CD4 Antibody

Code CSB-PA001453
Size US$167
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  • Western Blot analysis of COLO205 cells using CD4 Polyclonal Antibody
  • Western Blot analysis of HepG2 cells using CD4 Polyclonal Antibody
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Product Details

Uniprot No. P01730
Target Names CD4
Alternative Names CD 4 antibody; CD4 (L3T4) antibody; CD4 antibody; CD4 antigen (p55) antibody; CD4 antigen antibody; CD4 molecule antibody; CD4 receptor antibody; CD4+ Lymphocyte deficiency; included antibody; CD4_HUMAN antibody; CD4mut antibody; L3T4 antibody; Leu3 antibody; Ly-4 antibody; Lymphocyte antigen CD4 antibody; MGC165891 antibody; OTTHUMP00000238897 antibody; p55 antibody; T cell antigen T4 antibody; T cell antigen T4/LEU3 antibody; T cell differentiation antigen L3T4 antibody; T cell OKT4 deficiency; included antibody; T cell surface antigen T4/Leu 3 antibody; T cell surface antigen T4/Leu3 antibody; T cell surface glycoprotein CD4 antibody; T-cell surface antigen T4/Leu-3 antibody; T-cell surface glycoprotein CD4 antibody; W3/25 antibody; W3/25 antigen antibody
Raised in Rabbit
Species Reactivity Human,Mouse
Immunogen Synthesized peptide derived from Human CD4 around the non-phosphorylation site of S433.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule
Gene References into Functions
  1. CD4 receptor levels are very low in THP-1 differentiated cells and that this down-regulation of the virus receptor is the result of miR-221/miR-222 up-regulation during differentiation. In THP-1 cell line stably expressing a modified CD4 that is not modulated by miR-221/miR-222, productive HIV-1 infection occurs after cell differentiation. PMID: 29301198
  2. Human BD-2 and BD-3 play a positive and negative regulatory role in development and proliferation of human effector CD4+ T cells which is essential for optimal adaptive immune responses and the control of immunopathology. PMID: 30098283
  3. These findings highlight regions of cross talk between gp120 and gp41 and identify heptad repeat region 1(HR1) residues that play important roles in regulating CD4-induced conformational changes in Env. PMID: 29875245
  4. CD4(+) and CD19(+) peripheral lymphocytes of early stage AD patients exhibit mitochondrial depletion, as seen both at the level of DNA and protein PMID: 28923392
  5. Human microRNAs-221 and -222 inhibit HIV-1 entry in macrophages by targeting the CD4 viral receptor. PMID: 28978468
  6. CD4 has four ecto-domains (D1-D4), D1, D2, and D4 each contain a distinctive disulfide bond. Reduction of D2 disulfide decreases the dynamics of its surrounding beta-strands. Favorable inward collapse of structure occurs around the D2 disulfide after reduction. PMID: 29470989
  7. Our results indicate that CD4 expression and older age are adverse prognostic factors in wild-type NPM1, FLT3-ITD-negative CN-AML. PMID: 28318150
  8. We investigated the prevalence, magnitude and phenotype of CTAg-specific T cells in the blood of patients with testicular germ cell tumors (TGCTs). CD8(+) and CD4(+) T-cell responses against MAGE-A family antigens were present in 44% (20/45) of patients' samples assayed by ex vivo IFN-gamma ELISPOT. Spontaneous T-cell immunity against CTAg proteins therefore develops in many patients with testicular cancer. PMID: 28555838
  9. Depletion of the gamma2 or mu1A (AP1M1) subunits of AP-1, but not of gamma1 (AP1G1), precludes Nef-mediated lysosomal degradation of CD4. PMID: 27909244
  10. findings provide a mechanistic explanation for previous observations that dimerization-defective Nef mutants fail to down-regulate CD4 and validate the Nef dimerization interface as a target site for antiretroviral drug development PMID: 28031466
  11. Mouse leukemia cell lines that could express hCD4 and CCR5 were thus established to facilitate normal entry of HIV-1 so that a human CD4/CCR5 transgenic mice cell model can be used to investigate the transmission and pathogenesis of HIV/AIDS and potential antiviral drugs against this disease. PMID: 28028680
  12. The percentage of lamina propia CD4+LAP+ cells is increased in active ulcerative colitis, showing reduced suppressor activity due to their increased proportion of intracellular IL-17 expression. PMID: 26589955
  13. possible therapeutic targets for childhood severe asthmatics identified thru DNA microarray PMID: 25979195
  14. The study gives insights into the role of CD4 on cell membrane mechanical characteristics. PMID: 26362701
  15. A decrease of CD4(+) CD25(+) CD127(low) FoxP3(+) regulatory T cells with impaired suppressive function had been found in untreated ulcerative colitis patients. PMID: 26333292
  16. Redox shuffling of the allosteric disulfide results in previously undescribed conformational changes in CD4 that are likely important for its interaction with its protein partners. PMID: 27009680
  17. Increased levels of activated and highly susceptible HIV-1 target cells, reduced CD4 and enhanced CXCR4 cell surface expression, together with the high susceptibility to FAS-induced programmed cell death may contribute to the rapid CD4+ T cell depletion. PMID: 26452480
  18. HRB knock-down affected CD4 downregulation induced by Nef but not by HIV-1 Vpu. PMID: 26701340
  19. Increased CD4, IL-17, and COX-2 expression are associated with subclinical inflammation in malar melasma. PMID: 26381025
  20. CD4 receptor induced HIV size expansion prior to cell entry. PMID: 26432024
  21. Sustained expression of CD83 was observed when CD4+ T cells were induced by transforming growth factor-beta to differentiate into CD4+CD25+ forkhead box P3+ regulatory T (iTreg) cells. PMID: 25997495
  22. CHD in Chinese population is strongly associated with HLA-DRB1*01 and DRB1*04 haplotypes, and formation of CD4(+)CD28(null) T cells was related to HLA-DRB1*01, DRB1*04, and DRB1*15 alleles. PMID: 20842443
  23. These results lead to a model for the docking of the full AP-2 tetramer to membranes as bound to Nef, such that the cytosolic tail of CD4 is situated to interact with its binding site on Nef. PMID: 24473078
  24. This study demonstrates a null association between the CD4 C868T polymorphism and an individual's susceptibility of HIV-1 acquisition in a Chinese population. PMID: 25611551
  25. Nicotine ameliorates experimental severe acute pancreatitis via enhancing immunoregulation of CD4+ CD25+ regulatory T cells. PMID: 25742430
  26. T-cell receptor activation of human CD4(+) T cells shifts the innate toll-like receptor response from CXCL8(hi) IFN-gamma(null) to CXCL8(lo) IFN-gamma(hi). PMID: 26205220
  27. Nef domains involved in CD4 downregulation were necessary for activation of plasmacytoid dendritic Cell. PMID: 25972534
  28. Here, we describe the HIV care cascade and ART delivery supply chain to examine how mathematical modeling can provide insight into cost-effective strategies for scaling-up ART coverage in sub-Saharan Africa and help achieve universal ART coverage. PMID: 25249293
  29. Rapidly translating new science into policy is a critical component of the HIV response. Adapting and implementing the 2013 WHO treatment recommendations are necessary to prevent unnecessary illness, death, HIV transmission and costs. PMID: 25266850
  30. To augment the literature, we present a review of ART adherence interventions from 2013 to the present. We included peer-reviewed journals as well as abstracts from two key conferences. PMID: 25304006
  31. Earlier access of key populations to combination prevention interventions, combined with sustained political commitment and supportive environment for key populations, are essential for maximum impact of ART on the HIV epidemic in Vietnam. PMID: 25472886
  32. The study shows that activated CD4+ T cells can produce 1,25(OH)2D3, and that 1,25(OH)2D3 induces a 2-fold up-regulation of the VDR protein expression in activated CD4+ T cells by protecting the VDR against proteasomal degradation. PMID: 24792400
  33. Data indicate that CD4 antigen binding disrupts quaternary interactions at the HIV-1 Env trimer apex. PMID: 24931470
  34. the multi-faceted role of human SP-D against HIV-1 PMID: 25036364
  35. The effects of HIV Nef protein on down regulation of CD4 and HLA class I in patients with early and chronic HIV infection with HIV-1 subtype C is reported. PMID: 25193656
  36. The current review contributes to a better understanding of the role of AhR and its signaling pathway in CD4 helper T cell mediated inflammatory disorder. [review] PMID: 24905409
  37. Authors determined that the activation of CD4 via interaction with major histocompatibility complex class II (MHC-II) triggers cytokine expression and the differentiation of human monocytes into functional mature macrophages. PMID: 24942581
  38. HIV-1 Nef interacts with Alix in late endosomes, and this is required for efficient lysosomal targeting of CD4. PMID: 25118280
  39. Human tumor-infiltrating CD4+CD69+ T cells suppress T cell proliferation via membarene -bound TGF-beta1. PMID: 24668348
  40. These results demonstrate that aptamer-facilitated cell specific delivery of shRNA represents a novel approach for efficient RNAi delivery and is potentially to be developed for therapeutics targeting specific T cells subtypes. PMID: 25241192
  41. data show that Ag-specific CD4(+) CD25(+) CD134(+) CD39(+) T cells are highly enriched for Treg cells, form a large component of recall responses and maintain a Treg-cell-like phenotype upon in vitro expansion. PMID: 24752698
  42. Binding of the HIV-1 envelope protein gp120IIIB to the CD4/CXCR4/CCR5 heterooligomer was negligible, and the gp120-induced cytoskeletal rearrangements necessary for HIV-1 entry were prevented. PMID: 24778234
  43. Using biopsies from H. pylori-positive patients, PCR was used to quantify the expression of Foxp3 mRNA and IHC was used to semi- quantify the number of CD4+ CD25+ T cells in gastric mucosa. PMID: 24901172
  44. HIV glycoprotein 120 (gp120) requires disulfide reduction in CD4 domain 1 or 2 which impairs thioredoxin-driven CD4 dimerization PMID: 24550395
  45. this study identifies a novel mechanism of Chronic heat stress immunosuppression mediated by regulating CD4 CD25 Foxp3 Tregs. PMID: 24151582
  46. The acquisition of B cell stimulating properties by naive cord blood CD4 T cells required the STAT3-dependent expression of ICOS and IL-21. PMID: 23923047
  47. CD4 retro-translocates with oxidized intrachain disulfide bridges, and only upon proteasomal inhibition does it accumulate in the cytosol as already reduced and deglycosylated molecules. PMID: 24257748
  48. CD4(+)CD25(+)FOXP3(+) Treg levels were lower in Kawasaki disease patients than healthy subjects, and levels before treatment were lower in intravenous immunoglobulin (IVIg)-resistant patients than in IVIg-sensitive patients. PMID: 23340699
  49. The CD4(372-433) peptide fragment in the given sample undergoes some rotational averaging of anisotropic interactions; additional amino acid type-specific assignments are achieved for 10 amino acid spin systems for both CD4(372-433) and Vpu. PMID: 23863698
  50. sCD4 might be considered an important parameter for RA disease progression with potential diagnostic importance. PMID: 23700441
  51. Stable CD4 cell expression and local immune activation in the lower female genital tract may promote viral replication and genital shedding and increase the risk of sexual HIV transmission. PMID: 24006463
  52. The percentage of CD4+CD25+CD127low/- Treg and Foxp3 in portal hypertension and hypersplenism was significantly increased, suggesting that they may take part in the regulation of immune function and be related to the change of T lymphocyte subsets. PMID: 23282736
  53. We recommend immunohistochemical analyses for CD123, CD56 and CD4 inblastic plasmacytoid dendritic cell neoplasm patients, particularly in cases where the initial bone marrow study indicates normal morphology. PMID: 23470050
  54. Despite a generally poor capacity for antigen uptake compared with myeloid dendritic cell(DC)s, the high expression of CD4 on plasmacytoid DCs allows them to bind and internalize HIV-1 recombinant Env glycoprotein very efficiently. PMID: 23729440
  55. HiIV-1 Nef-mediated CD4 downregulation was impaired when the residue in the hydrophobic region in the Nef C-loop was mutated to a basic residue K/R or an acidic residue E/D or to the rigid residue P, or when M168L170, L170H171, or G172M173 was mutated. PMID: 23151229
  56. In this review, CD4-positive T cells are critical in driving the pathogenesis of myocarditis. PMID: 23692566
  57. Promoter methylation regulates SAMHD1 gene expression in human CD4+ T cells PMID: 23426363
  58. low CCR5 expression on CD4 central memory cells protects CD4 cells from direct virus infection and favors the preservation of CD4(+) T cell homeostasis PMID: 23185351
  59. HIV-1 Tat protein second exon delays fas protein-mediated apoptosis in CD4+ T lymphocytes PMID: 23364796
  60. CD4, IL-17and Foxp3 may be involved in the tumor suppression caused by host immune response, and are related with non-small cell lung cancer invasion and metastasis. PMID: 23158993
  61. Low CD4 expression is associated with non-small cell lung cancer. PMID: 23098499
  62. brain-derived HIV-1 variants with highly efficient macrophage entry adopt conformations in gp120 that simultaneously alter the way in which the Env interacts with CD4 and CCR5 PMID: 23077246
  63. CD4/CD8 double-positive T cells exert regulatory function following recognition of their target autosomal HLA class I-restricted minor histocompatibility antigen. PMID: 23225889
  64. The new antibodies complement the neutralization spectrum of potent broadly neutralizing anti-CD4 binding site (CD4bs) antibodies obtained from the same individual. PMID: 22826297
  65. sequences in gp41, the CD4 binding site, and the V2 domain all have the ability to act as global regulators of neutralization sensitivity PMID: 22933284
  66. CD4 genetic polymorphisms are associated with the susceptibility and severity of rheumatoid arthritis in Egyptian women. PMID: 21626027
  67. HLA-DR9 alleles could exert a protective effect on the recognition of citrullinated vimentin epitopes by CD4+ T cells in rheumatoid arthritis. PMID: 21769486
  68. CD4+CD25+ Tregs might relate to the inflammatory activity of both autoimmune pancreatitis and pilonidal sinus. PMID: 22466163
  69. The N/C termini and the inner domain beta-sandwich and layer 2 support the noncovalent association of gp120 trimer with the CD4. PMID: 22696649
  70. The study provides evidence for impairment of CD4(+)CD25(+) regulatory T cells -including decreased frequencies in peripheral blood and bone marrow in patients with in acquired aplastic anemia. PMID: 22797698
  71. Data indicate that the frequency of Foxp3(+)CD25(high) CD4(+)-T cells is augmented and correlated with the maintenance of a better cardiac function in indeterminate (IND) Chagas disease. PMID: 22672991
  72. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A PMID: 22384114
  73. Individuals with latent Mycobacterium tuberculosis infection harbored high frequencies of late-stage differentiated (CD45RA(-)CD27(-)) antigen-specific effector memory CD4(+) T cells that expressed PD-1. PMID: 22545156
  74. The C868T single nucleotide polymorphism in the CD4 receptor encodes an amino acid substitution of tryptophan for arginine in the third domain PMID: 21902583
  75. CD4+ CD25+FoxP3+ cells sorted from peripheral blood of juvenile idiopathic arthritis patients had statistically lower ability to suppress CD4+ CD25- cell proliferation in comparison with cells obtained from controls. PMID: 21249500
  76. Letter: report increased frequency of CD127/CD215 co-expressing CD4(+) T cells in Wegener's granulomatosis. PMID: 22640659
  77. The immunological monitoring of CD$+CD25+ Treg may be useful to predict the prognosis for patients with pancreatic cancer. PMID: 22158072
  78. The peripheral blood from 30 patients with HCV-associated chronic liver disease and 20 healthy controls by flow cytometry for the evaluation of the Treg population [CD4+CD25+ forkhead box protein 3 (Foxp3)+, were analysed. PMID: 22446965
  79. Univariate analysis with Spearman's rho showed a significant correlation between early CD4+ lymphocyte recovery and overall survival, transplant-related mortality, stem cell source and type of donor. PMID: 22337266
  80. DC-SIGN increases the affinity of HIV-1 envelope glycoprotein interaction with CD4 PMID: 22163292
  81. Data demonstrate that HIV-specific CD4 T cell responses can be used during the earliest phase of HIV infection as an immunological predictor of subsequent viral set point and disease outcome. PMID: 22378925
  82. Neutralizing antibodies to the conserved CD4 binding site exert selection pressure on the conserved CD4bs epitope of HIV-1 Env. PMID: 22419808
  83. CD4-bound conformation represents a "ground state" for the gp120 core. PMID: 22451932
  84. Absolute of CD4(+)CD25(+)FOXP3(+) regulatory T-cell count rather than its ratio in peripheral blood is related to long-term survival of renal allografts. PMID: 22310634
  85. Confirms the importance of tuberculin skin test result and baseline CD4 lymphocyte count when considering the development of tuberculosis in HIV-positive patients receiving HAART. PMID: 22283893
  86. The levels of inflammatory factors are associated with the variation of Foxp3+Tregs in COPD. PMID: 22032685
  87. Data show that production of IFN-gamma and IL-17A from duodenal biopsy culture was suppressed in hookworm-infected participants, and increased levels of CD4+CD25+Foxp3+ cells in the circulation and mucosa are associated with active celiac disease. PMID: 21949691
  88. Endocytosis of HIV-1 into plasmacytoid dendritic cells is CD4- and dynamin-dependent. PMID: 22209232
  89. Trp22 in the Vpu transmembrane domain and Gly415 in the CD4 transmembrane domain are critical for Vpu-induced targeting of CD4 to endoplasmic reticulum associated degradation. PMID: 22090097
  90. Increased CD4(+) CD69(+) CD25(-) T cells exert a critical role in hepatocellular carcinoma progression and might represent a clinically aggressive tumor phenotype. PMID: 21557772
  91. Pancreatic cancer cells inhibit CD4 T-cell proliferation and migration, induce IFNgamma production, and favor a CD69 subset expansion, suggesting that CD4 T cells play an important role in pancreatic cancer immune evasion. PMID: 21792088
  92. Demonstrate an increase in the proportion of CD4+CD25highIL-7Ralphahigh activated T cells in kidney transplant recipients, regardless of the immunosuppressive regimen. PMID: 21757642
  93. Data provide an atomic-level description of the CD4-binding site on MHC class II and reveals how CD4 recognizes highly polymorphic HLA-DR, -DP, and -DQ molecules by targeting invariant residues in their alpha2 and beta2 domains. PMID: 21900604
  94. Data shsow that the new compounds display a wide range of potency for CD4 down-modulation in CHO.CD4-YFP cells. PMID: 21800875
  95. Characterize the expression of multiple inhibitory molecules on HCV-specific CD4+ T- cells and their functional impact on CD4+ T-cell dysfunction. PMID: 21763239
  96. Suggest decreased CXCR3 expression in CD4+ T cells exposed to asbestos or derived from asbestos-exposed patients may contribute to reduced antitumor immune function. PMID: 21357438
  97. Mutant CD4 with 4 mutations is folded and more stable compared to the original CD4, but both bind gp120 with comparable affinity and are useful starting points for the design of other more complex viral entry inhibitors. PMID: 21827143
  98. CD4+ T cells switch macrophage M2-type cells in cervical tumor supernatant to M1-like macrophages and activate them to produce interleukin (IL)-12p70. PMID: 21709158
  99. The aim of this study was to evaluate regulatory T lymphocytes (Tregs) in the course of allergic contact dermatitis (ACD) and to elucidate the role of IL-10 and TGF-beta in Tregs activity. PMID: 21744325
  100. an increase in CD4+CD57+ T cells controls the capability of PB to produce the anti-tumor cytokine IFN-gamma and PB-IFN-gamma production is impaired with HCC tumor progression PMID: 21491089

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Subcellular Location Cell membrane, Single-pass type I membrane protein
Tissue Specificity Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells
Database Links

HGNC: 1678

OMIM: 186940

KEGG: hsa:920

STRING: 9606.ENSP00000011653

UniGene: Hs.631659

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