Enterobacteria phage M13 III Monoclonal Antibody

Code CSB-MA303116A0m
Size US$210
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  • Western Blot
    Positive WB detected in: 1-3 lanes: Recombinant Enterobacteria phage M13 Attachment protein at 5ng, 10ng, 20ng; 4 lane: Enterobacteria phage M13 Attachment
    All lanes: Enterobacteria phage M13 III antibody at 1:1000
    Secondary
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 65, 55-70 KDa
    Observed band size: 65, 55-70 KDa
    Exposure time:3min
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Product Details

Full Product Name
Mouse anti-Enterobacteria phage M13 III Monoclonal antibody
Uniprot No.
Target Names
III
Alternative Names
III antibody; Attachment protein G3P antibody; Gene 3 protein antibody; G3P antibody; Minor coat protein antibody
Raised in
Mouse
Species Reactivity
Enterobacteria phage M13
Immunogen
Recombinant Enterobacteria phage M13 Attachment protein (19-424AA)
Immunogen Species
Enterobacteria phage M13
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
IgG1
Clone No.
1B8E8
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

After being immunized with the recombinant Enterobacteria phage M13 attachment protein (19-424aa), the mouse was processed to isolate B cells, which were fused with myeloma cells, resulting in hybridomas that produce the Enterobacteria phage M13 III antibody. The selected hybridomas that produce the desired antibodies were cultured in the mouse abdominal cavity, and the Enterobacteria phage M13 III monoclonal antibody was extracted from the mouse ascites. This resulting Enterobacteria phage M13 III monoclonal antibody specifically binds to Enterobacteria phage M13 species and is suitable for ELISA and WB applications. The Enterobacteria phage M13 III monoclonal antibody is purified using protein G affinity chromatography with a purity exceeding 95%.

The main function of Enterobacteria phage M13 attachment protein, also known as pIII protein, is to facilitate the attachment of the phage to the bacterial host cell during the infection process. The pIII protein interacts with specific bacterial receptors on the cell surface, allowing the phage to attach and inject its genetic material into the host cell.

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Target Background

Function
Plays essential roles both in the penetration of the viral genome into the bacterial host via pilus retraction and in the extrusion process. During the initial step of infection, G3P mediates adsorption of the phage to its primary receptor, the tip of host F-pilus. Subsequent interaction with the host entry receptor tolA induces penetration of the viral DNA into the host cytoplasm. In the extrusion process, G3P mediates the release of the membrane-anchored virion from the cell via its C-terminal domain.
Subcellular Location
Virion. Host membrane; Single-pass type I membrane protein. Note=Prior to assembly, G3P is found associated with the bacterial host inner membrane. There are about five copies of this protein per mature phage that are located on the head side of the filamentous virion.
Protein Families
Inovirus G3P protein family
Database Links

KEGG: vg:927334

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