Recombinant Influenza B virus Nucleoprotein(NP)

Code CSB-EP361330IJK
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names NP
Uniprot No. P04665
Research Area Others
Alternative Names NP; Nucleoprotein; Nucleocapsid protein; Protein N
Species Influenza B virus (strain B/Lee/1940)
Source E.coli
Expression Region 1-560aa
Target Protein Sequence MSNMDIDSINTGTIDKTPEELTPGTSGATRPIIKPATLAPPSNKRTRNPSPERTTTSSETDIGRKIQKKQTPTEIKKSVYKMVVKLGEFYNQMMVKAGLNDDMERNLIQNAQAVERILLAATDDKKTEYQKKRNARDVKEGKEEIDHNKTGGTFYKMVRDDKTIYFSPIKITFLKEEVKTMYKTTMGSDGFSGLNHIMIGHSQMNDVCFQRSKGLKRVGLDPSLISTFAGSTLPRRSGTTGVAIKGGGTLVDEAIRFIGRAMADRGLLRDIKAKTAYEKILLNLKNKCSAPQQKALVDQVIGSRNPGIADIEDLTLLARSMVVVRPSVASKVVLPISIYAKIPQLGFNTEEYSMVGYEAMALYNMATPVSILRMGDDAKDKSQLFFMSCFGAAYEDLRVLSALTGTEFKPRSALKCKGFHVPAKEQVEGMGAALMSIKLQFWAPMTRSGGNEVSGEGGSGQISCSPVFAVERPIALSKQAVRRMLSMNVEGRDADVKGNLLKMMNDSMAKKTSGNAFIGKKMFQISDKNKVNPIEIPIKQTIPNFFFGRDTAEDYDDLDY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 77.8kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Q&A and Customer Reviews

 Q&A
Q:

Could you please tell me if there is a His+SUMO tag on this product CSB-EP361330IJK?

A:
Very nice to receive your inquiry. CSB-EP361330IJK is tagged with N-terminal 6xHis-SUMO-tag. The details is as below.
Code: CSB-EP361330IJK
Name: Recombinant Influenza B virus Nucleoprotein(NP)
Expression Region: 1-560aa, full length
Tag Info: N-terminal 6xHis-SUMO-tag
Target Protein Sequence:

MSNMDIDSINTGTIDKTPEELTPGTSGATRPIIKPATLAPPSNKRTRNPSPERTTTSSETDIGRKIQKKQTPTEIKKSVYKMVVKLGEFYNQMMVKAGLNDDMERNLIQNAQAVERILLAATDDKKTEYQKKRNARDVKEGKEEIDHNKTGGTFYKMVRDDKTIYFSPIKITFLKEEVKTMYKTTMGSDGFSGLNHIMIGHSQMNDVCFQRSKGLKRVGLDPSLISTFAGSTLPRRSGTTGVAIKGGGTLVDEAIRFIGRAMADRGLLRDIKAKTAYEKILLNLKNKCSAPQQKALVDQVIGSRNPGIADIEDLTLLARSMVVVRPSVASKVVLPISIYAKIPQLGFNTEEYSMVGYEAMALYNMATPVSILRMGDDAKDKSQLFFMSCFGAAYEDLRVLSALTGTEFKPRSALKCKGFHVPAKEQVEGMGAALMSIKLQFWAPMTRSGGNEVSGEGGSGQISCSPVFAVERPIALSKQAVRRMLSMNVEGRDADVKGNLLKMMNDSMAKKTSGNAFIGKKMFQISDKNKVNPIEIPIKQTIPNFFFGRDTAEDYDDLDY


Q:

I purchased item CSB-EP361330IJK lot 03765 and ask the following questions:
1)What is the exact buffer, including pH of each product?
2)What is the sequence of each product including the tags?
3)How was the concentration determined? What is the concentration of each?
4)What is the purity of each item?

A:
Thank you for your feedback. Except for tag sequence, all other information (Storage Buffer, Concentration, Purity are listed in our Datasheet&COA.
CSB-EP361330IJK lot 03765
1) Storage Buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, 50% glycerol
2) N-terminal 6xHis-SUMO-tag Sequence: (MW: 16 kDa):

MAHHHHHHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGGSEFRT


Target Protein Sequence (MW: 61.8 kDa):
This sequence has been presented on this page.
3) Concentration: 0.4 mg/ml, by the Bradford Method.
4) Purity: 90%, by SDS-PAGE quantitative densitometry by Coomassie Blue Staining.
Q:

I have additional questions about the data that you sent. Would you please help with the questions below?
1). The storage buffer for CSB-EP325878IMI (Flu A) 10% correct? It is not 50%?
2). For Flu A antigen, the buffer contains 0.5M NaCl, while the buffer for Flu B antigen has no salt.

A:
Very nice to receive your inquiry. 1.

MAHHHHHHMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGGSEFRT


The predicted MW of this sequence indeed is 12.96KD. We forgot to explain to the customer and directly provided 16 kDa MW in the last email.
Actually, the MW of SUMO-tag will be about 4kDa larger. Theoretically, because the isoelectric point of the SUMO-tag is very low, the acid protein will generally run relatively larger.
Combined with our actual situation (statistics of not less than a thousand data), the SUMO-tag is indeed about 4 kDa larger, so please don't worry.
There are also many articles reporting that the SUMO-tag shows relative larger size on gel.
2. About the glycerol content in storage buffer: When proteins shipped, we generally add a final concentration of 50% of glycerol by default. Glycerol has a protective effect on the protein.
First, it can increase the stability of the protein (reducing the polarity of the buffer and preventing protein hydrophobic aggregation).
(This is based on the principle of similar dissolution: polar molecules are easily soluble in polar solvents, non-polar molecules are easily soluble in non-polar solvents,
and polar and hydrophilic (water is a polar molecule) correspond, hydrophobic and non-polar Polarity corresponds to a positive correlation.
Second, the freezing point of the system can be reduced, and the protein can be stored at a low temperature. Especially when the protein is freeze-thawed repeatedly,
protein damage (aggregation or inactivation) can be avoided as much as possible.
As for the final concentration of glycerol, in principle, the final concentration of 5% -50% is OK, may involve the preservation of time.
The preservation time of high glycerol content is relatively longer, but for each delivery, this can adjusted according to the customer's request, there is no problem with glycerol plus or minus.
CSB-EP361330IJK is a in-stock protein, and glycerol content is fixed 50%, already added.
3. Different protein/even if the same protein, the stability in different buffers is not the same, and we will choose the optimal buffer, so the buffers of different proteins are not the same.

Target Data

Function Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
Gene References into Functions
  1. Results show that the N terminus is involved in the formation of both higher homo-oligomers of BNP and BNP-RNA complex. PMID: 26368391
Subcellular Location Virion, Host nucleus
Protein Families Influenza viruses nucleoprotein family
Database Links

KEGG: vg:26824002

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