Recombinant Shigella dysenteriae serotype 1 Cysteine protease-like virA (virA)

Code CSB-YP654056SGF
MSDS
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Source Yeast
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Code CSB-EP654056SGF
MSDS
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Source E.coli
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Code CSB-EP654056SGF-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP654056SGF
MSDS
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Source Baculovirus
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Code CSB-MP654056SGF
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
virA
Uniprot No.
Alternative Names
virA; SDY_P211; Cysteine protease-like VirA; EC 3.4.22.-; Effector protein VirA
Species
Shigella dysenteriae serotype 1 (strain Sd197)
Expression Region
1-400
Target Protein Sequence
MQTSNITNYE RNDSSWMSTV KSTTEVSWNK LSFCDVLLKI ITFGIYSPHE TLAEKYSEKK LMDSFSPSLS QDKMDGEFAH ANIDGISIRL CLNKGICSVF YLDGDKIQST QLSSKEYNNL LSSLPPKQFN LGKVHTITAP VSGNFKTHKP APEVIETAIN CCTSIIPNDD YFSVKDTDFN SVWHDIYRDI RASDSNSTKI YFNNIEIPLK LIADLINELG INEFIDSKKE LQMLSYNQVN KIINSNFPQQ DLCFQTEKLL FTSLFQDPAF ISALTSAFWQ SLHITSSSVE HIYAQIMSEN IENRLNFMPE QRVINNCGHI IKINAVVPKN DTAISASGGR AYEVSSSILP SHITCNGVGI NKIETSYLVH AGTLPSSEGL RNAIPPESRQ VSFAIISPDV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Alpha-tubulin-specific protease that is required for entry into epithelial cells and for subsequent intra- and intercellular spreading. Contributes to bacterial entry into epithelial cells by inducing microtubule (MT) destabilization and the formation of membrane ruffles. The membrane ruffling evoked by VirA results from the activation of host rac1, which is associated with the destruction of MT networks. Creates a tunnel inside the host cell cytoplasm by breaking down the microtubule infrastructure. This facilitates the bacterium's movement through the cytoplasm and also helps other bacteria move faster during the invasion of the eukaryotic cell. Is absolutely required for virulence.
Subcellular Location
Secreted.
Protein Families
Protease EspG/VirA family
Database Links

KEGG: sdy:SDY_P211

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