Cytokines, a class of protein that can regulate cell growth, differentiation and function, play an important role in a variety of physiological and pathological processes, such as immune regulation, inflammatory response, cell proliferation and growth, tissue regeneration and repair. Therefore, cytokines have been widely used in many fields of research.
Cytokines are mainly released by the cells of the immune system to produce an immune response. According to their different functions, they can be divided into different types, mainly including chemokines, interferon, interleukin, tumor necrosis factor and colony-stimulating factor. They can act alone or together or against each other, but ultimately the role of cytokines is to help regulate the immune response. Therefore, the role of cytokines in immunological research is multifaceted. Mainly including the following points:
(1) Immune regulation: cytokines are involved in the physiological processes of immune cells, such as differentiation, proliferation, migration, adhesion, and cell death, thereby regulating the process and amplitude of the immune response.
(2) Immunotherapy: as an immunomodulator, cytokines can be used to activate immunotherapy, inhibit immunotherapy, etc., including various recombinant, synthetic and natural preparations, etc. For example, recombinant interleukin-2 (IL-2) is used for treating kidney cancer, melanoma and malignant lymphoma, and recombinant interleukin-4 (IL-4) is used for treating allergic diseases.
(3) Immunodiagnosis: the occurrence and severity of some immune diseases can be judged by measuring the level of cytokines, for example, measuring tumor necrosis factor α (TNF-α) can help to diagnose rheumatoid arthritis.
(4) Vaccine development: the study on the role of cytokines in the immune process can provide a theoretical basis for vaccine development, for example, the use of recombinant interleukin-12 (IL-12) as a vaccine adjuvant can enhance the effect of the immune response.
Inflammation is a normal and important immune defense mechanism of the body. However, if it is not effectively controlled, the immune system will attack healthy tissues, causing cytokine storm and other body dysfunction problems. Cytokines can stimulate the differentiation and function of inflammatory cells, playing an important role in the diagnosis, evaluation, treatment of inflammatory diseases and the development of related drugs. For example, anti-tumor necrosis factor alpha (TNF-α) antibody are used to treat rheumatoid arthritis and inflammatory bowel disease. In addition, there are many other cytokines such as IL-1, IL-6, IL-8, and IFN-γ are also widely used in inflammation research.
Tumor cells can evade immune surveillance by reducing the function of immune cells and regulating immune response, while cytokines can restore immune surveillance of tumor cells and promote apoptosis of tumor cells, thereby inhibiting cancer development and progression. A large number of clinical studies have found that the cytokines of cancer patients include IL-2, IL-6, IL-8, IL-10, IL-12, IL-18, TNF-α, TGF-β, IFN-γ, macrophage migration inhibitory factor (MIF) and CXCR4, and the research on the signaling pathways and reaction mechanisms mediated by these cytokines is of great significance for the prevention, diagnosis and treatment of tumors.
High Sensitivity & Specificity
The sensitivity is typically at the pg/mL level, suitable for detecting proteins at very low concentrations; characterized by high specificity and excellent repeatability and accuracy, capable of avoiding cross-reactivity with other proteins, ensuring reliable detection results.
High Stability
The components of the cytokine ELISA kits have been optimized through multiple steps, and the stability of each component has been tested against high-temperature denaturation, minimizing the inactivation of antigens and antibodies during long-distance transportation and storage.
Strict Quality Control
Utilizing high-quality raw materials and rigorous quality control processes, each product undergoes three rounds of quality checks, ensuring stable performance and reliable quality, which maximizes the consistency within batches and between batches of the kits.
Extensive Citations
CUSABIO's products and services have been cited in over 23,000 publications, including high-impact journals such as Nature, Cell, Immunity, Nature Medicine, and Cell Metabolism, widely recognized by customers globally.
Sample type: Serum
Sample species: Mouse
Sample dilution: 1: 5
Review: I used CSB-E04741m to detect spontaneous T2DM mice db/db mice. The dilution ratio was 1: 5, the dilution ratio was appropriate and the concentration was moderate. The measured results were accurate and cost-effective!
By Anonymous
Sample type: Tissue homogenate
Sample species: Mouse
Sample dilution: No dilution
Review: I have tested the CSB-E04741m for PR8 virus-infected mouse lung tissue homogenates, the operation is simple and easy to use, the sensitivity is very high, and the test results are very accurate.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Mouse
Sample dilution: No dilution
Review: I used CSB-E04741m to detect TNFa levels in cell supernatant exosomes and mouse supernatants, respectively. The results of the standard curve were good.
By Anonymous
In response to the special needs of industrial clients such as pharmaceutical companies, CUSABIO has launched the IS (Industry Standard) cytokine detection kits. With rich research and development experience and independent research and development platforms, high-performance raw and auxiliary materials are screened, quality is strictly controlled, and multiple quality controls are implemented. The products can be used for CRS detection, residue detection in semi-finished drug processes, and cell function evaluation in pharmacokinetic experiments, development quality control and pharmacodynamic testing of cell therapy drugs.
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