Human Ceruloplasmin,CP/CER ELISA Kit

Instructions
Code CSB-E08383h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name ceruloplasmin (ferroxidase)
Alternative Names CERU_HUMAN ELISA Kit; Ceruloplasmin ELISA Kit; CP 2 ELISA Kit; CP ELISA Kit; CP2 ELISA Kit; Ferroxidase ELISA Kit
Abbreviation CP
Uniprot No. P00450
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, urine, saliva
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:50000Average %93
Range %90-98
1:100000Average %92
Range %87-97
1:200000Average %95
Range %91-103
1:400000Average %94
Range %86-100
Recovery
The recovery of human CP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9689-102
EDTA plasma (n=4)9388-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.750 2.816 2.783 2.625
52.151 2.114 2.133 1.975
2.51.368 1.390 1.379 1.221
1.250.876 0.911 0.894 0.736
0.6250.545 0.568 0.557 0.399
0.3120.382 0.405 0.394 0.236
0.1560.281 0.289 0.285 0.127
00.158 0.157 0.158  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense (By similarity).
Gene References into Functions
  1. ceruloplasmin as a biomarker of chronic kidney disease in urine of sickle cell disease patients PMID: 29127684
  2. Maternal blood ceruloplasmin level was found to be lower in neural tube defect-affected pregnancies as compared to healthy controls. PMID: 28397206
  3. Increased serum transferrin and ceruloplasmin turnover in diet-controlled patients with type 2 diabetes has been reported. PMID: 29079528
  4. Strong correlations between the meconium concentrations of CP, LF and MPO indicate a possible role of these complementary proteins in maintaining homeostasis of the intrauterine environment of the fetus. CP, LF and MPO measured in meconium may serve as biomarkers for assessment of impairment of oxidative balance during intrauterine life with its potential impact on disease development in adulthood. PMID: 27903408
  5. A woman in her 50s who initially presented with movement disorder was diagnosed with aceruloplasminemia, a rare autosomal recessive disorder resulting in accumulation of iron deposits in major organs. Genetic testing confirmed mutations in the ceruloplasmin gene (c.1864 + 1G>C). PMID: 27416276
  6. Higher circulating ceruloplasmin levels were associated with increased atrial fibrillation risk. PMID: 28427851
  7. for ATP7B mutations, the more severe impact on ATP7B protein was, the younger onset age and lower Cp level presented. The feasibility of presymptomatic DNA diagnosis and predicting clinical manifestation or severity of Wilson disease (WD) would be facilitated with identified mutations and genotype-phenotype correlation precisely revealed in the study. PMID: 27982432
  8. Ceruloplasmin is supposed to be related with advanced T stage and perineural invasion, having a possibility as a candidate prognostic marker for bile duct cancer. PMID: 28423673
  9. Mechanistically, ceruloplasmin could specifically interact with the hepatitis B virus middle surface protein. PMID: 28678687
  10. The present study determined that ARE, CLP, CAT, and MPO levels are different between the pediatric patients with sepsis and healthy controls. ARE level can be a potent biomarker for sepsis in critical patients in intensive care units. PMID: 28167245
  11. Ceruloplasmin was independently and negatively associated with liver fibrosis in chronic hepatitis b. PMID: 27920479
  12. Coronary atherosclerosis is distinguished by serum C4 complement up-regulation and ceruloplasmin down-regulation. PMID: 28091899
  13. Data show that the concentration of ceruloplasmin (ferroxidase; Cp) was significantly higher in Low hemoglobin (Hb) compared to High Hb subsample. PMID: 27235174
  14. serum ceruloplasmin level was lower in the primary open-angle glaucoma group in comparison to the group with only cataract. PMID: 27109647
  15. The determination of serum ceruloplasmin in adolescents might be a useful tool to identify patients with the highest risk of future cardiovascular disease. PMID: 27083758
  16. The main result of this study is that ceruloplasmin specific activity is associated with a decreased risk of developing Alzheimer's disease PMID: 26836154
  17. pathological cerebrospinal fluid's environment of Parkinson's disease patients promoted the same modifications in the exogenously added ceruloplasmin PMID: 26537957
  18. Homozygosity for c.1948G>A is described in a Dutch family with aceruloplasminemia. PMID: 25661792
  19. Cp-mediated neuroprotection occurs via the inhibition of seizure-associated oxidative damage. PMID: 25843655
  20. PON-1 and ferroxidase activities in older patients with mild cognitive impairment, late onset Alzheimer's disease or vascular dementia PMID: 25490030
  21. results of this study suggest that common genetic variants of CP are associated with PD and further investigation is needed to explore their functions in Parkinson disease. PMID: 25758665
  22. we have through bioinformatic screening identified ceruloplasmin as a novel adipokine with increased expression in adipose tissue of obese subjects as well as in cells from obesity-associated cancers. PMID: 24676332
  23. sLe(x)/CP ratio tended to be higher for the pancreatic adenocarcinoma PMID: 25595436
  24. brain microvascular endothelial cell -secreted cytokine activity increases the gene expression of neighboring C6 glioma CP, which reciprocally acts on basolateral BMVEC ferroportin to enhance brain iron import PMID: 25311416
  25. High ceruloplasmin levels are associated with preeclampsia. PMID: 25463281
  26. Mercury metallation of the bacterial copper protein azurin is analogous to the one of human cerulopsmin and factor VIII in mercury poisoning. PMID: 25265377
  27. A reduced serum FeOx activity, which can potentially lead to a rise in oxidative stress-induced biomolecular damage, seems to be a shared condition in inflammatory disorders of the central nervous system including MS. PMID: 25398823
  28. This review describes the main role of ceruloplasmin in iron turnover is oxidizing Fe2+ into Fe3+, a process which is essential for iron binding to transferrin (the main iron-transporting protein), as well as to ferritin (the main iron-storage protein) PMID: 24988611
  29. ceruloplasmin and hepcidin differentially regulate iron efflux from brain microvascular endothelial cells PMID: 24533165
  30. The core-fucosylation ratio of ceruloplasmin increases significantly in alcohol-related hepatocellular carcinoma. PMID: 24799124
  31. In CKD patients, increased serum ceruloplasmin, a regulator of nitric oxide activity, is associated with increased risk of long-term adverse cardiovascular events, even after multivariable model adjustment for traditional clinical/biologic risk factors. PMID: 24311705
  32. Ceruloplasmin and gelsolin are closely interacted with the oncogene NF-kappab. PMID: 23925487
  33. Increased urinary excretion of plasma proteins such as IgG, ceruloplasmin and transferrin, with different molecular radii of 55 A or less and different isoelectric points precede development of microalbuminuria in patients with NIDDM and hypertension. PMID: 24256706
  34. The main result of this meta-analysis is that copper not bound to ceruloplasmin appears signi fi cantly increased in subjects with Alzheimer's disease when compared to healthy controls. PMID: 24072069
  35. CP levels correlate negatively and indirectly with inflammation and fibrosis stages in male chronic hepatitis B patients. PMID: 24282481
  36. both Cp NGR sites can deamidate during aging under oxidative conditions, likely as a consequence of oxidative-induced structural changes, thereby promoting a gain of function in integrin binding, signaling, and cell adhesion. PMID: 24366863
  37. Findings indicate a causal role of ceruloplasmin in AF pathophysiology and suggest that ceruloplasmin might be a mediator in a specific inflammatory pathway that causally links inflammatory diseases and incidence of Atrial Fibrillation (AF). PMID: 24118451
  38. A novel homozygous mutation of c.2185 delC in exon 12 was found in ceruloplasmin gene in a patient with aceruloplasminemia. PMID: 23812204
  39. Eosinophil activation occurred in early and late responses after L-ASA NPT in upper airway mucosa of AERD patients, where ApoA1, alpha2M and CP as well as CysLT may be involved in eosinophilic inflammation. PMID: 23786280
  40. The diffusion of an Fe(2+) ion from the inner opening of the human H-chain ferritin to a ferroxidase site located in the interior region of the protein coat is assisted by Thr135, His136 and Tyr137. PMID: 23344859
  41. Ceruloplasmin was significantly elevated in patients with ischemic or nonischemic cardiomyopathy and had linear correlation with C-reactive protein and LVEF PMID: 23781119
  42. Cp was associated with incident heart failure, mortality, and cardiovascular disease in the Atherosclerosis Risk in Communities population. PMID: 23861484
  43. Data suggest that ceruloplasmin (CP) level in serum decreases according to severity of pediatric nonalcoholic fatty liver disease (NAFLD); thus, serum CP may serve as biomarker to discriminate children with severe NAFLD. PMID: 23154483
  44. plasma ceruloplasmin is a potential plasma biomarker of aGvHD, and it also has prognostic value for risk-adapted prophylaxis. PMID: 23505556
  45. Iron efflux from human brain microvasculature endothelial cells ferroportin requires the action of an exocytoplasmic ferroxidase which can be either endogenous hephaestin or extracellular ceruloplasmin. PMID: 23640881
  46. study of effect of occupational exposure to lead among metallurgy workers in Poland on blood levels of transferrin (TRF), ceruloplasmin (CER) and haptoglobin (HPG): Data suggest that blood levels of copper and CER are raised with lead exposure. PMID: 22923205
  47. ceruloplasmin should provide a protective shield against inadvertent oxidant production by myeloperoxidase during inflammation PMID: 23306200
  48. Letter: report mutations in ceruloplasmin promoter in and possible role in iron hemostasis in patients with porphyria cutanea tarda. PMID: 23012398
  49. study to determine if genetic variations identified in CP gene contribute to oesophageal cancer (OC) pathogenesis or susceptibility in Black Xhosa-speaking South African population; statistically significant associations found for 2 of the novel variants with OC in this study and could potentially contribute to disease susceptibility PMID: 21901748
  50. change of the maintenance of three acute phase proteins: ceruloplasmin, alpha1-antitripsin and orosomucoid in an oral fluid and blood plasma at paradontitis and myocardial infarction PMID: 22708402

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Involvement in disease Aceruloplasminemia (ACERULOP)
Subcellular Location Secreted
Protein Families Multicopper oxidase family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

HGNC: 2295

OMIM: 117700

KEGG: hsa:1356

STRING: 9606.ENSP00000264613

UniGene: Hs.558314

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