Rabbit Tumor necrosis factor α,TNFα ELISA Kit

Code CSB-E06998Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


CUSABIO's rabbit TNFα ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of TNFα concentrations in serum, plasma, tissue homogenates, and cell lysates. This assay exclusively recognizes rabbit TNFα. The quantitative sandwich ELISA technique of this kit is based on TNFα antibody-TNFα antigen interactions and an HRP colorimetric detection system to detect the levels of TNFα in samples. The intensity of the color is positively proportional to the amount of bound TNFα in the initial step.

TNFα, commonly referred to as TNF, is a pleiotropic cytokine that plays an essential role in the innate and adaptive immune systems. It is also involved in tissue regeneration, and repair, as well as the modulation of embryonic development, the sleep-wake cycle, lymph node follicle, and germinal center formation. TNF signals through TNFR1 and TNFR2, activate NF-κB and MAPK signaling pathways regulating cell proliferation, survival, differentiation, and apoptosis. Due to its pro-inflammatory properties, TNF is implicated in inflammatory diseases, such as rheumatoid arthritis, Crohn's disease, and psoriatic arthritis. Increased TNF expression has been found in various cancer types. Especially, in breast cancer, TNF is associated with enhanced tumor cell proliferation, higher malignancy grade, increased occurrence of metastasis, and generally poor prognosis for the patient.

Target Name tumor necrosis factor (TNF superfamily, member 2)
Alternative Names
TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
Abbreviation TNF
Uniprot No. P04924
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 19.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TNFα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-92  
1:2 Average % 95  
Range % 91-105  
1:4 Average % 98  
Range % 92-102  
1:8 Average % 94  
Range % 86-98  
The recovery of rabbit TNFα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 87-99  
EDTA plasma (n=4) 92 87-101  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
5000 2.905 2.899 2.902 2.789  
2500 2.366 2.356 2.361 2.248  
1250 1.639 1.604 1.622 1.509  
625 0.971 0.939 0.955 0.842  
312 0.488 0.456 0.472 0.359  
156 0.275 0.259 0.267 0.154  
78 0.179 0.171 0.175 0.062  
0 0.116 0.110 0.113    
ELISA Data Analysis ELISA Standard Curve & Curve Expert software
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Sample type: Tissue homogenate(Liver and intestine)

Sample species: Rabbit

Review: I tried the kit to determine the best working dilution (starting concentration 100mg/mL). In the case of liver the best result was obtained with 5x-10x dilution, and for intestine the best results were using undiluted sample to 2x dilution.

By Anonymous

Target Background

(From Uniprot)
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Gene References into Functions
  1. TUNEL staining was positively correlated with TNF-a protein expression. Our findings suggest that apoptosis can be induced in the vocal fold epithelium after 120min of modal intensity phonation. In contrast, shorter durations of vibration exposure do not result in apoptosis signaling. PMID: 27577014
  2. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases. PMID: 26125840
  3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
  4. The TNFalpha-evoked Cl- current. PMID: 24084720
  5. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
  6. Hypercapnia increased expression of TNFa and decreased expression of NFKB in acute lung injury models. PMID: 21158126
  7. In the early stages of myocardial ischemia, bone marrow stem cells are mobilized and home to ischemic myocardium with a concomitant increase in expression of cytokines VEGF and TNFalpha. PMID: 20624659
  8. Ammonium perchlorate can increase gene expressions of types I, III collagens, TGF-beta(1) and TNF-alpha in lung of rabbits. PMID: 20937628
  9. TNFalpha may either be directly or indirectly involved in vascular damage following an embolic stroke. Moreover, TNFalpha may mediate some of the detrimental effects of tPA on the vascular compartment PMID: 17673188

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Subcellular Location Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families Tumor necrosis factor family
Database Links

KEGG: ocu:100009088

STRING: 9986.ENSOCUP00000005787

UniGene: Ocu.1971

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