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| Code | CSB-E06998Rb |
| Size | 96T,5×96T,10×96T |
| Trial Size |  |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
CUSABIO's rabbit TNFα ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of TNFα concentrations in serum, plasma, tissue homogenates, and cell lysates. This assay exclusively recognizes rabbit TNFα. The quantitative sandwich ELISA technique of this kit is based on TNFα antibody-TNFα antigen interactions and an HRP colorimetric detection system to detect the levels of TNFα in samples. The intensity of the color is positively proportional to the amount of bound TNFα in the initial step. TNFα, commonly referred to as TNF, is a pleiotropic cytokine that plays an essential role in the innate and adaptive immune systems. It is also involved in tissue regeneration, and repair, as well as the modulation of embryonic development, the sleep-wake cycle, lymph node follicle, and germinal center formation. TNF signals through TNFR1 and TNFR2, activate NF-κB and MAPK signaling pathways regulating cell proliferation, survival, differentiation, and apoptosis. Due to its pro-inflammatory properties, TNF is implicated in inflammatory diseases, such as rheumatoid arthritis, Crohn's disease, and psoriatic arthritis. Increased TNF expression has been found in various cancer types. Especially, in breast cancer, TNF is associated with enhanced tumor cell proliferation, higher malignancy grade, increased occurrence of metastasis, and generally poor prognosis for the patient. |
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| Target Name | tumor necrosis factor (TNF superfamily, member 2) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative Names | TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a |
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| Abbreviation | TNF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Uniprot No. | P04924 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Species | Oryctolagus cuniculus (Rabbit) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample Types | serum, plasma, tissue homogenates, cell lysates | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Range | 78 pg/mL-5000 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 19.5 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Time | 1-5h | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sample Volume | 50-100ul | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Wavelength | 450 nm | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Research Area | Immunology |
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| Assay Principle | quantitative | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Measurement | Sandwich | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Precision |
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| Linearity |
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| Recovery |
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| Typical Data |
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| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Troubleshooting and FAQs |
ELISA kit FAQs | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Storage | Store at 2-8°C. Please refer to protocol. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Lead Time | 3-5 working days | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample type: Tissue homogenate(Liver and intestine)
Sample species: Rabbit
Review: I tried the kit to determine the best working dilution (starting concentration 100mg/mL). In the case of liver the best result was obtained with 5x-10x dilution, and for intestine the best results were using undiluted sample to 2x dilution.
By Anonymous
| Function (From Uniprot) |
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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| Gene References into Functions |
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| Subcellular Location | Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted. |
| Protein Families | Tumor necrosis factor family |
| Database Links |
KEGG: ocu:100009088 STRING: 9986.ENSOCUP00000005787 UniGene: Ocu.1971 |
