Human Klotho ELISA Kit

Code CSB-E13235h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name klotho
Alternative Names Kl ELISA Kit; KLOT_HUMAN ELISA Kit; Klotho peptide ELISA Kit
Abbreviation KL
Uniprot No. Q9UEF7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Developmental Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human Klotho in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 89  
Range % 86-92  
1:2 Average % 98  
Range % 91-105  
1:4 Average % 96  
Range % 92-102  
1:8 Average % 93  
Range % 86-99  
The recovery of human Klotho spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 89-98  
EDTA plasma (n=4) 97 90-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.491 2.452 2.472 2.350  
5 2.104 2.076 2.090 1.968  
2.5 1.504 1.562 1.533 1.411  
1.25 0.869 0.931 0.900 0.778  
0.625 0.494 0.507 0.501 0.379  
0.312 0.292 0.279 0.286 0.164  
0.156 0.185 0.179 0.182 0.060  
0 0.126 0.117 0.122    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Q&A and Customer Reviews


Does the kit come with the QCs included? Would it be possible to have a control sample for this kit? Sorry for the confusion, that’s what they meant for QC

Thanks for your inquiry.
The standard of this kit plays the role as quality control.
So sorry that there is no separate quality control sample to provide.

Target Data

Function May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).
Gene References into Functions
  1. sKlotho is a biomarker of renal dysfunction and a potential treatment target for renoprotection in T2 diabetes mellitus. PMID: 29509906
  2. Soluble klotho levels were inversely related to brain atrophy in alcoholic patients. PMID: 29846497
  3. The tissue klotho protein expression was associated with a lower risk and progression of malignancies. Klotho may be a protective factor against malignancies risk/progression. PMID: 29775618
  4. Serum Klotho may be a novel risk factor for atherosclerosis in maintenance hemodialysis patients PMID: 30064142
  5. a single maximal aerobic exercise session of 20 min duration induced serum levels of klotho, particularly in women PMID: 27251899
  6. Knockdown of klotho gene expression normalized IGF-1R and Wnt1 protein expressions and Akt phosphorylation in granulosa cells from patients with PCOS; it also blocked the effects of insulin on apoptosis and proliferation in granulosa cells. PMID: 28673204
  7. Schizophrenia patients, KL-VS has a selective effect on memory, with heterozygotes in CD and CS clusters performing worse than non-carriers PMID: 28673754
  8. The change in concentration of soluble alpha-klotho during the 1.5-years follow-up was an indicator of CKD progression. Renal damage associated with a reduction of alpha-klotho may involve the upregulation of plasma aldosterone. PMID: 29843135
  9. Cord blood klotho levels were inversely correlated with leptin and insulin levels at birth PMID: 29648999
  10. solubility-Klotho level was closely correlated with kidney function PMID: 29061648
  11. To evaluate the role of klotho in the pathogenesis of systemic sclerosis, its serum concentration was measured in patients compared to healthy controls, and its association with cutaneous and visceral involvement was assessed. Spearman's test showed no significant association between klotho serum levels and systemic sclerosis activity, concerning either clinical, laboratory or instrumental findings. PMID: 29320841
  12. Klotho may play an inhibiting role in lipopolysaccharide-induced inflammation injury by inhibiting NF-kappaB and Wnt signaling pathways in HK-2 cells. PMID: 29441993
  13. High klotho expression is associated with renal fibrosis and podocyte injuries. PMID: 29590173
  14. Although our study revealed higher serum ET-1 and lower serum alpha-Klotho levels in systemic sclerosis patients compared to healthy controls, there were not any significant correlations between their serum levels with severity of organ involvement. PMID: 28831601
  15. The A allele of the G395A polymorphism of Klotho, which emerges the higher levels of phosphate, may be associated with the risk of mortality in Iranian patients receiving hemodialysis. PMID: 29190606
  16. This study showed that A reduced blood alpha-Klotho level was correlated with grading of cerebral Deep White Matter Lesions and was accompanied by cognitive decline as an independent risk factor. PMID: 29154273
  17. Klotho deficiency is not just a biomarker but a contributing factor to the development of acute kidney injury. PMID: 29550818
  18. Forced expression of Klotho resulted in decline of activation of IGF-1R signaling, accompanied by decreased phosphorylation of its downstream targets, including AKT and ERK1/2. These data indicated that Klotho acts as a tumor suppressor via inhibiting IGF-1R signaling, thus suppressing the viability and promoting apoptosis in Tcell lymphoma. PMID: 28656297
  19. serum levels increase following kidney transplantation, although changes are modest compared to more significant improvements in renal function and iFGF-23 levels PMID: 28120476
  20. The functional KL-VS variant of the KLOTHO gene protects against the development of retinopathy in patients with T1 diabetes. PMID: 29247834
  21. the immunohistochemical expressions of Klotho and DNMT3a in tissues obtained from oral dysplasia and oral squamous cell carcinoma, is reported. PMID: 28303350
  22. Klotho suppresses Sox9 upregulation and intranuclear translocation. Klotho inhibits high phosphate-induced osteogenic activity in human aortic valve interstitial cells. PMID: 28332126
  23. Data suggest that serum levels of alpha-klotho and beta-klotho are down-regulated in patients with type 2 diabetes, regardless of diabetic complications. PMID: 27916483
  24. Low expression levels of Klotho were observed in DLBCL patients and cell lines. Enforced expression of Klotho could significantly induce cell apoptosis and inhibit tumor growth in DLBCL. Upregulation of Klotho resulted in declined activation of IGF-1R signaling, accompanied with decreased phosphorylation of its downstream targets, including AKT and ERK1/2. PMID: 28153033
  25. Data suggest that plasma alpha-klotho is down-regulated in subjects with type 2 diabetes as diabetic nephropathy progresses; plasma alpha-klotho level (not urinary alpha-klotho level) may be an early biomarker for predicting renal impairment in type 2 diabetes. PMID: 27037042
  26. Hmgb1 can inhibit Klotho gene expression and malignant phenotype in melanoma cells through activation of NF-kappaB signaling. PMID: 27779100
  27. patients with autosomal dominant polycystic kidney disease may potentially have different soluble alpha-Klotho production/clearance characteristics than the patients with other parenchymal renal diseases. PMID: 27733443
  28. KL and SFRP1 methylation were more predominant in nasopharyngeal tumors. PMID: 28812223
  29. The beta-Klotho gene expresses only in retina and optic nerve. The gamma-Klotho gene may regulate human lens epithelial cells' function mainly by the MAPK/ERK1/2 signaling pathway. PMID: 28095050
  30. Down-Regulation of Soluble alpha-Klotho is Associated with Reduction in Serum Irisin Levels in Chronic Obstructive Pulmonary Disease. soluble a-klotho is one possible factor involved in reduced irisin release from skeletal muscle PMID: 27140192
  31. Klotho is a co-receptor for FGF23. Together they play an important role in bone mineral and vitamin D metabolism. In chronic kidney disease, disturbances in bone metabolism increase cardiovascular risk. In the kidney, klotho induces phosphaturia. Klotho levels drop in chronic kidney disease. It inhibits vascular calcification. Review. PMID: 27118192
  32. A single copy of klotho allele KL-VS is associated with longevity, better health, increased cognition, and bigger regional brain volume. PMID: 28431289
  33. Review of Klotho function and dysfunction in mice and humans. [Review Article] PMID: 27576165
  34. The Klotho KL-VS variant is associated with an increase in the incidence of dementia in older men, in a dose-dependent fashion (intermediate for heterozygosis and highest for homozygosis). PMID: 28539162
  35. Our findings provide the first evidence that alpha-Klotho is significantly decreased in the microvasculature in systemic sclerosis skin PMID: 28183357
  36. indicates an inverse interrelationship between inflammation and Klotho in atherosclerosis PMID: 28963437
  37. Administration of soluble klotho significantly reduced obstruction-induced renal fibrosis in wild-type mice, but not in Trpc6 knockout mice, indicating that klotho and TRPC6 inhibition act in the same pathway to protect against obstruction-induced renal fibrosis. PMID: 27979597
  38. The role of Klotho protein in aging and central nervous system pathology is reviewed. PMID: 28988528
  39. These results support the notion that sialogangliosides and lipid rafts are membrane receptors for sKlotho and that the KL1 domain is sufficient for the tested biologic activities. PMID: 28442546
  40. Klotho is expressed in human dermal fibroblasts and dermal microvascular endothelial cells and is implicated in the process of angiogenesis. PMID: 27260080
  41. A factor for functioning of the brain in aged is KLOTHO gene. PMID: 26405063
  42. Data suggest Klotho attenuates ox-LDL- (oxidized low density lipoprotein)-induced oxidative stress in vascular endothelial cells via up-regulation of oxidative scavengers (lipoprotein and nitric oxide), activation of the PI3K/Akt/eNOS signaling, and down-regulation of LOX1 (lectin-like ox-LDL receptor) expression. (PI3K = phosphatidylinositol 3-Kinase; Akt = proto-oncogene c-Akt; eNOS = endothelial nitric oxide synthase) PMID: 28407763
  43. Targeting klotho may thus represent a therapeutic strategy . PMID: 27696667
  44. FGF23 affected NO synthesis and oxidative stress in human coronary artery endothelial cells are modulated by Klotho. PMID: 27448998
  45. Conversely, siRNA-mediated Klotho silencing up-regulated Egr-1, FN, and Col I expression and the p-Smad3/Smad3 ratio. Moreover, the effects of si-Klotho on Egr-1 expression were abolished by the TGF-beta1 inhibitor SB-431542. Klotho overexpression can prevent mesangial ECM production in high-glucose-treated human MCs, an effect that has been partially attributed to Egr-1 down-regulation facilitated by TGF-beta1/Smad3 s... PMID: 28411025
  46. Klotho levels were higher in acute kidney injury patients and females and lower in end stage renal disease patients than in healthy adults and patients with moderate chronic kidney disease. PMID: 28157067
  47. Genetic polymorphisms such as the G-395A polymorphism in the promoter region of the Klotho gene have been associated with the development of essential hypertension. PMID: 27215557
  48. Klotho was demonstrated to act as a potent tumor suppressor in human ovarian cancer cells. Reduced Klotho expression was detected in the specimens of patients with ovarian cancer, and overexpression of Klotho significantly inhibited cell proliferation of human ovarian cancer cells. PMID: 28259911
  49. sKlotho is increased in active acromegaly and normalises after successful treatment. PMID: 27387242
  50. Any polymorphism altering the function of klotho gene may result with stone formation. We found that there are more GG sequences of G395A gene in patients with urinary tract stone disease. That may be a polymorphism of klotho gene which results with stone formation. PMID: 26597587
  51. There is no association between the KL polymorphism and FGF23 concentration in patients undergoing long-term Hemodialysis. PMID: 27055909
  52. The rs3752472 and rs650439 single-nucleotide polymorphisms of Klotho gene are related to the risk of calcium oxalate stones in Xinjiang Uyghurpeople, and might be one of the risk factors PMID: 28116736
  53. A high expression level of KLbeta, but not KLalpha, was an independent predictive factor of short progression free survival for non-muscle invasive bladder cancer PMID: 27573985
  54. Results show that Klotho is expressed in skeletal muscle and levels are reduced by smoking. PMID: 27021580
  55. The expression levels of Klotho and autophagy are decreased in drug-resistant lung cancer cells. PMID: 27661766
  56. Overexpression of miR-199b-5p canceled the effects of atrasentan on klotho expression and apoptosis of renal tubular cells in both in vivo and in vitro. PMID: 26813039
  57. The influence of Klotho gene variants on Klotho gene expression levels in human vascular tissue and their association with cardiovascular disease and cardiovascular risk factors. PMID: 26538295
  58. This study showed that serum Klotho concentration tends to be higher in MS patients when compared to control group. PMID: 26944121
  59. Overexpression of klotho is associated with breast cancer. PMID: 26556877
  60. Klotho mRNA and protein levels were reduced in preeclamptic placentas compared with controls. -744delA single-nucleotide polymorphism was significantly associated with preeclampsia. PMID: 26972301
  61. These results suggest that free klotho mediates the FGF23-induced inhibition of 1,25VD synthesis. PMID: 26287968
  62. Data suggest that down-regulation of serum Klotho level may play role in development of atherosclerosis and endothelial dysfunction in subjects with type 1 diabetes. PMID: 26601789
  63. The data suggest that secreted Klotho protein attenuates the osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro through FGFR1/ERK signaling. PMID: 26607681
  64. Data revealed the existence of a moderating effect between Klotho and STC1, where Klotho may inhibit thyroid tumor progression by inhibiting the tumor marker level of STC1. PMID: 26531219
  65. KLOTHO G-395A polymorphism associates with reduced cognitive impairment in a sample of Chinese nonagenarians and centenarians. PMID: 26732817
  66. The -395A allele of the KLOTHO gene may be a protective genetic factor for hypertension in the Chinese population. PMID: 26634510
  67. The -395A allele carriers of the Klotho gene were correlated with lower risk of metabolic syndrome in a Chinese elderly population. PMID: 26880028
  68. Full-length, membrane-bound Klotho is not expressed in healthy or uraemic human vascular tissue. PMID: 26116633
  69. The preliminary investigation into the correlation between markers of osteo- and sarcopenia and Klotho demonstrated a decreased Klotho concentration in beta-TM patients and a higher probability of having had fragility fractures. PMID: 26460265
  70. Inhibition of mTOR signaling ameliorates vascular calcification via Klotho upregulation. PMID: 26061549
  71. Lower expression of Klotho is associated with liver cancer. PMID: 26499380
  72. MPV was significantly correlated with log(FGF21) (R=-0.167, p<0.05) and log(FGF23) (R=0.351, p<0.001) but not with log(alpha-Klotho). PMID: 25781694
  73. G-395A polymorphism of Klotho gene could increase the risk of colorectal cancer PMID: 26261651
  74. low serum Klotho levels were associated with increased epicardial fat thickness and Carotid artery intima-media thickness and with the decreased flow-mediated dilation in the study population. PMID: 26289053
  75. Results showed that KLOTHO promoter hypermethylation contributed to the Mild Cognitive Impairment (MCI) risk in Xinjiang Han Chinese but not in Xinjiang Uygur Chinese. PMID: 26197428
  76. KL methylation is a characteristic of many breast cancer cases . However, the resulting or associated perturbation in FGFR4 expression, similar to FGF19, could be utilized as a biomarker for poor prognosis PMID: 26152288
  77. Klotho deficiency activates Wnt expression and activity contributing to senescence and depletion of stem cells, klotho protein was shown to suppress Wnt-signaling transduction, and inhibit cell senescence and preserve stem cells PMID: 26346243
  78. Report reduced klotho expression in cervical adenocarcinoma and squamous cell carcinoma. PMID: 26209729
  79. KL F352V polymorphism may increase the expression of FGF23 co-receptor, KL protein and thus may decrease renal expression of 1alpha-hydroxylase, and/or stimulate 24-hydroxylase in renal transplant recipients PMID: 26022923
  80. serum alpha-Klotho is related to serum bicarbonate and proteinuria and not to renal function in chronic kidney disease PMID: 25193108
  81. Renal Klotho expression decreased significantly according to the severity of AKI, regardless of the etiology, and low expression was associated with a poor short-term outcome. PMID: 26161015
  82. serum sKlotho levels inversely correlated with age and FGF23, and were significantly reduced in patients with osteoporosis. sKlotho may serve as a biomarker of aging independent of renal function PMID: 26462468
  83. p16(INK4a) plays a previously unrecognized role in downregulating alpha-klotho expression during ageing. PMID: 25923845
  84. these findings suggest that Klotho plays a role in sustained inflammation of the lungs, which in turn may have therapeutic implications in chronic obstructive pulmonary disease. PMID: 26385922
  85. Genetic promoter of the Klotho gene shows increased methylation with age increase resulting in gene silencing in age-related cataracts. PMID: 26221880
  86. Klotho levels were lower in women under high chronic stress, and even lower in those with more depressive symptoms, than low-stress participants. PMID: 26080320
  87. Klotho inhibits apoptosis of human umbilical vein endothelial cells induced by serum from patients with secondary hyperparathyroidism. PMID: 25873020
  88. The data presented confirm alpha-Klotho expression in the kidney tubule and in the artery and provide evidence of alpha-Klotho expression across organ systems and cell types that has not previously been described in humans. PMID: 26280509
  89. role in VEGF and TGF-beta1/Smad signaling linked to pulmonary fibrosis PMID: 24894433
  90. Reduced KL expression in COPD airway epithelial cells was associated with increased oxidative stress, inflammation and apoptosis. PMID: 26201096
  91. an association between low serum klotho levels and organic GH deficiency PMID: 25198618
  92. disturbances in the FGF23-Klotho system appear to be related to the endothelial cell injuy, thus, may be involved in pathogenesis of metabolic bone disease and in cardiovascular complications. PMID: 25380886
  93. The results suggested that the cardioprotective effects of klotho were related to the attenuation of oxidative stress, and oxidative stress suppressed the transcription of klotho PMID: 26093264
  94. Kidney transplant recipients have higher serum Klotho levels and rhEPO treatment modulates these concentrations. PMID: 24760622
  95. The results of our review clearly indicate that Klotho participates in several intracellular signaling pathways, and by regulating them, Klotho is involved in aging and longevity. PMID: 25601466
  96. Klotho protein up-regulates the activity of creatine transporter CreaT (Slc6A8) by stabilizing the carrier protein in the cell membrane PMID: 25531216
  97. Reduced alpha-klotho protein expression is associated with chronic kidney disease. PMID: 25324355
  98. the role of klotho protein on the cardiovascular system and its role in the pathogenesis of hypertension thus this will be the main aspect of our review article. PMID: 26094336
  99. The C1548T polymorphism of KL gene is associated with, and may act as a risk factor for the development of prostate cancer. PMID: 25120167
  100. Klotho is silenced in a substantial subset of the tumors and restoring its expression slows growth of EOC cells and inhibits major signaling pathways. PMID: 25827069

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Involvement in disease Tumoral calcinosis, hyperphosphatemic, familial (HFTC)
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, Apical cell membrane, Single-pass type I membrane protein
Protein Families Glycosyl hydrolase 1 family, Klotho subfamily
Tissue Specificity Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chro
Database Links

HGNC: 6344

OMIM: 211900

KEGG: hsa:9365

STRING: 9606.ENSP00000369442

UniGene: Hs.524953

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