nef Antibody

1. The authors find that both simian immunodeficiency virus and HIV-1 Nef proteins are present in affinity-purified extracellular vesicles derived from cultured cells, as well as in extracellular vesicles from simian immunodeficiency virus-infected macaques. PMID: 29437924
2. Nef regulates viral pathogenesis through interaction with POTEE and mTORC2 activation in macrophages. PMID: 30391463
3. Together these findings suggest a possible mechanism of host invasion by HIV-1 through the CAWLEAQ motif of Nef-mediated regulation of ENO1 and identify a potential therapeutic target for HIV-1 entry at mucosal barriers. PMID: 29404888
4. results demonstrate that HIV-1 Nef's inferior ability to downregulate MHC-B compared to that of MHC-A is conserved across primate lentiviruses and suggest that this property influences antiviral cellular immune responses. PMID: 29046444
5. These results provide direct evidence that Nef promotes cholesterol deposition in the liver aorta. PMID: 27649790
6. HIV-1 Nef protein physically interacts with host proteins that regulate protein trafficking [review]. PMID: 27161574
7. these data suggested that HIV-1 Nef can be a formidable contributor to neurotoxicity along with other factors, which leads to HAND in HIV-1-infected AIDS patients. PMID: 28079886
8. Data suggest that restriction of HIV-1 particle infectivity by host-cell SERINC5 (which is antagonized by viral Nef) does not depend on alterations in lipid composition and organization of HIV-1 particles and suggest that channeling serine into lipid biosynthesis may not be a cardinal cellular function of SERINC5. (SERINC5 = serine incorporator 5; Nef = nef gene product, HIV1gp9) PMID: 28659343
9. These results suggest the involvement of HIV-1 nef and human NKG2D-NKG2D ligand interactions in the enhanced susceptibility of HIV-1-infected cells from HIV elite controllers to antibody-dependent cellular cytotoxicity responses. PMID: 28615199
10. Depletion of the gamma2 or mu1A (AP1M1) subunits of AP-1, but not of gamma1 (AP1G1), precludes Nef-mediated lysosomal degradation of CD4. PMID: 27909244
11. AP-2alpha depletion enhanced apoptosis, demonstrating that disrupting the HIV-1 Nef:AP-2alpha interaction limits Nef-mediated apoptosis. PMID: 28577469
12. findings provide a mechanistic explanation for previous observations that dimerization-defective Nef mutants fail to down-regulate CD4 and validate the Nef dimerization interface as a target site for antiretroviral drug development PMID: 28031466
13. Inhibit interaction between HIV-1 Nef and Calnexin to reverse HIV-induced lipid accumulation and prevent atherosclerosis. PMID: 27470515
14. results suggest that the antagonism of HIV-1 Nef to SERINC5 restriction of virion infectivity is mediated by a dual mechanism that is related to CD4 downregulation PMID: 27681140
15. In light of existing report suggesting critical role of Nef-GCC185 interaction reveals valuable mechanistic insights affecting specific protein transport pathway in docking of late endosome derived Rab9 bearing transport vesicle at TGN elucidating role of Nef during viral pathogenesis. PMID: 27105913
16. The authors found that Akt interacts with HIV-1 Nef protein. In primary T cells treated with exogenous Nef or acutely infected with Nef-expressing HIV-1 in vitro, Akt became phosphorylated on serine(473) and threonine(308). PMID: 27076174
17. Nef exosomes isolated from the plasma of individuals with HIV-associated dementia (HAD) can induce amyloid beta (1-42) secretion in cultured neurons. PMID: 26407718
18. The results show that natural polymorphisms within HIV-1 Nef modulate its interaction with natural polymorphisms in the HLA cytoplasmic tails, thereby affecting the efficiency of HLA downregulation and consequent recognition by HIV-specific T cells. PMID: 26787826
19. HRB knock-down affected CD4 downregulation induced by Nef but not by HIV-1 Vpu. PMID: 26701340
20. The Nef-GPG motif is required for optimal infectivity of those viruses produced in T-cells. PMID: 26700863
21. show that HIV-1 promotes tunneling nanotube formation per se via its protein Nef PMID: 26773158
22. Nef exploits PAK2 in a stepwise mechanism in which its kinase activity cooperates with an adaptor function for the exocyst complex to inhibit host cell actin dynamics. PMID: 26350970
23. Differential signaling mechanism operates in HIV-1 Nef-mediated production of IL-6 and IL-8 in human astrocytes. PMID: 26075907
24. HIV-1 Nef down-regulated CD1a lipid antigen presentation in dendritic cells, and involved both Hck and PAK2. PMID: 26584215
25. TLR2 has a role in the synergistic effect of M. tuberculosis and HIV-1, and their antigens Rv3416 and Nef, respectively, in inhibiting apoptosis of macrophages PMID: 26132135
26. exosomes are unlikely involved in intercellular Nef transfer. PMID: 25919665
27. SERINC3 and SERINC5 together restricted HIV-1 replication, and this restriction was evaded by Nef PMID: 26416733
28. identification of the host transmembrane protein SERINC5, and to a lesser extent SERINC3, as a potent inhibitor of HIV-1 particle infectivity that is counteracted by Nef PMID: 26416734
29. this study reports the cryo-electron microscopy structures of the Nef- and Arf1-bound AP-1 trimer in the active and inactive states. PMID: 26494761
30. Nef expression was necessary for both systemic T cell activation and substantial CD4(+) T cell loss from blood and tissues. PMID: 26169178
31. AP-2 is the most important player for Nef-mediated CD4 downmodulation PMID: 26423947
32. These results lead to a model for the docking of the full AP-2 tetramer to membranes as bound to Nef, such that the cytosolic tail of CD4 is situated to interact with its binding site on Nef. PMID: 24473078
33. our study identifies selectively expressed mRNAs in Nef-expressing U937 cells and their exosomes and supports a new mode on intercellular regulation by the HIV-1 Nef protein. PMID: 25961023
34. unifying picture of Nef functions based on old and new experimental results evidencing that Nef also influence the cytokine network[review] PMID: 25529283
35. Overall study determines modulation of cellular protein expression in T cells by HIV-1 Nef PMID: 25874870
36. Results suggest that Nef protein expressed by transfected astrocytes implanted in rat hippocampus activates the immune system leading to neuronal damage and spatial and recognition memory deficits PMID: 22926191
37. HIV-1 Nef, a key factor for viral pathogenesis, downregulates functionally important molecules from the surface of infected cells, including the viral entry receptor CD4 and coreceptors CCR5 and CXCR4. PMID: 26178998
38. Ile-20 within HIV-1 subtype B Nef is associated with poorer disease outcome. PMID: 26060058
39. Altogether, the findings suggest that the mechanisms of infectivity enhancement by Nef are different between HIV-1 and SIVmac239. PMID: 25519983
40. Nef domains involved in CD4 downregulation were necessary for activation of plasmacytoid dendritic Cell. PMID: 25972534
41. These results support enhanced Nef-mediated HLA class II immune evasion activities in acute/early compared to chronic infection, highlighting the potential importance of these functions following HIV-1 transmission. PMID: 25998395
42. Together, these results suggested that HIV-1 preferential infection of CD4(+)CTLA-4(+) T cells in vivo was followed by Nef-mediated concomitant downregulation of both CD4 and CTLA-4 upon transition to productive infection. PMID: 25626682
43. Taken together, these data indicate that Nef is a critical viral protein for incorporating nascent proviral DNA into host chromosomes in resting peripheral mononuclear leukocytes and that this occurs through interaction with INI1. PMID: 25559666
44. Authors identified a cis element in the 5'UTR of Nef mRNA essential for efficient Nef translation, which was named the Nef-translation essential region (NER). PMID: 24981044
45. Nef-induced apoptosis is mediated through reactive oxygen species-dependent mechanisms. PMID: 24608713
46. Nef markedly activated TAK1 in M-CSF-derived M2-MPhi but not in GM-CSF-derived M1-MPhi. PMID: 24874739
47. Interaction with the Src homology (SH3-SH2) region of the Hck structures the HIV-1 Nef dimer for kinase activation and effector recruitment. PMID: 25122770
48. HIV-1 Nef impairs key functional activities in human macrophages through CD36 downregulation. PMID: 24705461
49. HIV-1 Nef interacts with Alix in late endosomes, and this is required for efficient lysosomal targeting of CD4. PMID: 25118280
50. These results demonstrate that early Nef clones from HIV controllers displayed lower HLA class I and CD4 downregulation activity, as well as a reduced ability to enhance virion infectivity. PMID: 24965469

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KEGG: vg:156110