The Mouse Acetylcholinesterase (AChE) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of mouse AChE in many biological fluids, including serum, plasma, cell culture supernates, and tissue homogenates. The kit has high sensitivity, excellent specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions.
This assay employs the Sandwich-ELISA technique, in which AChE in the samples or standards is sandwiched between pre-coated anti-mouse AChE and Biotin-labeled AChE antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of AChE in the sample.
AChE is a cholinergic enzyme primarily present at postsynaptic neuromuscular junctions, particularly in muscles and nerves. It is responsible for the hydrolytic degradation of acetylcholine (ACh), a naturally occurring neurotransmitter, into acetic acid and choline. During neurotransmission, ACHE functions to terminate synaptic transmission and signaling, thus blocking neighboring receptors' ACh diffusion and activation entry into T lymphocytes. It also plays a positive role in the HIV-1 replication cycle.