; Group-specific component ELISA Kit; hDBP ELISA Kit; VDB ELISA Kit; VDBG ELISA Kit; VDBP ELISA Kit; Vitamin D binding alpha globulin ELISA Kit; Vitamin D-binding protein ELISA Kit; VTDB_HUMAN ELISA Kit
Abbreviation GC
Uniprot No. P02774
Species Homo sapiens (Human)
Sample Types
Detection Range
Sensitivity
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Materials provided
    • A 96-well Assay plate --The 96-well plate has been pre-coated with human DBP.
    • Standard(Freeze-dried) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-conjugated DBP antibody(100 x concentrate) (1 x 60 μl) --Bind to the DBP, and HRP catalyzes the TMB to elicit a chromogenic reaction.
    • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated DBP antibody solution.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
    • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Gene References into Functions
  1. VDBP in the cervicovaginal fluid (CVF) independently predicts intra-amniotic infection and imminent preterm delivery in women with PTL, whereas in women with preterm premature rupture of membranes, an elevated VDBP level in CVF is not associated with increased risks of these two outcome variables. PMID: 29879190
  2. SNPs of the VDR and GC genes are associated with vitamin D deficiency in postmenopausal Mexican women. PMID: 30150596
  3. GC rs7041 genotype modified the effects of pregnancy on maternal and placental vitamin D metabolism. PMID: 29196501
  4. Urinary VDBP correlated with proteinuria and renal SLE disease activity index, and predicted the development of proteinuria in lupus nephritis. PMID: 29958502
  5. Findings implied that VDBP rs7041-G and rs3733359-T variants may contribute to increased susceptibility to HCV infection in a high-risk Chinese population. PMID: 30218750
  6. Polymorphisms of VDBP rs4588 and rs2282679 may play a potentially important role in epilepsy susceptibility. PMID: 29993274
  7. The study findings suggested a possible clinical application of uVDPB as an early and a good marker for the detection of early renal disease in type 2 diabetes mellitus Saudi patients. PMID: 29850609
  8. GC gene variant has no effect on 25-hydroxyvitamin D levels. PMID: 28892641
  9. genetic association study in population in north India: Data suggest (1) GT allele of VDBP SNP rs7041, (2) VDBP allelic combination (GC1F/1F: T allele rs4588; C allele rs7041), and (3) GA allele of CYP2R1 SNP rs2060793 are associated with vitamin D deficiency in women with PCOS (polycystic ovarian syndrome) in population studied. (VDBP = vitamin D-binding protein; CYP2R1 = cytochrome P450 family 2 subfamily R member 1) PMID: 28008453
  10. The A allele of VDBP gene polymorphism might be a potential risk factor for progression of chronic urticarial. PMID: 29165650
  11. The present study indicates an association between VDR and vitamin D binding protein Single Nucleotide Polymorphisms and Type 1 Diabetes Mellitus among Turkish subjects. PMID: 29506625
  12. Survival analyses showed that the vitamin D binding protein C allele was correlated with poor disease-free survival (DFS). PMID: 29409465
  13. These findings showed that racial/ethnic variations in bioavailable vitamin D do not explain the lack of association between 25-hydroxyvitamin D and multiple sclerosis in blacks and Hispanics; and they further challenge the biological plausibility of vitamin D deficiency as causal for MS. PMID: 29414925
  14. We observed associations between VDR, GC, and CYP27B1 variants and maternal 25-hydroxyvitamin D concentration. Our results provide additional support for a possible role of genetic variation in vitamin D metabolism genes on vitamin D status during pregnancy. PMID: 29175129
  15. Data show that vitamin D-binding protein (DBP) is elevated in the CSF of temporal lobe epilepsy patients. PMID: 19109932
  16. In a Turkish Parkinson disease cohort, rs7041 of GC was associated with the PD risk. The homozygous major allele carriers for rs2282679, rs3755967 and rs2298850 of GC gene in PD patients with slower progression had significantly higher levels of serum 25OHD. This is the first study demonstrating GC gene as a risk factor for PD. PMID: 27282160
  17. Vitamin D levels are associated with severity of liver fibrosis in chronic hepatitis C genotype 1 patients. Although the rs7041 and rs4588 GC polymorphisms are strong predictors of vitamin D levels, they do not play a direct role in liver fibrosis. PMID: 28809744
  18. Vitamin D binding protein polymorphisms were frequently associated to fibrosis grade in chronic hepatitis C suggesting that they could be used as disease evaluation markers to understand the mechanisms underlying the virus-host interaction. PMID: 29465575
  19. Findings indicate that Gc globulin (GC) rs16847024, retinoid X receptor gamma (RXRG) rs17429130 and retinoid X receptor alpha (RXRA) rs4917356 were candidate susceptibility markers for gestational diabetes mellitus (GDM) in Chinese females. PMID: 27636996
  20. Increased circulating levels of vitamin D binding protein in multiple sclerosis patients. PMID: 25590278
  21. Results show that APOE, DBP and AGT identified were associated with survival outcomes in metastatic colorectal cancer patients treated with chemotherapy and bevacizumab PMID: 25428203
  22. results provide a direct evidence of cross-talk among the structural domains of DBP PMID: 18035050
  23. DBP strictly inhibited the production of 1alpha,25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3-induced T cell responses. PMID: 25230725
  24. 25(OH)D2 half-life was shorter than 25(OH)D3 half-life, and half-lives were affected by DBP concentration and genotype PMID: 24885631
  25. The interaction between vitamin D status, as measured by circulating 25(OH)D and DBP rs2282679 genotypes, modified the association between 25(OH)D and BMD and bone markers PMID: 25890042
  26. Data suggest that, while low circulating levels of DBP contribute to low circulating levels of 25-hydroxyvitamin D in patients with PHPT (primary hyperparathyroidism), low DBP alone is not responsible for the hypovitaminosis D observed in these patients; vitamin D metabolism is likely to be generally disturbed in PHPT. [EDITORIAL] PMID: 27858283
  27. the purpose of this investigation was to assess the relative degree of O-linked trisaccharide glycosylation of DBP in breast, colorectal, pancreatic, and prostate cancer patients compared with healthy individuals. PMID: 19642159
  28. Comparison of Two ELISA Methods and Mass Spectrometry for Measurement of Vitamin D-Binding Protein: Implications for the Assessment of Bioavailable Vitamin D Concentrations Across Genotypes. PMID: 27250744
  29. Single-nucleotide polymorphisms in the vitamin D binding protein genewere independently associated with lower 25-hydroxy-vitamin Dand higher 24,25-dihydroxy-vitamin D. PMID: 27313313
  30. genetic association studies in a population in Brazil: Data suggest that SNPs in RXRG (rs2134095) and GC (rs7041) are associated with low-density lipoprotein cholesterol levels and hypercholesterolemia in the population studied; there was no apparent association with an SNP in VDR (rs2228570). (RXRG = retinoid X receptor gamma; GC = vitamin D-binding protein; VDR = vitamin D receptor) PMID: 27721113
  31. The study strongly suggests that there might have an association of vitamin D, and vitamin D-binding protein gene (codon 416 & 420) polymorphisms with the occurrence of type 2 diabetes mellitus PMID: 28888576
  32. Low serum vitamin D-binding protein concentrations are associated with type 1 diabetes. PMID: 27103201
  33. rs7041 polymorphism of Vitamin D Binding Protein does not affect platelet reactivity or the rate of high-residual platelet reactivity among patients receiving dual antiplatelet therapy with clopidogrel or ticagrelor. PMID: 28433569
  34. High VDBG concentration was associated with coronary heart disease events in all racial and ethnic groups. PMID: 28472285
  35. SNPs rs7041 and rs4588 of VDBP are not associated with the levels of 25-hydroxyvitamin D nor with the prevalence and extent of CAD. 25-hydroxyvitamin D levels but not VDBP genetic status independently predicted the occurrence of coronary lesions at angiography. PMID: 28779988
  36. Genetic variant in vitamin D-binding protein is associated with metabolic syndrome. PMID: 28278285
  37. Study suggests higher (versus lower) circulating DBP may be independently associated with a decreased prostate cancer risk in black men independent of 25(OH)D status. PMID: 28369777
  38. The data demonstrate a relationship between the diurnal rhythms of 1,25(OH)2D and DBP, possibly to maintain free 1,25(OH)2D concentrations. PMID: 28732681
  39. the interaction between 25(OH)D status and some maternal GC variants influence the birth weight of infants PMID: 28241988
  40. The results of this study suggest that DBP is not involved in the pathogenesis of MS in Italians. PMID: 28284354
  41. This report summerizes current understanding of vitamin D-binding protein, its genetic determinants, and their effect on calcifediol concentrations. (Review) PMID: 27742848
  42. Allelic variations in CYP2R1 and GC affect vitamin D levels, but variant alleles on VDR and DHCR7 were not correlated with vitamin D deficiency. PMID: 26038244
  43. Patients with primary hyperparathyroidism have lower serum VDBP than controls. PMID: 27682354
  44. VDBP polymorphism is associated with vitamin D deficiency. PMID: 27768857
  45. genome-wide association study in population of children/adolescents in Colorado: Data suggest that VDBP is an autoantigen in development of autoimmune type 1 diabetes (T1D) in the population studied; serum 25-hydroxyvitamin D levels are negatively correlated with VDBP-autoantibody levels in patients in whom T1D developed during the winter. [META-ANALYSIS] PMID: 26983959
  46. VDBP rs222020 C > T polymorphisms may be predisposition factors of adolescent idiopathic scoliosis and the efficacy of brace treatment. PMID: 27856225
  47. findings do not support a role of an independent effect of the investigated vitamin D-related gene variants, VDBP and CYP27B1, in the risk of Multiple Sclerosis PMID: 27904983
  48. The polymorphisms in the VDR and VDBP genes appeared to be responsible for host susceptibility to human tuberculosis in a Taiwanese population. PMID: 26869016
  49. No association was observed between GC or VDR polymorphisms and breast cancer risk. associations between vitamin D-related genetic variants and breast cancer were not observed overall, although the relationships between vitamin D pathway polymorphisms and breast cancer may be modified by menopausal status and breast tumour subtype PMID: 26631034
  50. In a Pakistani population, no statistically significant associations between SNPs in VDR, DBP, and CYP2R1 and tuberculosis was demonstrated. PMID: 27160686

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Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.
Database Links

HGNC: 4187

OMIM: 139200

KEGG: hsa:2638

STRING: 9606.ENSP00000421725

UniGene: Hs.418497

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