Name: Rat Klotho ELISA Kit
Brand: CUSABIO
SKU: CSB-E14958r

Product Details

Target Name

klotho

Alternative Names

KlKlotho ELISA Kit; EC 3.2.1.31) [Cleaved into: Klotho peptide] ELISA Kit

Abbreviation

Uniprot No.

Q9Z2Y9

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Developmental Biology

Assay Principle

quantitative

Measurement

Sandwich

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat Klotho ELISA Kit was designed for the quantitative measurement of Rat Klotho protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I would like to know which type of klotho is determined by this kit (Full length transmembrane protein OR secreted klotho)?

Thanks for your inquiry.
CSB-E14958r
No matter whether klotho exists in membrane form or secreted form, this kit detects klotho in soluble form. Even if it is membrane form, it can still be detected out as long as we change or close the hydrophobic domain of transmembrane region through corresponding method such as grinding and centrifuging homogenates. Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.; The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Gene References into Functions

Intermedin1-53 may attenuate vascular calcification by upregulating alpha-Klotho via the calcitonin receptor/modifying protein complex and protein kinase A signaling. PMID: 26880455
The antiaging protein klotho inhibits angiotensin II -induced cardiomyocyte hypertrophy. PMID: 26970306
The results suggested that the cardioprotective effects of klotho were related to the attenuation of oxidative stress, and oxidative stress suppressed the transcription of klotho PMID: 26093264
klotho is involved in the maintenance of baroreflex sensitivity in the brain. PMID: 24867139
Klotho gene delivery abolished the upregulation of endothelin-1 levels and the downregulation of ETB and Mn-SOD expression in kidneys of spontaneously hypertensive rats PMID: 24979306
Klotho attenuates high glucose-induced fibronectin expression and cell hypertrophy via the ERK1/2 and p38 kinase-dependent pathways. PMID: 24721634
Klotho protects the kidney against cisplatin nephrotoxicity by reduction of basolateral uptake of cisplatin by OCT2 and a direct anti-apoptotic effect independent of cisplatin uptake. PMID: 24304882
These results provide the first anatomical localization of klotho mRNA and protein in rat brain parenchyma and demonstrate that klotho levels vary during early postnatal development. PMID: 23838326
Silencing of brain klotho potentiated and expedited cold-induced elevation of blood pressure by upregulation of endothelin-1 and the subsequent activation of the sympathetic nervous system. PMID: 20086041
the therapeutic potential of soluble Klotho protein to treat age-related disorders in mice PMID: 23041151
These results suggest that the expression of Klotho is regulated by the balance between upregulation and downregulation of Wnt signaling. PMID: 22551380
The Klotho gene can attenuate the progression of hypertension and abolishes myocardial hypertrophy and myocardial fibrosis. PMID: 23225045
these studies provide novel evidence for Klotho as a key player in myelin biology, which may thus be a useful therapeutic target in efforts to protect brain myelin against age-dependent changes and promote repair in multiple sclerosis. PMID: 23365232
Aging-related kidney damage is associated with a decrease in klotho expression PMID: 20830528
Renal mRNA and protein expression of Klotho plays important an role in progression of hypertensive renal damage in spontaneously hypertensive rats. PMID: 21646815
Fosinopril up-regulates klotho mRNA in time-dependent manner, and inhibits the down-regulated expression of klotho gene induced by Ang II. PMID: 21038689
Fosinopril and losartan can exert protection against hypertensive renal damage through upregulating Klotho expression as well as reducing oxidative stress. PMID: 21311136
New insights into the pathology of age-related degeneration of dopaminergic neurons are possibly related to Klotho-mediated regulation of vitamin D. PMID: 21276773
Valsartan can improve the brain ultrastructure of spontaneously hypertensive rats by increasing the expression of Klotho. PMID: 20360647
Suggest that klotho may act as a positive regulator of parathyroid hormone expression and secretion in secondary hyperparathyroidism. PMID: 20631679
Decrease of oxidative stress is not only responsible for the improvement of renal function but also for the recovery of Klotho expression in kidney of streptozotocin-induced diabetic rats. PMID: 20625492
AST-120 increases Klotho expression, and inhibited cell senescence in the kidney of CRF rats, probably by alleviating indoxyl sulfate overload on the kidney PMID: 19955715
klotho distribution & expression in rat models of insulin resistance; no significant differences between insulin resistant and control animals; conclude that decreased klotho expression is not a general feature of rodent models of insulin resistance PMID: 19756714
Klotho is post-translationally processed and there are regulatory mechanisms for its secretion in vivo PMID: 15135068
Insulin enhances KL shedding without increasing ADAM10 and ADAM17 mRNA and protein levels PMID: 18056631
Klotho may have a broader function in the regulation of ion transport in the kidney. PMID: 19349416
Klotho may have a role in the modulation of endothelial inflammation. PMID: 19367378

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Apical cell membrane; Single-pass type I membrane protein.; [Klotho peptide]: Secreted.

Protein Families

Glycosyl hydrolase 1 family, Klotho subfamily

Tissue Specificity

Present in cortical renal tubules and the parathyroid (at protein level). Strongly expressed in kidney. Expressed at low levels in brain, lung, intestine and ovaries.

Database Links

KEGG: rno:83504

STRING: 10116.ENSRNOP00000001449

UniGene: Rn.30061

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Code	CSB-E14958r
Size	96T,5×96T,10×96T
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Rat Klotho ELISA Kit

Product Details

Citations

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Customer Reviews and Q&A

Target Background