MYC Antibody

Datasheet
Code CSB-PA407491
Former Code(s) CSB-PA875953
Size US$299
Uniprot No. P01106
Image
  • The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA407491(c-Myc Tag Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using CSB-PA407491(c-Myc Tag Antibody) at dilution 1/10, on the right is treated with synthetic peptide. (Original magnification: ×200)
  • Gel: 10%SDS-PAGE, Lysate: 2 μg, Lane: Recombinant HA tagged protein, Primary antibody: CSB-PA407491(c-Myc Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
Immunogen Synthetic peptide: C-EQKLISEEDL conjugated with KLH.
Raised in Rabbit
Species Reactivity Human,Rat
Tested Applications ELISA,WB,IHC;ELISA:1:1000-1:10000,WB:1:1000-1:5000,IHC:1:15-1:50
Relevance A c-myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A c-myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting. It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
Form Liquid
Conjugate Non-conjugated
Storage Buffer -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method Antigen affinity purification
Isotype IgG
Alias Myelocytomatosis oncogene Tag
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Target Names MYC
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis
Involvement in disease Burkitt lymphoma (BL)
Subcellular Location Nucleus, nucleoplasm, Nucleus, nucleolus
Database Links

HGNC: 7553

OMIM: 113970

KEGG: hsa:4609

STRING: 9606.ENSP00000367207

UniGene: Hs.202453

Pathway ErbB signaling pathway
Hippo signaling pathway
Jak-STAT signaling pathway
MAPK signaling pathway
PI3K-Akt signaling pathway
TGF-beta signaling pathway
Wnt signaling pathway
Cell cycle
Cellular senescence
Signaling pathways regulating pluripotency of stem cells

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