Recombinant Regulatory protein E2 (E2)

Code CSB-YP355956BKJ
MSDS
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Source Yeast
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Code CSB-EP355956BKJ
MSDS
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Source E.coli
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Code CSB-EP355956BKJ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP355956BKJ
MSDS
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Source Baculovirus
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Code CSB-MP355956BKJ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
E2
Uniprot No.
Alternative Names
E2; Regulatory protein E2
Species
Bovine papillomavirus type 1
Expression Region
1-410
Target Protein Sequence
METACERLHV AQETQMQLIE KSSDKLQDHI LYWTAVRTEN TLLYAARKKG VTVLGHCRVP HSVVCQERAK QAIEMQLSLQ ELSKTEFGDE PWSLLDTSWD RYMSEPKRCF KKGARVVEVE FDGNASNTNW YTVYSNLYMR TEDGWQLAKA GADGTGLYYC TMAGAGRIYY SRFGDEAARF STTGHYSVRD QDRVYAGVSS TSSDFRDRPD GVWVASEGPE GDPAGKEAEP AQPVSSLLGS PACGPIRAGL GWVRDGPRSH PYNFPAGSGG SILRSSSTPV QGTVPVDLAS RQEEEEQSPD STEEEPVTLP RRTTNDGFHL LKAGGSCFAL ISGTANQVKC YRFRVKKNHR HRYENCTTTW FTVADNGAER QGQAQILITF GSPSQRQDFL KHVPLPPGMN ISGFTASLDF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in the initiation of viral DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is removed from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2RE's position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex.
Gene References into Functions
  1. Results for this mutational analysis suggest that the transactivation domain dimerization interface may contribute to the DNA replication activity of bovine papillomavirus type 1 E2 protein PMID: 23490049
  2. Authors propose a model by which bovine papillomavirus E2 transcriptional activity is controlled by bovine p300-mediated acetylation of lysine 111. PMID: 23152516
  3. Thus, the bovine papillomavirus-1 E2-TR modulates viral gene expression in a manner distinct from other E2 proteins. PMID: 22551766
  4. These data provide evidence that the association of bovine papillomavirus E2 with human ChlR1 contributes to a loading mechanism during DNA replication rather than direct tethering during mitotic division. PMID: 21489590
  5. results indicate that precise folding of the E2 transactivation domain is crucial for its interaction with mitotic chromosomes and that this association can be modulated PMID: 15650176
  6. Brd4 has recently been proposed to mediate the association between bovine papilomavirus1 E2 and mitotic chromosomes. PMID: 15795266
  7. E2 proteins that could dimerize were able to bind to mitotic chromosomes much more efficiently than monomeric E2 polypeptides. PMID: 18495759
  8. E2 heterodimers with single activation domain are functional in initiation of papillomavirus replication in vivo. PMID: 19232665
  9. BPV E2 remaines attached to definable segments of the viral genome at specific stages of the cell cycle. PMID: 19716579

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Subcellular Location
Host nucleus. Note=Colocalizes with DDX11 at early stages of mitosis.
Protein Families
Papillomaviridae E2 protein family
Database Links

KEGG: vg:1489020

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