| Code | CSB-E11987r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-96 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:4 | Average % | 101 | ||||
| Range % | 97-105 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 93-107 | |||||
| The recovery of rat TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 85 | 80-92 | ||||
| EDTA plasma (n=4) | 99 | 95-104 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This Rat TNF ELISA Kit was designed for the quantitative measurement of Rat TNF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .
Sample type: Tissue homogenate(PVN tissue)
Sample species: Rat
Review: The cytokine levels of IL-1β(F) and TNF-α(G) from the PVN tissue as measured by ELISA. n = 10–11 for each group. *P < 0.01 and **P < 0.05 versus sham groups; &P < 0.01 and #P < 0.05 versus MI + shCtrl group.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Rat
Review: The TNF-α protein level in the conditioned media.#P < 0.05 vs Control group; *P < 0.05 vs Ang II group. The statistical analyses were performed using one-way ANOVA followed by Bonferroni\'s post hoc test. n = 3 independent experiments.
By Anonymous
Sample type: Tissue homogenate(lung tissue)
Sample species: Rat
Review: MSCs-HO-1 decreased LPS-mediated tumor necrosis factor α (TNF-α; a) and interleukin 1β (IL-1β; b) in sera of all groups at the indicated time points. (c, d) MSCs-HO-1 decreased LPS-induced TNF-α (c) and IL-1β (d) in the lungs of all groups at the indicated time points. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus 0 day time point, and #p < 0.05 versus lenti-HO-1-GFP and MSC groups at each time point.
By Anonymous
Sample type: Serum
Sample species: Rat
Review: Effect of GABARAP and DCs on cartilage appearance and the expression of inflammatory cytokines in serum. In vivo cartilagerepair at 4 and 8 weeks postsurgery, (a) Macroscopic appearance and (b) macroscopic scores of femoral condyles were detected. Meanwhile, serum levels of (c) IL-6, (d) TNF-α, and (e) MMP-13 were determined using ELISA kits. Values are the means ± SD (n = 12). Bars with different letters are significantly different from each other at p < 0.05 and those with the same letter exhibit no significant difference. DC: differentiated chondrocytes; ELISA: enzyme-linked immunosorbent assay; GABARAP: γ-aminobutyric acid receptor-associated protein; SD: standard deviation 9Color figure can be viewed at wileyonlinelibrary.com).
By Anonymous
Sample type: Tissue homogenate(Colorectal tissues)
Sample species: Rat
Review: Effects of BD-SNEDDS on colon levels of inflammatory cytokines and oxidative stress.
By Anonymous
Sample type: Tissue homogenate(Liver homogenate)
Sample species: Rat
Review: Effects of MFKE on proinflammatory biomarkers in rats treated with APAP-induced liver toxicity. (A) MRNA expressions of tumor necrosis factor-alpha, interleukin-1, and inducible nitric oxide synthase.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Rat
Review: The release of TNF-α. Single asterisk refers to p < 0.05 and double asterisks refer to p < 0.01.
By Anonymous
Sample type: Tissue homogenate(Spinal cord tissue)
Sample species: Rat
Review: The concentrations of inflammatory cytokines IL-6 (a), TNF-α (b), and IL-1β (c) and anti‐inflammatory cytokines TGF-β (d) and IL‐13 (e) were measured by ELISA in sham-operated rats (n = 6) and in SCI rats treated with melatonin (n = 6) or vehicle (n = 6). Data are presented as mean ± SEM, ***p < 0.001: SCI + Vehicle versus sham; ### p < 0.001: SCI + Melatonin versus SCI + Vehicle, and One‐way ANOVA.
By Anonymous
Sample type: Biological fluids(Bronchoalveolar lavage fluid)
Sample species: Rat
Review: Levels of TNF-α, IL-6, IL-8, and IL-10 detected by ELISA. n =15 in each group; The measurement data were expressed by mean ± standard deviation, one-way ANOVA was used for comparison between groups and the survival rate of 0~120 h in each group was compared by Kaplan-Meier analysis; *, p < 0.05 when compared with the normal group; #, p <0.05 when compared with the ALI + PBS group; eMSCs, mesenchymal stem cell; ALI, acute lung injury; OS, oxidative stress; TNF, tumor necrosis factor; IL, interleukin; SOD, superoxide dismutase; MDA, methylenedioxyamphetamine; GSH, glutathione; BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay; HE, hematoxylin-eosin; ANOVA, analysis of variance.
By Anonymous
Sample type: Tissue homogenate(heart tissue homogenate)
Sample species: Rat
Review: The standard was worked perfectly in my experiment but I could not get much difference in control and disease group animal serum samples.
By Anonymous
Seeking for ELISA kits of TNF a .I am working on SD Rats and project related to High Fat Diet induced Metabolic complications.
Rat TNF-α ELISA kit -CSB-E11987r this kit can be used for estimation in BALF...fluid
Kindly answer me.
I want to know what the normal serum concentrations of TNFa in experimental rats are and the serum concentrations of TNFa in an induced freund adjuvant RA in experimental rats during 7 weeks. And I want to know the concentrations estimated in nano gramml. I want to ask about the weight loss of experimental animal rats also during an induced freund adjuvant arthritis RA during 7 weeks estimated by the gram.
We used this CSB-E11987r Rat TNF-α ELISA kit to measure normal serum/plasma samples on rats. The model with heavy inflammation could even reach hundreds of pg/ml. The results varied with different conditions of the sample and different organism models. As to the mentioned induced model, we are not so sure about the sample condition. We suggest taking the actual measurement as your real result.
As to your question about weight loss, it usually depends on your own experiment aim or the choice of your target. If you just want to test TNF-α, then it is just ok to use the form of concentration of TNF-α.
