Rat TNF-α ELISA kit

Sample type: Tissue homogenate(PVN tissue)

Sample species: Rat

Review: The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.

Sample type: Cell culture supernatant

Sample species: Rat

Review: The TNF-α protein level in the conditioned media.#P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one-way ANOVA followed by Bonferroni\'s post hoc test. n = 3 independent experiments.

Sample type: Tissue homogenate(lung tissue)

Sample species: Rat

Review: MSCs-HO-1 decreased LPS-mediated tumor necrosis factor α (TNF-α; a) and interleukin 1β (IL-1β; b) in sera of all groups at the indicated time points. (c, d) MSCs-HO-1 decreased LPS-induced TNF-α (c) and IL-1β (d) in the lungs of all groups at the indicated time points. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus 0 day time point, and #p < 0.05 versus lenti-HO-1-GFP and MSC groups at each time point.

Sample type: Serum

Sample species: Rat

Review: Effect of GABARAP and DCs on cartilage appearance and the expression of inflammatory cytokines in serum. In vivo cartilagerepair at 4 and 8 weeks postsurgery, (a) Macroscopic appearance and (b) macroscopic scores of femoral condyles were detected. Meanwhile, serum levels of (c) IL-6, (d) TNF-α, and (e) MMP-13 were determined using ELISA kits. Values are the means ± SD (n = 12). Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. DC: differentiated chondrocytes; ELISA: enzyme-linked immunosorbent assay; GABARAP: γ-aminobutyric acid receptor-associated protein; SD: standard deviation 9Color figure can be viewed at wileyonlinelibrary.com).

Sample type: Tissue homogenate(Colorectal tissues)

Sample species: Rat

Review: Effects of BD-SNEDDS on colon levels of inflammatory cytokines and oxidative stress.

Sample type: Tissue homogenate(Liver homogenate)

Sample species: Rat

Review: Effects of MFKE on proinflammatory biomarkers in rats treated with APAP-induced liver toxicity. (A) MRNA expressions of tumor necrosis factor-alpha, interleukin-1, and inducible nitric oxide synthase.

Sample type: Cell culture supernatant

Sample species: Rat

Review: The release of TNF-α. Single asterisk refers to p < 0.05 and double asterisks refer to p < 0.01.

Sample type: Tissue homogenate(Spinal cord tissue)

Sample species: Rat

Review: The concentrations of inflammatory cytokines IL-6 (a), TNF-α (b), and IL-1β (c) and anti‐inflammatory cytokines TGF-β (d) and IL‐13 (e) were measured by ELISA in sham-operated rats (n = 6) and in SCI rats treated with melatonin (n = 6) or vehicle (n = 6). Data are presented as mean ± SEM, ***p < 0.001: SCI + Vehicle versus sham; ### p < 0.001: SCI + Melatonin versus SCI + Vehicle, and One‐way ANOVA.

Sample type: Biological fluids(Bronchoalveolar lavage fluid)

Sample species: Rat

Review: Levels of TNF-α, IL-6, IL-8, and IL-10 detected by ELISA. n =15 in each group; The measurement data were expressed by mean ± standard deviation, one-way ANOVA was used for comparison between groups and the survival rate of 0~120 h in each group was compared by Kaplan-Meier analysis; *, p < 0.05 when compared with the normal group; #, p <0.05 when compared with the ALI + PBS group; eMSCs, mesenchymal stem cell; ALI, acute lung injury; OS, oxidative stress; TNF, tumor necrosis factor; IL, interleukin; SOD, superoxide dismutase; MDA, methylenedioxyamphetamine; GSH, glutathione; BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay; HE, hematoxylin-eosin; ANOVA, analysis of variance.

Sample type: Tissue homogenate(heart tissue homogenate)

Sample species: Rat

Review: The standard was worked perfectly in my experiment but I could not get much difference in control and disease group animal serum samples.