The fusion tag N-terminal 10xHis tag and C-terminal Myc tag gene was added to the gene sequence corresponding to the E.coli of the mouse Lta protein to form the recombinant DNA. The recombinant DNA was cloned into the expression vector and then transformed into the E.coli for expression. Following purification, the product is the recombinant mouse Lta protein carrying N-terminal 10xHis tag and C-terminal Myc tag. The SDS-PAGE assessed the purity of this recombinant Lta protein up to 85%. It had an apparent molecular weight of approximately 24 kDa. This recombinant Lta protein may have applications in the research of Lta-associated cancer.
Lta is a gene providing instructions for making a protein named Lymphotoxin-alpha in mice and belongs to tumor necrosis factor family. This protein is a cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. Reported diseases associated with LTA include Psoriatic Arthritis and Leprosy 4. Studies have reported that LT-alpha-deficient mice can generate antigen-specific CD8 T cells in response to infection with influenza A virus. Furthermore, various types of LTA have been reported to stimulate NO production in in vitro cultures of mouse macrophages, rat macrophages, and vascular smooth muscle cells.
||Greater than 85% as determined by SDS-PAGE.
||Lta; Tnfb; Tnfsf1; Lymphotoxin-alpha; LT-alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1
||Mus musculus (Mouse)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
||Full Length of Mature Protein
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.