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Recombinant Staphylococcus aureus Zinc metalloproteinase aureolysin (aur)

Recombinant Staphylococcus aureus Zinc metalloproteinase aureolysin (aur)

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Code	CSB-EP305800FKZ
MSDS	MSDS
Size	US$388
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Image	Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP305800FKZ could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus aur. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP305800FKZ could indicate that this peptide derived from E.coli-expressed Staphylococcus aureus aur. (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

aur

Uniprot No.

P81177

Research Area

Cell Biology

Alternative Names

aur; Zinc metalloproteinase aureolysin; EC 3.4.24.29; Staphylococcus aureus neutral proteinase

Species

Staphylococcus aureus

Source

E.coli

Expression Region

210-509aa

Target Protein Sequence

AATGTGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAYNFNDQTGQATLITNEDENFVKDDQRAGVDANYYAKQTYDYYKNTFGRESYDNHGSPIVSLTHVNHYGGQDNRNNAAWIGDKMIYGDGDGRTFTNLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPSHMKDYVYTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGVE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

40.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer，50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Constructing a plasmid that codes for the Staphylococcus aureus aur protein (210-509aa) initiates the generation of the recombinant Staphylococcus aureus aur protein. The constructed plasmid is transformed into 210-509aa cells. Positive e.coli cells are selected and then cultured under conditions that encourage the expression of the gene of interest. The protein is equipped with a N-terminal 10xHis tag and C-terminal Myc tag. After that, affinity purification is employed to isolate and purify the recombinant aur protein from the cell lysate. The purity of the resulting recombinant aur protein is over 85%, determined by SDS-PAGE.

Customer Reviews and Q&A

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■ Q&A

I am looking for this enzyme: metalloproteinase aureolysin (aur) Recombinant Protein
If available, I would like to know the following details:
- Which tag has the protein?
- Is the enzyme active?

Thanks for your inquiry. Recombinant Staphylococcus aureus Zinc metalloproteinase aureolysin(aur)
CSB-YP305800FKZ Yeast; Tag information: N-terminal 6xHis-tagged
CSB-EP305800FKZ E.coli; Tag information: N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP305800FKZ Baculovirus; Tag information: N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP305800FKZ Mammalian cell; Tag information: The tag will be determined in the production process.
Expression region: 210-509aa, full length of mature protein;
Target protein sequence:

We didn't test the activity of this protein. As we provide the full length of mature protein, it is active in theory. But can not 100% guaranteed.

Target Background

Function

Plays an essential role in immune evasion by helping bacteria to resist complement-mediated killing by neutrophils. Inhibits the deposition of host C3b on bacterial surfaces and the release of the chemoattractant C5a by cleaving the central complement protein C3. The cleavage site renders the C3b molecule vulnerable to proteolytic degradation by host regulators. Cleaves and inactivates host SERPINA1, which is an endogenous protease inhibitor essential for controlling neutrophil serine protease elastase. Plays also an essential role in the cleavage and subsequent activation of the serine protease SspA which is involved in colonization and infection of human tissues.

Protein Families

Peptidase M4 family

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