The preparation of Recombinant Mouse Ttr protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This Ttr was expressed in E.coli at and fused with N-terminal 6xHis-SUMO tag. According to SDS-PAGE, the purity turns out to be 90%+.
Transthyretin (Ttr) is a highly conserved homotetrameric protein, mainly synthetized by the liver and the choroid plexus of brain. The carrier role of TTR is well-known. TTR is a highly conserved protein that is found in several vertebrate species. Recently, sequences homologous to TTR, known as transthyretin-like proteins (TLPs), have been found in bacteria, nematodes, and plants. In Escherichia coli and Caenorhabditis elegans TLPs form homotetramers, like TTR, although without the ability to bind T4. TTR synthesis in brain areas, other than the choroid plexus, has been a controversial subject. The presence of TTR mRNA in murine or human brains has been detected in brain areas such as the cortex, hippocampus, or cerebellum. Besides the liver and the choroid plexus, TTR synthesis has been described in several other tissues. TTR is highly transcribed and translated in the retinal pigment epithelium (RPE), a monolayer of cells that acts as blood barrier for the retina.
||Greater than 90% as determined by SDS-PAGE.
||Ttr; Transthyretin; Prealbumin
||Mus musculus (Mouse)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
||Full Length of Mature Protein
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.