Human Vitamin D-binding protein,DBP ELISA Kit

Code CSB-E11859h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P02774
Lead Time 3-5 working days
Abbreviation GC
Protein Biological Process 1 Transport
Target Name group-specific component (vitamin D binding protein)
Alias DBP, DBP/GC, GRD3, VDBG, VDBP, vitamin D-binding alpha-globulin|vitamin D-binding protein
Species Homo sapiens (Human)
Protein Biological Process 3 Transport
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.039 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This protein belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues.
HGNC 7940
RGD 3194
MGI 97368
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human DBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 87  
Range % 82-93  
1:40 Average % 94  
Range % 89-99  
1:80 Average % 92  
Range % 87-96  
1:160 Average % 95  
Range % 90-100  
Recovery
The recovery of human DBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 85-97  
EDTA plasma (n=4) 96 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
10 0.102 0.104 0.103    
5 0.155 0.151 0.153    
2.5 0.282 0.293 0.288    
1.25 0.548 0.541 0.545    
0.625 0.985 0.923 0.954    
0.312 1.328 1.256 1.292    
0.156 1.725 1.598 1.662    
0 2.614 2.444 2.529    
References
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Function Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.
Database Links

HGNC: 4187

OMIM: 139200

KEGG: hsa:2638

STRING: 9606.ENSP00000421725

UniGene: Hs.418497

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