Plant hormone abscisic acid,ABA ELISA Kit

Thanks for your inquiry. There is no strict requirement on the grind speed, but it would be better to grind the sample as faster as you can.
It is recommended to grind the sample well and then process further on testing soon.
As you mentioned that you need to prepare large QTY sample, here advice you to store the first prepared sample at 2-8 ℃。 Pls kindly note this just can be stored for one day.
If you do not make the experiment at the same time, really advice you to prepare the sample partially according to your test request.
You can check your experiment schedule and control the time for this.
In theory, the sample volume can be adapted. Generally it is recommended to take 0.5g to prepare the sample. If it is too less, the sample will not be detected.
And dear, if your sample is really not abundant, here are 2 advice: first is that pls try to collect more sample, second is that change the sample based on the ratio terms 1:9.

Thanks for your inquiry.
Yes, the freshly stored wheat leaf samples at -80 degrees Celsius will need to be freeze-dried. You already keep them at -80 degrees Celsius, the samples are through the freeze-dried process indeed.
When do make the test, pls take 0.5g out and use the mortar usually used in lab to grind the freeze-dried samples.
And then check the detailed steps"Crude extracts " according to the manual to arrange the preliminary experiment.