Mouse Acetylcholinesterase(AChE)ELISA Kit

Instructions
Code CSB-E17521m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

The Mouse Acetylcholinesterase (AChE) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of mouse AChE in many biological fluids, including serum, plasma, cell culture supernates, and tissue homogenates. The kit has high sensitivity, excellent specificity, good linearity, precision low than 10%, high recovery, good lot-to-lot consistency. Get access to more details from the product instructions.

This assay employs the Sandwich-ELISA technique, in which AChE in the samples or standards is sandwiched between pre-coated anti-mouse AChE and Biotin-labeled AChE antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate. The intensity of the color is positively correlated to the amount of AChE in the sample.

AChE is a cholinergic enzyme primarily present at postsynaptic neuromuscular junctions, particularly in muscles and nerves. It is responsible for the hydrolytic degradation of acetylcholine (ACh), a naturally occurring neurotransmitter, into acetic acid and choline. During neurotransmission, ACHE functions to terminate synaptic transmission and signaling, thus blocking neighboring receptors' ACh diffusion and activation entry into T lymphocytes. It also plays a positive role in the HIV-1 replication cycle.

Target Name Acetylcholinesterase(AChE)
Alternative Names Ache ELISA Kit; Acetylcholinesterase ELISA Kit; AChE ELISA Kit; EC 3.1.1.7 ELISA Kit
Abbreviation AChE
Uniprot No. P21836
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 mU/mL-20 mU/mL
Sensitivity 0.078 mU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %90
Range %84-96
1:200Average %96
Range %91-104
1:400Average %95
Range %90-100
1:800Average %95
Range %88-102
Recovery
The recovery of mouse AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-101
EDTA plasma (n=4)9892-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
mU/mlOD1OD2AverageCorrected
202.313 2.413 2.363 2.260
101.498 1.598 1.548 1.445
50.840 0.830 0.835 0.732
2.50.479 0.489 0.484 0.381
1.250.306 0.316 0.311 0.208
0.6250.197 0.207 0.202 0.099
0.3120.135 0.146 0.141 0.038
00.103 0.103 0.103  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse AChE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled AChE antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Gene References into Functions
  1. Results indicate that direct inhibition of acetylcholinesterase activity and up-regulation of m1 muscarinic acetylcholine receptor expression in the striatum might contribute to the beneficial effects of alpha-asarone on locomotor hyperactivity in Fmr1 knock out mice. PMID: 27316341
  2. Data show that 1-month of oral treatment with beta-asarone reduces AChE, Abeta42, APP and Beclin-1 levels and alleviates some behavioral impairments by inhibiting the autophagy via regulating the PI3K/Akt/mTOR pathway in APP/PS1 transgenic mice. The results further support the exploration of beta-asarone as a possible disease-modifying agent for the treatment of Alzheimer's disease. PMID: 27737765
  3. Results indicate that adult onset hypothyroidism caused significant memory impairment and increased fear/anxiety. Moreover, the activity of both isoforms of AChE was reduced in all brain regions examined in a brain region- and isoform-specific manner. PMID: 27317840
  4. Unusually high AChE activity may be an effect marker of exposure to ethanol. The relationship between AChE and apoptosis might represent a novel mechanism of ethanol-associated neuronal injury. PMID: 28427893
  5. Findings suggested the role of DNA methylation on acetylcholinesterase transcriptional regulation and provided insight in elucidating the DNA methylation-mediated regulatory mechanism on acetylcholinesterase expression during muscle differentiation. PMID: 27021952
  6. Nerolidol-loaded nanospheres reverse memory impairment and to prevent increased ROS and TBARS levels due to amelioration of Na(+), K(+)-ATPase and AChE activities and to activation of the antioxidant enzymes, respectively. PMID: 27807596
  7. the amounts of AChE activity, AChE catalytic subunit, structure subunit PRiMA and the amount of acetylcholine, in the brain were not, significantly, altered, suggesting the role of P2Y1R in neuron could have different function as that in muscle. PMID: 27378627
  8. In P2Y1R (-/-) mice, acetylcholinesterase expression in muscle was markedly decreased. The proline-rich membrane anchor subunit was reduced by 60 %; while the collagen tail subunit was reduced by 50 %. AChE molecular forms in the muscle were not changed. PMID: 26036470
  9. Our results are discussed in the context of AChE inhibitor therapy as used in dementia. PMID: 26506622
  10. Reduction of AChE levels in prion-infected heterozygous AChE knock-out mice leads to slightly but significantly prolonged incubation time. PMID: 25853328
  11. mRNA expressions of brain specific fatty acid protein 7 (fabp-7) and phospholipase A2 group IV (pla2g4) were significantly downregulated in AChE-deficient mice. PMID: 24573602
  12. In silico studies in probing the role of kinetic and structural effects of different drugs for the reactivation of tabun-inhibited AChE. PMID: 24312449
  13. AChE is regulated in two neuronal cell lines by APP in a manner independent of the generation of sAPPalpha, sAPPbeta, and AICD. PMID: 23897820
  14. Deficiency or inhibition of acetylcholinesterase can decrease apoptosis and protect dopaminergic neurons in the neurotoxin model of Parkinson's disease. PMID: 23201480
  15. Attribute stress-inducible cognitive impairments to cholinergic-mediated induction of miR-132 and consequently suppressed synaptic ACHE. PMID: 22246100
  16. assayed the relative activities of AChE and BChE in membrane fractions and culture medium of three different neuronal cell lines, namely the neuroblastoma cell lines SH-SY5Y and NB7 and the basal forebrain cell line SN56 PMID: 23047022
  17. Long-lasting stress-inducible changes in AChE's promoter choices, identify the chromatin changes that support this long-term transcriptional memory, and reveal HDAC4 as a mediator of these effects in the hippocampus. PMID: 23236169
  18. binding of AChE to laminin-1 alters AChE activity and leads to increased neurite growth in culture. A possible mechanism of the AChE effect on neurite outgrowth is proposed due to the interaction of AChE with laminin-1. PMID: 22570738
  19. The reduction of muscle end-plate AChE activity early during the onset of STZ-induced hyperglycemia may contribute to endplate pathology and subsequent muscle weakness during diabetes. PMID: 22739110
  20. Acetylcholinesterase is associated with apoptosis in beta cells and contributes to insulin-dependent diabetes mellitus pathogenesis PMID: 22236578
  21. Report effects of K074 and pralidoxime on antioxidant response and acetylcholinesterase reactivation in malathion-poisoned mice. PMID: 21723318
  22. Brain acetylcholinesterase activity in Dst(dt-J) mutants, revealed increases in the neostriatum, the habenula-interpeduncular pathway, the cholinergic pedunculopontine nucleus and its target structures. PMID: 21978551
  23. Dietary restriction regulates brain acetylcholinesterase in female mice as a function of age. PMID: 21870149
  24. PUM2 binds to AChE mRNA and regulates AChE expression translationally at the neuromuscular synapse. PMID: 21865157
  25. Electrophysiological and ultrastructural studies were performed on phrenic nerve-hemidiaphragm preparations isolated from wild-type and acetylcholinesterase (AChE) knockout (KO) mice. PMID: 21538119
  26. Results confirm the results of previous molecular dynamics simulations, expand the view and suggest the probable reasons for the overall conformational behavior of AChE omega loop. PMID: 20919754
  27. AChE activity is present both in the primary cleft and in the secondary folds, whereas BChE activity appears to be almost absent in the primary cleft and to be concentrated in subsynaptic folds. PMID: 20805581
  28. the function and localization of AChE in mammalian systems PMID: 20153304
  29. RNA expression profiles of the muscles of AChE knockout mice compared with those of the wild-type siblings PMID: 20381477
  30. AChE may be a pro-apoptotic factor and the inhibition of AChE reduces renal ischemia-reperfusion injury. PMID: 20054652
  31. these findings identify the AChE mRNA-targeting miR-132 as a functional regulator of the brain-to-body resolution of inflammation. PMID: 20005135
  32. neuronal hypersensitivity under stress involves neuritic replacement of acetylcholinesterase-S with acetylcholinesterase-R PMID: 11799248
  33. The effects of porphyrinogenic drugs on the brain cholinergic system (Ache, Bche, and Chrm1 levels in various regions of the brain) were examined to establish a mechanism for neurological syndrome displayed in acute porphyrias. PMID: 11929041
  34. regulation of ACHE expression in developing muscle cells PMID: 12140295
  35. Inhibitors of different structure induce distinguishing conformations in the omega loop. PMID: 12196517
  36. ligand interactions of the enzyme at the peripheral anionic site PMID: 12505979
  37. Interaction with RACKq and PKCbeta II correlates with intensified fear-induced conflict behavior PMID: 12509514
  38. an analysis of the active site and conformation PMID: 12665427
  39. fluctuations of the Omega loop residues are not tightly coupled and residues in the Omega loop exhibit distinctive conformational fluctuations and therefore are likely to contribute to transient gorge enlargements in the non-liganded enzyme. PMID: 12759360
  40. Acetylcholinesterase[AChE] histochemistry revealed an abnormal distribution of AChE-positive cells in several areas of the reeler brain, including cortices; the strongest labelling was observed in cerebellum and hippocampus when compared with controls PMID: 14535959
  41. primary role for stress-induced alternative splicing of the AChE gene to elevated contextual fear and synaptic plasticity, and the AChE-R splice variant has a major role in this process. PMID: 14581933
  42. Molecular modeling studies suggest that E2020 interacts with the active-site and the peripheral anionic site in AChE PMID: 14622273
  43. Data suggest that alternative promoter usage combined with alternative splicing may lead to stress-dependent combinatorial complexity of acetylcholinesterase mRNA transcripts and their protein products. PMID: 15123727
  44. Data describe up-regulation of acetylcholinesterase activity and compensatory down-regulation of M2 muscarinic receptors in the striatal caudate putamen and nucleus accumbens of mu-opioid receptor knockout mice. PMID: 15207914
  45. biophysical diffusion reaction rate calculations of wild-type and mutant mouse acetylcholinesterase PMID: 15345536
  46. AChE is involved in regulating cell-matrix interactions in bone PMID: 15454088
  47. Impaired formation of the inner retina in an AChE knockout mouse results in degeneration of all photoreceptors. PMID: 15579149
  48. HuR interacts with the AChE 3'-UTR to regulate posttranscriptionally the expression of AChE mRNA during myogenic differentiation PMID: 15878846
  49. structural studies of active center gorge of AChe PMID: 16259971
  50. neuromuscular system exhibits a remarkable plasticity and adaptive responses to the chronic absence of AChE activity that has important consequences for the functioning of the neuromuscular junction PMID: 16274683

Show More

Hide All

Subcellular Location Cell junction, synapse, Secreted, Cell membrane, Peripheral membrane protein, SUBCELLULAR LOCATION: Isoform H: Cell membrane, Lipid-anchor, GPI-anchor, Extracellular side
Protein Families Type-B carboxylesterase/lipase family
Tissue Specificity Predominates in most expressing tissues except erythrocytes where a glycophospholipid-attached form of ACHE predominates.
Database Links

KEGG: mmu:11423

STRING: 10090.ENSMUSP00000024099

UniGene: Mm.255464

  Tel: 301-363-4651
  Email: [email protected]
  Distributors Worldwide

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1