Dog Interleukin 6,IL-6 ELISA Kit

Instructions
Code CSB-E11260c
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 6 (interferon, beta 2)
Alternative Names IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
Abbreviation IL6
Uniprot No. P41323
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 pg/mL-100 pg/mL
Sensitivity 0.39 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 84  
Range % 80-88  
1:2 Average % 95  
Range % 89-105  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 93  
Range % 89-97  
Recovery
The recovery of dog IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-96  
EDTA plasma (n=4) 93 85-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
100 2.954 2.989 2.972 2.855  
50 2.388 2.386 2.387 2.270  
25 1.758 1.796 1.777 1.660  
12.5 1.035 1.106 1.071 0.954  
6.25 0.502 0.513 0.508 0.391  
3.12 0.271 0.268 0.270 0.153  
1.56 0.184 0.196 0.190 0.073  
0 0.112 0.121 0.117    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions
  1. An increase in ventricular dimension is accompanied by increases in AQP4 and IL-6 concentrations in the CSF of dogs with idiopathic communicating hydrocephalus. All were greatly reduced or normalised after shunt treatment. Therefore AQP4 and IL-6 in CSF can be indicators of ventriculomegaly, of cellular damage, and of improvement after the treatment of hydrocephalus. PMID: 27357498
  2. The results of this study suggest that IL-6 is a useful prognostic marker for canine critical care patients with acute internal disease. PMID: 25866911
  3. This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol. PMID: 25312048
  4. Release of interleukin-6 and substance P may initiate proinflammatory effects leading to development of persistent pain in dogs with syringomyelia. PMID: 23659719
  5. The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported. PMID: 22381588
  6. There was no association between IL6 variants and periodontal disease in dogs. PMID: 21708229
  7. These findings suggest that IL-6 secretion during acute urinary tract infection may serve a dual biological role by initiating the inflammatory response while also repairing urothelial defenses. PMID: 21115724
  8. Tumor-infiltrating lymphocytes isolated from regressing canine transmissible venereal tumor secrete high concentrations of IL-6 and antagonize the anti-lymphokine-activated killer activity of tumor cell transforming growth factor-beta 1. PMID: 14734728
  9. Plasma IL-6 concentration is predictive of outcome in canine systemic inflammatory response syndrome and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs. PMID: 17806073
  10. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642

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Subcellular Location Secreted
Protein Families IL-6 superfamily
Database Links

KEGG: cfa:403985

STRING: 9615.ENSCAFP00000004016

UniGene: Cfa.3528

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