Mouse Acetylcholinesterase(AChE)ELISA Kit

Instructions
Code CSB-E17521m
Size 96T,5×96T,10×96T

Product Details

Target Name Acetylcholinesterase(AChE)
Alternative Names ARACHE, N-ACHE, YT, OTTHUMP00000211347|OTTHUMP00000211349|OTTHUMP00000211356|acetylcholinesterase|apoptosis-related acetylcholinesterase
Abbreviation AChE
Uniprot No. P21836
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 mU/mL-20 mU/mL
Sensitivity 0.078 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %90
Range %84-96
1:200Average %96
Range %91-104
1:400Average %95
Range %90-100
1:800Average %95
Range %88-102
Recovery
The recovery of mouse AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-101
EDTA plasma (n=4)9892-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
202.313 2.413 2.363 2.260
101.498 1.598 1.548 1.445
50.840 0.830 0.835 0.732
2.50.479 0.489 0.484 0.381
1.250.306 0.316 0.311 0.208
0.6250.197 0.207 0.202 0.099
0.3120.135 0.146 0.141 0.038
00.103 0.103 0.103  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Subcellular Location Cell junction, synapse, Secreted, Cell membrane, Peripheral membrane protein, SUBCELLULAR LOCATION: Isoform H: Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Predominates in most expressing tissues except erythrocytes where a glycophospholipid-attached form of ACHE predominates.
Database Links

KEGG: mmu:11423

STRING: 10090.ENSMUSP00000024099

UniGene: Mm.255464

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