Recombinant Human Pirin (PIR)

Code CSB-YP018032HU
MSDS
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Source Yeast
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Code CSB-EP018032HU
MSDS
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Source E.coli
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Code CSB-EP018032HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018032HU
MSDS
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Source Baculovirus
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Code CSB-MP018032HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PIR
Uniprot No.
Alternative Names
3-dioxygenase PIR; Iron binding nuclear protein; Pir; PIR_HUMAN; Pirin; Probable quercetin 2; probable quercetin 2,3-dioxygenase PIR; Probable quercetinase
Species
Homo sapiens (Human)
Expression Region
1-290
Target Protein Sequence
MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL HAEMPCSEEP AHGLQLWVNL RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGW TSFIYTISGD VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).
Gene References into Functions
  1. Data suggest that only the Fe(III) form of pirin [not the Fe(II) form] enhances affinity of binding between p65 and DNA in the pirin-p65-DNA supramolecular complex. (pirin = quercetin 2,3-dioxygenase; p65 = RELA proto-oncogene NF-kappaB subunit) PMID: 28825294
  2. Pirin regulates E-cadherin independently of Bcl3-Slug signaling. PMID: 25680527
  3. The NRF2 transcription factor binds to this element in vivo and drives the basal PIR expression. PMID: 24390086
  4. Pirin is a newly identified binding partner of EAF2/U19 capable of down-regulating EAF2/U19 protein and alleviating its inhibition of prostate cancer cell survival/proliferation. PMID: 24272884
  5. Data indicate that the R-shaped area composes the interface for pirin-NF-kappaB binding that is responsible for modulation of NF-kappaB's DNA-binding properties. PMID: 23716661
  6. pirin may have a relevant role in melanoma progression. PMID: 21514450
  7. knockdown of pirin or treatment with the small molecule inhibited melanoma cell migration PMID: 20711196
  8. This study aimed to examine genetic variations in the PIR gene by a comprehensive tagging method and its sex-specific effects on bone mineral density and osteoporotic risk. PMID: 19766747
  9. An abnormal pattern of PIR sub-cellular localization is a characteristic feature of a subset of melanomas, and suggest it may represent a marker associated with disease progression. PMID: 20089166
  10. Pirin downregulation is a feature of acute myeloid leukemia and leads to impairment of terminal myeloid differentiation. PMID: 20010624
  11. x-ray crystallography of of human pirin shows that it is an iron-binding nuclear protein and transcription cofactor PMID: 14573596
  12. First report of enzymatic activity for any member of the Pirin family which may be an important connection to their roles in transcriptional regulation. PMID: 15951572

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Pirin family
Tissue Specificity
Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
Database Links

HGNC: 30048

OMIM: 300931

KEGG: hsa:8544

STRING: 9606.ENSP00000369785

UniGene: Hs.495728

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