Tnf Antibody

Code CSB-PA023955ZA01MO
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Product Details

Full Product Name Rabbit anti-Mus musculus (Mouse) Tnf Polyclonal antibody
Uniprot No. P06804
Target Names Tnf
Alternative Names Tnf antibody; Tnfa antibody; Tnfsf2 antibody; Tumor necrosis factor antibody; Cachectin antibody; TNF-alpha antibody; Tumor necrosis factor ligand superfamily member 2 antibody; TNF-a) [Cleaved into: Tumor necrosis factor antibody; membrane form antibody; N-terminal fragment antibody; NTF); Intracellular domain 1 antibody; ICD1); Intracellular domain 2 antibody; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor antibody; soluble form] antibody
Raised in Rabbit
Species Reactivity Mus musculus
Immunogen Recombinant Mouse Tumor necrosis factor (Tnf), partial (57-235AA)
Immunogen Species Mus musculus (Mouse)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Made-to-order

Target Data

Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.; FUNCTION
Gene References into Functions
  1. Following Unfolded Protein Response activation during autosomal dominant retinitis pigmentosa progression, secrete TNFalpha and signal a self-destructive program to the cones, resulting in their cell death. PMID: 27750040
  2. In a hypercaloric environment, persistent elevation of microglial reactivity and consequent TNFalpha secretion induces mitochondrial stress in POMC neurons that contributes to the development of obesity. Specific disruption of the gene expressions of TNFalpha downstream signals TNFSF11A or NDUFAB1 in the mediobasal hypothalamus of diet-induced obese mice reverses mitochondrial elongation and reduces obesity. PMID: 28489068
  3. persistent stimulation with titanium particles may lead to a consistent release of TNF-alpha and IL-6 via SPHK-2 activity, which may lead to aseptic implant loosening PMID: 29728804
  4. Recognition memory improved with exercise in WT mice, was impaired in TNFR1(-/-) exercise mice, showed non-significant impairment with exercise in TNF(-/-) mice, and no changes in TNFR2(-/-) mice. In spatial learning there were exercise related improvements in WT mice, non-significant but meaningful impairments evident in TNFR1(-/-) exercise mice, modest improvement in TNF(-/-) exercise mice. PMID: 29969604
  5. In vitro mild uncoupling rescued from TNF-induced endothelial permeability, disassembly of cell contacts and VE-cadherin cleavage by the matrix metalloprotease 9 (capital EM, Cyrilliccapital EM, Cyrilliccapital ER, Cyrillic9). The uncouplers prevented TNF-induced expression of MMP9 via inhibition of NFkappaB signaling. PMID: 28131916
  6. macrophage-TNF-induced AKT/beta-catenin signalling in Lgr5(+) hair follicle stem cells has a crucial role in promoting hair follicle cycling and neogenesis after wounding PMID: 28345588
  7. Transmembrane TNF, TNFR2 and TNFR1 (indirectly) are critical for preventing inflammation during BCG-induced pleurisy in mice. PMID: 29973541
  8. findings demonstrate a new role for TNFalpha as a key regulator of neutrophil trafficking into and within lymphatic system in vivo. PMID: 28287124
  9. Our work suggested that TNF-alpha and TNF-R1 are the major contributors of TNF signaling pathway in anesthesia-induced spinal cord neurotoxicity. Targeting TNF-alpha / TNF-R1, not TNF-R2 signaling pathway may be the key component to rescue or prevent anesthesia-induced apoptotic injury in spinal cord neurons. PMID: 29802833
  10. observation from the present research work reveals that Quercetin suppressed the production of proinflammatory cytokines at different levels, such as TNF-alpha and IL-1beta, and inhibits the activation of I-kappaB phosphorylation, whereas the total content was not affected. PMID: 29322353
  11. this is the first evidence to suggest that TET2 mutations promote clonal dominance with aging by conferring TNFalpha resistance to sensitive bone marrow progenitors while also propagating such an inflammatory environment. PMID: 29195897
  12. Elevated A20 promotes TNF-induced and RIPK1-dependent intestinal epithelial cell death PMID: 30209212
  13. M. tuberculosis and TNFalpha synergise to induce necroptosis in murine fibroblasts via RIPK1-dependent mechanisms and characterized by phosphorylation of Ser345 of the MLKL necroptosis death effector. PMID: 28892415
  14. Our current study has demonstrated that in allergic airway disease (AAD) mice, intestinal dysbiosis (ID) caused increased nasal rubbing, sneezing, serum OVA specific IgE level and pro-inflammatory cytokine TNF-alpha in NALF and BALF. ID also inhibited miR-130a expression in AAD mice. Further molecular experiments indicated that miR-130a could specifically target and repress TNF-alpha mRNA expression. PMID: 29702281
  15. These data may indicate that insulin resistance in Adp(-/-) mice is likely caused by an increase in concentrations of TNFalpha and FFA via downregulation of PPARalpha. PMID: 29445073
  16. TNF-alpha is involved in cardiac PHLPP1 upregulation during reoxygenation, which is mediated by NF-kappaB transcriptional activity PMID: 29940243
  17. Lack of TNF-alpha signaling through Tnfr1 makes the mice more susceptible to acute infection but does not alter state of latency and reactivation of HSV-1. PMID: 29113822
  18. although TNFalpha does not induce osteoclastogenesis alone, it does work with RANKL to induce osteoclastic differentiation, and the NFkappaB pathway may serve an important role in this process. PMID: 29512766
  19. two different modes of necroptosis induction by TNFalpha exist which are differentially regulated by iuRIPK1 formation. Overall, this work reveals a distinct mechanism of RIPK1 activation that mediates the signaling mechanism of RDA as well as a type of necroptosis. PMID: 29891719
  20. Results demonstrate a critical role for the TRPM2 channel in Abeta42-induced microglial activation and generation of TNF-alpha: PKC/NOX-mediated generation of ROS and activation of PARP-1 are required for Abeta42-induced TRPM2 channel activation and, furthermore, the PYK2/MEK/ERK signaling pathway as a positive feedback mechanism downstream of TRPM2 channel activation facilitates further activation of PARP-1 and TRPM2 ... PMID: 29143372
  21. TNFalpha may act reciprocally with DRA, leading to the development of intestinal inflammation. PMID: 29286110
  22. TNF-alpha plays a pivotal role in the development of nonalcoholic fatty liver disease and progression to nonalcoholic steatohepatitis. PMID: 28922680
  23. Cross-fostering and conditional knockout experiments indicated that a TNF-alpha deficit in the maternal brain, rather than in the hematopoietic system, and during gestation was responsible for the low-fear offspring phenotype. PMID: 29199072
  24. In a retinitis pigmentosa mouse model, TrkC activity generates phosphorylated Erk, which upregulates glial TNF-alpha, causing selective neuronal death. PMID: 29242588
  25. genome-wide knockdown of 19 ribosomal proteins resulted in decreased IL-10 and increased TNF-alpha production. PMID: 29657255
  26. We conclude that one of the possible regulatory mechanisms of TNF in mechanical orofacial hyperalgesia involves upregulation of the nociceptor TRPV1 PMID: 29132095
  27. The work highlighted the modulatory role of miR-105 in TNF-alpha-induced epithelial-mesenchymal transition and promoting colorectal cancer metastasis. PMID: 29238068
  28. These results suggest that glucocorticoids' effects on adipose tissue immune response, both in a pro- and an anti-inflammatory manner, depend on the nutritional status. PMID: 29847081
  29. This study demonstrated that TNF-alpha genetic deletion ameliorates the amyloid phenotype of the 5XFAD mouse model of AD. 5XFAD/TNF-alpha-/- mice exhibit significantly decreased amyloid deposition and reduced levels of AbetaPP-CTFs and amyloid-beta protein. PMID: 28826177
  30. Data suggest that expression of Tnfa in adipocytes can be regulated by dietary fatty acids; here, polyunsaturated fatty acids regulate Tnfa expression via alteration in methylation of Tnfa promoter in rats fed polyunsaturated fatty acids (safflower oil versus coconut/olive oil) and in mouse adipocyte cell line incubated with polyunsaturated fatty acid (linoleic acid versus palmitic/oleic acids). PMID: 28575756
  31. a precise mechanism for attenuation of HgCl2-induced liver dysfunction by dietary luteolin via regulating Sirt1/Nrf2/TNF-alpha signaling pathway, and provide a foundation for further study of luteolin as a novel therapeutic agent against inorganic mercury poisoning. PMID: 27853236
  32. a significant increase in plasma levels of IL-2, IFN-g and TNF-a was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
  33. findings suggest that PGRN deficiency leads to excessive NF-kappaB activation in microglia and elevated TNFalpha signaling, which in turn lead to hyperexcitability of medium spiny neurons and obsessive-compulsive behavior-like behavior. PMID: 28438992
  34. findings highlight an epigenetic mechanism by which EZH2 integrates the multifaceted effects of TNFalpha signaling to promote the inflammatory response and apoptosis in colitis. PMID: 28439030
  35. It is possible that JNK and TNF-alpha commonly contribute to kidney damage by assembling a positive feedback cycle after crush syndrome, leading to increased apoptosis in the renal cortex. HMGB1 from the muscle may be the trigger. PMID: 28701229
  36. Cytokine-inducing and anti-inflammatory activity of chitosan and its low-molecular derivative. PMID: 29513410
  37. Excessive death of hepatocytes is a characteristic of liver injury. A new programmed cell death pathway has been described involving upstream death ligands such as TNF and downstream kinases such as RIPK1. PMID: 28088582
  38. Taken together, we have demonstrated a role for TNF in the development of classically activated macrophages in listeriosis PMID: 28545808
  39. Inhibition of signaling stimulated by both TNF and IL1beta synergizes with NF-kappaB inhibition in eliminating leukemic stem cells. PMID: 28039479
  40. Calyptranthes grandifolia O.Berg (Myrtaceae) ethanolic extract inhibits TNF-alpha gene expression and cytokine release in vitro PMID: 28447740
  41. Results show that interleukin 6 (IL6) promotes oval cell proliferation and liver regeneration, while tumor necrosis factor alpha (TNFalpha) and TNF receptor-1(TNFR1) do not affect this process. PMID: 27556180
  42. This study adds to the evidence that both peripheral and brain region-specific increases in tumor necrosis factor alpha lead to both sickness and depression- and anxiety disorder-relevant behavior and do so via different pathways. PMID: 27515532
  43. Lactosylceramide-Induced Phosphorylation Signaling to Group IVA Phospholipase A2 via Reactive Oxygen Species in Tumor Necrosis Factor-alpha-Treated Cells. PMID: 28444900
  44. The current study demonstrated that honey can stimulate or suppress the mRNA expression of some pro-inflammatory cytokines in mice brains. Furthermore, honey suppresses the TNF-alpha mRNA expression in the presence of T. gondii infection but it stimulates the IL-1beta and IL-6 mRNA expression. Treatment of the mice with honey reduces parasite multiplication in the brain. PMID: 27591508
  45. aerobic interval training enhanced the anti-inflammatory indices IL-10/TNF-alpha ratio and IL-15 expression in skeletal muscle in tumor-bearing mice. PMID: 27863332
  46. findings suggest that activation of Tnf-Aicda axis and co-inhibitory signals to T cells in coordination with Th1-type immunity has critical roles in the immune response against Hepatitis B virus infection PMID: 28063995
  47. Taken together, we speculate that DT-13 inhibits endothelium vascular inflammation through regulating nitric oxide production and the expression of ROS, TNFR, IL-8, MCP-1, which are associated with inflammation. PMID: 29162452
  48. TNF signalling is required for the expansion and differentiation of pathogenic IFNgamma+CD4+ T cells that promote the irreversible loss of bone marrow function. PMID: 28671989
  49. Drugs targeting XIAP and cIAP1/2 may be effective for osteosarcoma patients whose tumors express abundant RIPK1 and contain high levels of TNFalpha. PMID: 27129149
  50. Taken together, we indicated that anti-IL-6 and anti-TNF-alpha therapy prevent intestinal permeability induced by intestinal inflammation PMID: 27155817
  51. Inflammatory Osteoclasts Prime TNFalpha-Producing CD4(+) T Cells and Express CX3 CR1 PMID: 27161765
  52. TNF-alpha and the transcription factor nuclear factor kappa B (NF-kappaB) p65 subunit were both upregulated in bleomycin-induced fibrotic lung tissue. PMID: 28731277
  53. interactions between TNF-alpha and IL-6 exacerbate oxidative stress and reduce phosphorylation of eNOS, thereby contributing to coronary endothelial dysfunction in T2D mice. PMID: 29095915
  54. miR29c stimulation during influenza virus entry enhances the ability of dendritic cells to activate lymphocytes and secrete cytokines IL-6 or TNF-alpha. PMID: 28063705
  55. TNFalpha is a prominent mediator of renal CD80 induction and resultant albuminuria. PMID: 27125280
  56. NF-kB mediated upregulation of CADM1 was identified as a mechanism of TNFalpha induced migration. PMID: 26867147
  57. Here we provide evidence that the mouse fecal microbiome is shaped, in part, by sex, age and TNF production and that these effects correlate with the degree of animals' colitis PMID: 29175304
  58. In a maternal immune activation model of neurodevelopmental disorders using TNF(-/-) mice, it was observed that TNF is not required for the expression of abnormal social or anxious behaviour. This indicates that TNF does not uniquely contribute to the development of neuronal dysfunction in this model. PMID: 28093554
  59. Results provide evidence that TNFalpha reverse signalling promotes opening of T-type Ca(2+) channels along sympathetic axons, which is required for enhanced axon growth. PMID: 28100666
  60. The effect of poly(I:C) stimulation, either through endogenous transfection or exogenous addition, on inhibition of differentiation was significantly diminished in the preadipocytes of TNF-alpha knockout mice. PMID: 27311810
  61. Results provided evidence that TNF-a promoted osteoclast formation by upregulation of Blimp1 expression through PI3K/AKT pathway. PMID: 27505147
  62. Zinc metalloprotease extracted from Bothrops moojeni venom (BmooMP-alpha-I) inhibits TNF directly by promoting its degradation. PMID: 27447669
  63. This study shows that TNF-alpha is the predominant proinflammatory cytokine that induces the secretion of LRG1. LRG1 contributes to angiogenesis-coupled de novo bone formation by increasing angiogenesis and recruiting MSCs in the subchondral bone of osteoarthritis joints. PMID: 28358372
  64. In intestinal cells, TNF activates NF-kappaB, which reduces expression of the Cl(-) / HCO3(-) exchanger SLC26A3, via direct binding to the promoter region. PMID: 28823863
  65. FcgammaR engagement by anti-TNF is required for reduction of colitis in mice and development of CD206(+) macrophages. PMID: 28756234
  66. long-term PM2.5 exposure caused hepatic steatosis and dyslipidemia through triggering inflammation, which was, at least partly, dependent on iRhom2/TNF-alpha pathway in liver-resident macrophages PMID: 28965953
  67. Pretreating mice with carrageenan once a day before injecting LPS increased the levels of IL-10 by 2.5-fold and reduced TNF-alpha production by 2-fold compared with control. So, kappa/beta-carrageenan alone and in combination with LPS enhanced the cellular activity and mobility of peritoneal macrophages by increasing cell adhesion and migration compared with control. PMID: 28130856
  68. TNF/Lymphotoxin alpha/beta deficiency influences PM2.5 exposure-induced response of energy metabolism through alterations in both food intake and energy expenditure. PMID: 28917655
  69. TNF has an essential role in TLR ligand-mediated but not protease-mediated allergic airway inflammation PMID: 28758900
  70. TonEBP expression correlated with canonical osmoregulatory targets TauT/SLC6A6, SMIT/SLC5A3, and AR/AKR1B1, supporting in vitro findings that the inflammatory milieu during IDD does not interfere with TonEBP osmoregulation. In summary, whereas TonEBP participates in the proinflammatory response to TNF-alpha PMID: 28842479
  71. Increased basal transmission and plasticity of glutamate synapses that was related to increased levels of tumor necrosis factor alpha (TNFalpha). PMID: 27552480
  72. Results showed that lncRNA SeT and miR-155 mediate Tnfalpha gene dosage control by regulating the homologous pairing of the two gene alleles and ultimately regulating its biallelic pattern. PMID: 28910376
  73. Internalized CpG oligodeoxynucleotide (ODN)-conjugated PPMs (PPM-CpG) greatly enhance the induction of selected cytokines (TNF-alpha and IL-6) in RAW 264.7 cells compared to that by the soluble CpG ODN and ionic complexes. PMID: 27503807
  74. Wnt10b expression was assessed by flow cytometry under estrogen- and TNFalpha-deficient conditions PMID: 28800644
  75. Tumor necrosis factor (TNF), a cell-extrinsic potent negative regulator of hematopoietic stem cells (HSCs), was overexpressed in bone marrow niche cells from FLT3 internal tandem duplications (FLT3 ITDs) mice. PMID: 28637883
  76. conclude that TNF-alpha produced by B2 cells is a key mechanism by which B2 cells promote atherogenesis through augmenting macrophage TNF-alpha production to induce cell death and inflammation that promote plaque vulnerability PMID: 27492217
  77. robust caspase activation in NEMO-deficient cells is concomitant with RIPK3 recruitment to the apoptosis-mediating complex. PMID: 27560715
  78. findings revealed a dual mechanism of monocyte and neutrophil recruitment by T cells relying on overlapping and nonoverlapping roles for the noninducible costimulatory receptor CD28 and the inflammatory cytokine TNF PMID: 27183621
  79. MK2-mediated phosphorylation of RIPK1 serves as a checkpoint within the TNF signaling pathway that integrates cell survival and cytokine production. PMID: 28506461
  80. TLR4 activates CASPASE-8 to cleave and remove the deubiquitinase cylindromatosis (CYLD) in a TRIF- and RIPK1-dependent manner to disable necroptosis in macrophages. PMID: 27264187
  81. impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation. PMID: 28619762
  82. The elevated cytotoxicity achieved by suppressing mTOR was a result of up-regulation of NKG2D and TNF-alpha. The inhibition of STAT5 pathways via siRNA interference or a specific inhibitor eliminated the up-regulation of NKG2D and TNF-alpha in rapamycin-treated Vgamma4 gammadelta T cells. PMID: 27256566
  83. this study shows that tumor produced TNF-alpha causes an upregulation of PD-L1 expression by tumor-associated monocytes PMID: 28184968
  84. differential regulation of exocytosis by TNF-alpha involves the actin cytoskeleton and is a necessary component for priming of the 2 major neutrophil antimicrobial defense mechanisms: oxygen radical generation and release of toxic granule contents. PMID: 28096297
  85. this study shows that vagotomy can increase tumor growth and worsen survival in a murine pancreatic cancer model mediated through tumor-associated macrophages and TNFalpha PMID: 28160574
  86. Adiponectin, TNF-alpha, and LOX-1 exert complex regulatory effects on the coronary microvascular endothelial function in atherosclerotic ApoE knockout mice. PMID: 27050429
  87. PRDX6 inhibited the carcinogenesis of hepatocellular carcinoma, and the calcium-independent phospholipase A2 activity of PRDX6 promoted cancer cell death induced by TNF-alpha. PMID: 26293541
  88. Activation of CX3CR1(high)Ly6C(low) monocytes impaired motor learning and learning-related dendritic spine plasticity through tumor necrosis factor (TNF)-alpha-dependent mechanisms. PMID: 28504723
  89. Long non-coding RNA Gm4419 could up-regulate TNF-alpha expression by competitively binding miR-466l and then contribute to inflammatory damage as well as astrocyte apoptosis during cell model-traumatic brain injury. PMID: 28688761
  90. A novel role for ATF3 in the inhibition of lipopolysaccharide-induced TNF-alpha and in the impairment of monocyte and macrophage migration, is reported. PMID: 27260954
  91. Our study suggests that additionally to its local actions in the AD brain, TNF-alpha can also indirectly modulate amyloid pathology as a regulator of peripheral inflammation. PMID: 28442538
  92. cell-autonomous role of TNF on monocytes for their survival, maintenance, and function. PMID: 28330904
  93. Neutralizing low TNFalpha levels in the tumor microenvironment sensitizes breast tumor cells to paclitaxel. PMID: 27832973
  94. TNF-alpha is up-regulated in bilateral hippocampus and in ipsilateral spinal dorsal horn after peripheral nerve injury. PMID: 28123022
  95. miR-124, targeting USP2 and USP14, negatively regulates LPS-induced TNF-alpha production in mouse macrophages, suggesting miR-124 as a new therapeutic target in inflammation-related diseases PMID: 27063215
  96. XIAP antagonizes the switch from TNFalpha-induced apoptosis to necroptosis in mouse neutrophils. PMID: 27735938
  97. demonstrated a central role for macrophages, TNF-alpha, and MCP-1 in the pathogenesis of respiratory syncytial virus-induced exacerbation of airway hyperresponsiveness and inflammation in mice with allergic airways disease PMID: 27036916
  98. These experiments reveal an essential role for TNF activation of NF-kappaB to promote the survival and development of single positive T cells in the thymus. PMID: 27432943
  99. In SOD1(G93A) spinal cords, we verified a strict correlation in the expression of the TNFalpha, TNFR1 and GDNF triad at different stages of disease progression. Yet, ablation of TNFR1 completely abolished GDNF rises in both SOD1(G93A) astrocytes and spinal cords, a condition that accelerated motor neuron degeneration and disease progression PMID: 27288458
  100. mature dendritic cell derived exosomes increase endothelial inflammation and atherosclerosis via membrane TNF-alpha mediated NF-kappaB pathway. PMID: 27515767

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Subcellular Location Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted
Protein Families Tumor necrosis factor family
Database Links

KEGG: mmu:21926

STRING: 10090.ENSMUSP00000025263

UniGene: Mm.1293

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