Plant hormone abscisic acid,ABA ELISA Kit

Code CSB-E09159Pl
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation ABA
Protein Biological Process 1 others
Target Name hormone abscisic acid,ABA
Species Plant
Sample Types plant tissues
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.04 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<10%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<20%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Recovery
The recovery of plant ABA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Xylem saps from plants (n=5) 95 89-98  
Crude extracts (n=5) 95 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
10 0.124 0.126 0.125    
5 0.213 0.228 0.221    
2.5 0.357 0.361 0.359    
1.25 0.498 0.506 0.502    
0.625 0.686 0.701 0.694    
0.312 1.244 1.268 1.256    
0.156 1.510 1.534 1.522    
0 2.241 2.297 2.269    
References
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