Mouse Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit

Code CSB-E07918m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name plasminogen activator, tissue
Alternative Names Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit
Abbreviation PLAT
Uniprot No. P11214
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 96
Range % 93-99
1:2 Average % 104
Range % 100-108
1:4 Average % 86
Range % 83-90
1:8 Average % 104
Range % 100-108
Recovery
The recovery of mouse t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 85-93
EDTA plasma (n=4) 102 98-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
8000 2.076 2.000 2.038 1.865
4000 1.453 1.501 1.477 1.304
2000 0.947 0.938 0.943 0.770
1000 0.564 0.559 0.562 0.389
500 0.348 0.351 0.350 0.177
250 0.241 0.246 0.244 0.071
125 0.205 0.200 0.203 0.030
0 0.172 0.174 0.173  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
Gene References into Functions
  1. Sata suggest that tissue-type plasminogen activator (tPA) regulates nocturnal wheel-running activity, and that tPA differentially affects suprachiasmatic nucleus-driven nocturnal activity rhythms and activity driven by fasting or temporal food restriction. PMID: 29370799
  2. Our findings suggest that isoflurane pretreatment could attenuate tPA-exaggerated brain ischemic injury, by reducing tPA-induced LRP/NF-kappaB/Cox-2 in endothelial cells, endothelial MMP-2 and MMP-9 activation, and subsequent pro-apoptotic molecule in neurons after oxygen/glucose deprivation and reperfusion. PMID: 28539818
  3. Tissue plasminogen activator was found to be restricted to subsets of excitatory pyramidal glutamatergic neurons. PMID: 26377106
  4. analysis of mutations in genes co-segregating with the targeted Plat allele, including several potential null mutations PMID: 27079292
  5. By mediating the tPA response in macrophages, the NMDA-R provides a pathway by which the fibrinolysis system may regulate innate immunity. PMID: 28684538
  6. Altogether, this preclinical study demonstrates that the tPA present in the blood stream is a key player of the formation of intracranial aneurysms. PMID: 28754830
  7. A model of sustained hyperfibrinolytic state can be obtained in mice by hydrodynamic transfection of a plasmid encoding for tissue-type plasminogen activator (tPA). PMID: 27531677
  8. While the absence of t-PA confers a more deleterious form of Experimental Autoimmune Encephalomyelitis, neuronal over-expression of t-PA does not overtly protect against this condition with regards to symptom onset or severity of Experimental Autoimmune Encephalomyelitis. PMID: 27427941
  9. mesenchymal stromal cells promote axonal outgrowth via neuronal tPA and synergistically with astrocytic tPA PMID: 27959956
  10. administration of ADAMTS13 5 minutes after occlusion dose-dependently dissolved these t-PA-resistant thrombi resulting in fast restoration of MCA patency and consequently reduced cerebral infarct sizes PMID: 26929275
  11. strategies contributing to promote either endogenous production of tPA or its associated EGFR-linked signaling pathway may have beneficial effects following brain injuries such as stroke. PMID: 26068590
  12. Plasminogen activation contributes to wound repair and suggest that adenosine A2A receptor activation promotes wound closure by a mechanism that depends on tPA. PMID: 25991438
  13. Myeloid-derived Tissue-type plasminogen activator (tPA) promotes macrophage migration through a novel signaling cascade involving FAK, Rac-1, and NF-kappaB. PMID: 25131752
  14. the protective effect of neuroserpin maybe independent from its canonical interaction with a tissue-type plasminogen activator PMID: 26176694
  15. Erythropoietin protects mouse renal tubular basement membrane by promoting bone marrow cells to generate and secrete miR-144, which, in turn, inhibits activation of the tPA/MMP9-mediated proteolytic network. PMID: 26469975
  16. tPA activity in V1 can be unmasked following 4 d of monocular deprivation when mice older than 2 months are raised in standard cages by the genetic removal of Lynx1, a negative regulator of adult plasticity. PMID: 26377459
  17. Results demonstrate that synaptic potentiation results in tissue plasminogen activator-dependent reelin processing and suggest that extracellular proteolysis of reelin may regulate reelin signaling in the adult brain PMID: 24892761
  18. data implicate MCP-1 as the key molecule governing tPA-induced fluid accumulation. The role of MCP-1 in the development of other exudative effusions warrants examination. PMID: 25474480
  19. Data indicated that suggest that tPA modulates emotion in a social context through the function of the prefrontal cortex. PMID: 25499620
  20. in vivo and in vitro evidence showing that this aggravation is correlated with a decrease in lipoprotein receptor-related protein 1-mediated hepatic clearance of tPA in alcohol-exposed mice. PMID: 25922513
  21. Data indicate that closed head trauma sequentially releases tissue-type plasminogen activator (tPA) followed by delayed synthesis and release of urokinase plasminogen activator (uPA) from injured brain. PMID: 25673638
  22. tPA promoted M1 macrophage survival through its receptor LRP-1-mediated novel signaling cascade involving Erk1/2, p90RSK, and p38, which leads to the accumulation of these cells in the injured kidneys PMID: 25670857
  23. gene deficiency results in aggravation of experimental autoimmune myasthenia gravis PMID: 24423642
  24. plg-/- and tPA-/- mice exhibit brain parenchymal fibrin deposits that appear to result from reduced neurovascular integrity PMID: 24612416
  25. Neural progenitor cells from tPA knock-out mice show impaired neurite outgrowth compared to wild-type. PMID: 23925701
  26. Data suggest that tPA is required for bone repair and osteoblast proliferation; bone repair of the femur is delayed in tPA knockout mice, unlike that in wild-type mice (or uPA [urokinase-type plasminogen activator] knockout/wild-type mice). PMID: 24918201
  27. Endogenously released tPA promotes neutrophil transmigration to reperfused tissue via proteolytic activation of plasmin and gelatinases. PMID: 24764453
  28. TPA played an essential role in augmenting circulating endothelial progenitor cells, angiogenesis, and blood flow in the ischemic limb in a murine model. PMID: 24290069
  29. tPA is sufficient to induce interleukin-1beta and phosphorylated extracellular signal-regulated kinase (ERK) expression in astrocyte cultures. PMID: 23707980
  30. excess tPA/plasmin, through separate downstream molecular mechanisms, regulates postnatal Purkinje neuron (PN) dendritogenesis, synaptogenesis, mitochondrial structure and function, and selective PN viability PMID: 23674688
  31. Our data indicate that tPA turns on a cell signaling pathway that protects neurons from the deleterious effects of excitotoxicity. PMID: 23063501
  32. Chronic phase shifts resulted in decreased tissue-type plasminogen activator mRNA expression in the liver. PMID: 23249568
  33. The serine protease tissue plasminogen activator (tPA) and two matrix metalloproteinases, ADAMTS-4 and ADAMTS-5, were identified as Reelin cleaving enzymes. PMID: 23082219
  34. Altogether, our data provide the first evidence that tPA influences spatial memory through its preferential interaction with GluN2D subunit-containing NMDARs. PMID: 22972996
  35. activates the NF-kappaB pathway in macrophages through a signaling pathway involving annexin A2/CD11b-mediated integrin-linked kinase PMID: 22677557
  36. This study provides new evidences supporting that tPA plays a cytokine-like role in glial activation by triggering receptor-mediated intracellular signaling circuits. PMID: 22162045
  37. This study finding showed that the tPA/plasmin cascade may act downstream of ChABC to allow for synergistic sensory and motor improvement compared with each treatment alone and suggest a potential new approach to enhance functional recovery after SCI. PMID: 22016526
  38. role in the nasal mucosa reconstruction in allergic rhinitis PMID: 21339035
  39. synthetic nonadecapeptide binds to the B region and promotes the activation of plasminogen by t-PA, and then induced effective thrombolysis PMID: 21392255
  40. tPA protects white matter from stroke-induced lesions PMID: 21576385
  41. Voluntary physical activity, but not locked wheel exposure, induced a robust increase in hippocampal mature BDNF protein levels, as well as in p11 and tPA mRNA expression; and also promoted antidepressive effects and improved learning PMID: 21371535
  42. phosphodiesterase-III inhibitor prevents the hemorrhagic transformation induced by focal cerebral ischemia in mice treated with tPA PMID: 21151895
  43. Tissue plasminogen activator is required for the development of fetal alcohol syndrome in mice PMID: 21383198
  44. tPA activity is regulated reversibly in an experience-dependent manner in the adult hippocampus PMID: 20974200
  45. Data indicate that the neuroprotective effect of neuroserpin is due to inhibition of plasmin-mediated excitotoxin-induced cell death and is independent of neuroserpin's ability to inhibit tissue plasminogen activator activity. PMID: 20864675
  46. Both tissue-type PA (tPA) and urokinase-type PA (uPA) bind to NMDA-R1 and reverse this effect, thereby enhancing acetylcholine-induced tracheal contractility. PMID: 20097831
  47. these results demonstrate a new function for tPA in the central nervous system, which most likely contributes to its pleiotropic functions. PMID: 20636282
  48. Data suggest that the MSC mediated increased activation of tPA in astrocytes promotes neurite outgrowth after stroke. PMID: 20140248
  49. We suggest that AVP secretion is critically regulated by tPA-dependent facilitation of AVP release from terminals and plasminogen-dependent increase of AVP permeability across microvessels possibly via laminin degradation. PMID: 20175210
  50. Plasmin and its activators tPA and uPA play a role in the haematopoietic recovery. PMID: 19210287

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Subcellular Location Secreted, extracellular space.
Protein Families Peptidase S1 family
Database Links

KEGG: mmu:18791

STRING: 10090.ENSMUSP00000033941

UniGene: Mm.154660

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