Alternative Names
aur; Zinc metalloproteinase aureolysin; EC 3.4.24.29; Staphylococcus aureus neutral proteinase
Species
Staphylococcus aureus
Expression Region
210-509
Target Protein Sequence
A ATGTGKGVLG DTKDININSI DGGFSLEDLT HQGKLSAYNF NDQTGQATLI TNEDENFVKD DQRAGVDANY YAKQTYDYYK NTFGRESYDN HGSPIVSLTH VNHYGGQDNR NNAAWIGDKM IYGDGDGRTF TNLSGANDVV AHELTHGVTQ ETANLEYKDQ SGALNESFSD VFGYFVDDED FLMGEDVYTP GKEGDALRSM SNPEQFGQPS HMKDYVYTEK DNGGVHTNSG IPNKAAYNVI QAIGKSKSEQ IYYRALTEYL TSNSNFKDCK DALYQAAKDL YDEQTAEQVY EAWNEVGVE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If
you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6%
Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We
recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at
-20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Delivery time
may differ from different purchasing way or location, please kindly consult your local distributors
for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.
Description
Recombinant Staphylococcus aureus Zinc metalloproteinase aureolysin(aur) is a partial length protein expressed with an N-terminal His-tagged or Tag-Free. Its expression region corresponds to 210-509aa of mouse aur protein. Its purity was determined by SDS-PAGE and reached up to 85%. This recombinant aur protein may be used to synthesize antibodies against aur or on the studies of aur-associated signal transduction.
Aureolysin is an extracellular zinc-dependent metalloproteinase expressed by the pathogenic bacterium Staphylococcus aureus. This enzyme can cleave host factors of the innate immune system anf regulae S. aureus secreted toxins and cell wall proteins, resulting in the bacterium's virulence or disease. To catalyze its enzymatic activities, aureolysin requires zinc and calcium which it obtains from the extracellular environment within the host.
