Code | CSB-RA776663A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The F3 recombinant monoclonal antibody synthesis involves protein technology and DNA recombinant technology. Initially, mice are immunized with a synthesized peptide from human tissue factor. After some time, the spleen of the mice is extracted aseptically and the total RNA of spleen cells is obtained. The cDNA synthesized through RNA reverse transcription is then used as a template to amplify the F3 antibody gene using PCR. The obtained gene is introduced into a vector, which is then transfected into host cells and cultured. The F3 recombinant monoclonal antibody is purified from the supernatant of cell culture using affinity chromatography. It has undergone rigorous verification and is suitable for human and rat F3 protein detection in ELISA, WB, and IHC experiments.
Coagulation factor III (F3), also known as tissue factor (TF), is a transmembrane protein that plays a critical role in the initiation of the coagulation cascade, which is essential for the formation of blood clots to stop bleeding. In response to tissue injury, TF is exposed to the bloodstream and forms a complex with coagulation factor VII, which is activated to factor VIIa by a tissue factor-dependent protease. This TF-FVIIa complex then activates the downstream factors in the coagulation cascade, leading to the generation of thrombin and the formation of a blood clot. TF has been shown to have additional functions, including regulation of angiogenesis, inflammation, and cell survival. TF has also been implicated in various disease states, including thrombotic disorders and cancer.
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