Plant hormone abscisic acid,ABA ELISA Kit

Code CSB-E09159Pl
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation ABA
Protein Biological Process 1 others
Target Name hormone abscisic acid,ABA
Species Plant
Sample Types plant tissues
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.04 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Recovery
The recovery of plant ABA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Xylem saps from plants (n=5) 95 89-98  
Crude extracts (n=5) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
10 0.124 0.126 0.125    
5 0.213 0.228 0.221    
2.5 0.357 0.361 0.359    
1.25 0.498 0.506 0.502    
0.625 0.686 0.701 0.694    
0.312 1.244 1.268 1.256    
0.156 1.510 1.534 1.522    
0 2.241 2.297 2.269    
References
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Q&A and Customer Reviews

Q&A
Q:

When the samples are ground, does it matter how quickly they are processed – I have hundreds of samples and I think I will have to grind at least 96/per kit before processing a set. Is the time taken after grinding before processing further limited, just in case the sample changes its chemical composition?
My samples are wheat leaves grown under drought conditions. Some of them are small collections and I am concerned they may not reach 0.5 g as stated in the protocol. If say the sample is less than 05.g, e.g. 0.4g, does that mean I will need to use 3.6 ml of the extraction buffer, since 0.5 g to 4.5 ml is 1: 9 in ratio terms.

A:
Thanks for your inquiry. There is no strict requirement on the grind speed, but it would be better to grind the sample as faster as you can.
It is recommended to grind the sample well and then process further on testing soon.
As you mentioned that you need to prepare large QTY sample, here advice you to store the first prepared sample at 2-8 ℃。 Pls kindly note this just can be stored for one day.
If you do not make the experiment at the same time, really advice you to prepare the sample partially according to your test request.
You can check your experiment schedule and control the time for this.
In theory, the sample volume can be adapted. Generally it is recommended to take 0.5g to prepare the sample. If it is too less, the sample will not be detected.
And dear, if your sample is really not abundant, here are 2 advice: first is that pls try to collect more sample, second is that change the sample based on the ratio terms 1:9.
Q:

The freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried – please state for how this needs to be. In addition, how is the grinding performed? What tools are used?

A:
Thanks for your inquiry.
Yes, the freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried. You already keep them at -80 degrees Celsius, the samples are through the freeze-dried process indeed.
When do make the test, pls take 0.5g out and use the mortar usually used in lab to grind the freeze-dried samples.
And then check the detailed steps"Crude extracts " according to the manual to arrange the preliminary experiment.
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