All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Raw Material For IVD Kit
All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Raw Material For IVD Kit

Human Tumor necrosis factor α,TNF-α ELISA KIT

Citations (123) Protocol
Code CSB-E04740h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

Human TNF-α ELISA Kit is designed to quantify human TNF-α levels in the serum, plasma, cell culture supernates, tissue homogenates, or cell lysates. It quantitates human TNF-α with 1.95 pg/mL sensitivity and shows excellent specificity for human TNF-α. This human TNF-α ELISA kit includes both the capture and the detection antibodies for TNF-α as well as avidin conjugated Horseradish Peroxidase (HRP) and TMB substrate solution, which are the reagents used in color development step. The produced color develops in proportion to the amount of TNF-α in the sample. The color development is stopped and the color intensity is measured using a microplate reader.This ELISA kit has been cited in 95 publications.

TNF-α is an inflammatory cytokine generated by macrophages/monocytes during acute inflammation. It contributes to a wide range of signaling events within cells, resulting in necrosis or apoptosis. It also plays an important role in the resistance to infection and malignancies. TNF-α binds to two different receptors TNFR1 and TNFR2, activating a downstream signal cascade, including NF-κB and MAPK pathways, evoking a variety of cellular responses, such as inflammation, host defense, cell survival, differentiation, and proliferation. Dysregulation of TNF-α signaling is related to chronic inflammation and autoimmune diseases. TNF-α inhibitors have been successfully produced and employed in the clinical treatment of autoimmune disorders such as Crohn's disease (CD) and RA due to TNF-α participation in the pathogenesis of autoimmune diseases.
Target Name tumor necrosis factor (TNF superfamily, member 2)
Alternative Names
APC1 ELISA Kit; APC1 protein ELISA Kit; Cachectin ELISA Kit; DIF ELISA Kit; Differentiation inducing factor ELISA Kit; Macrophage cytotoxic factor ELISA Kit; Tnf ELISA Kit; TNF superfamily member 2 ELISA Kit; TNF superfamily, member 2 ELISA Kit; TNF, macrophage derived ELISA Kit; TNF, monocyte derived ELISA Kit; TNF-a ELISA Kit; TNF-alpha ELISA Kit; TNFA ELISA Kit; TNFA_HUMAN ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor (TNF superfamily member 2) ELISA Kit; Tumor necrosis factor alpha ELISA Kit; Tumor necrosis factor ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; Tumor Necrosis Factor, Membrane Form ELISA Kit; Tumor necrosis factor, soluble form ELISA Kit
Abbreviation TNF
Uniprot No. P01375
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 96  
Range % 89-99  
1:2 Average % 90  
Range % 85-95  
1:4 Average % 97  
Range % 88-102  
1:8 Average % 92  
Range % 85-100  
Recovery
The recovery of human TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 94-101  
EDTA plasma (n=4) 96 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.607 2.705 2.656 2.482  
250 2.296 2.307 2.302 2.128  
125 1.642 1.624 1.633 1.459  
62.5 0.912 0.940 0.926 0.752  
31.2 0.489 0.504 0.497 0.323  
15.6 0.317 0.328 0.323 0.149  
7.8 0.282 0.271 0.277 0.103  
0 0.172 0.175 0.174 　  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Related Products

TNF antibodies

TNF Proteins

See all products about TNF >>

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 5 reviews

Submit a Review here

Sample type: Serum

Sample species: Human

Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, [email protected] concentration was 1.0 mg/mL, and incubation time was 120 minutes.

By Anonymous

Sample type: Serum

Sample species: Human

Review: Concentrations of IL-6 and TNF-α in [email protected] group maintain at normal level when the incubation time increases from 30 minutes to 180 minutes. Concentrations of IL-6 and TNF-α in [email protected] group rise above the normal level with prolonged incubation time.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Review: Same trend was observed in the concentrations of IL-1α, IL-6, IL-8 and TNF-α in cell culture supernatant.

By Anonymous

Sample type: Serum

Sample species: Human

Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodies for 24 hours.

By Anonymous

Sample type: Cell culture supernatant

Sample species: Human

Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α.

By Anonymous

Read more reviews >>

Target Background

Function
(From Uniprot)
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Gene References into Functions
  1. Genetic predisposition to rheumatoid arthritis in Russian population in the Republic of Karelia is associated with the presence of the GG TNF-alpha genotype. PMID: 30225702
  2. Treatment with 30 microg/ml curcumin also significantly diminishes the protein production of TNFalpha in Behcet's disease patients (p < .01) and healthy controls (p < .05) M1 macrophages. PMID: 29806793
  3. the protective role of the A allele in TNF-alpha 238A/G but not TNF-alpha 308A/G against the occurrence of juvenile idiopathic arthritis in the Caucasian population (Meta-Analysis) PMID: 30412082
  4. TNF-alpha expressed regionally in Epicardial Adipose Tissue may exert potent effects on the progression of coronary atherosclerosis PMID: 28931782
  5. The effect of the pro-inflammatory diet on concentrations of TNF-alpha was more pronounced in pregnant women reporting higher levels of stress. PMID: 30200631
  6. TNF-alpha -308G/A polymorphism is not associated with the risk to develop of bullous pemphigoid and alopecia areata in our Iranian cohort PMID: 29843231
  7. data support that only the promoter single-nucleotide polymorphism (SNP) rs1800629 within the TNF-alpha gene is associated with increased risk for developing Graves' disease (GD), especially in European population. Future large-scale studies are required to validate the associations between TNF-alpha gene and GD. PMID: 29440561
  8. We studied 173 polymorphisms to establish an association with the response to anti-TNF drugs in patients with moderate-to-severe plaque psoriasis (N=144). PMID: 27670765
  9. The SNP rs4819554 in the promoter region of IL17RA significantly influences the response to anti-TNF drugs at week 12. PMID: 27670766
  10. TNF-alpha promoter gene polymorphisms and/or haplotypes are risk factors of Nephrotic syndrome and resistance to steroid among Egyptian children PMID: 28803697
  11. The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for nasal polyposis in the north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. PMID: 30003390
  12. results suggest, the interplay of pro-inflammatory cytokines IFN-gamma derived from CD4+T lymphocytes and TNF-alpha from CD14+ cells has no direct additive impact on parasite replication but induces IL-4 production. PMID: 29953494
  13. The single nucleotide polymorphisms rs361525, rs1800629, and rs17999645 of tumor necrosis factor-alpha were significantly correlated with the diagnosis of cervical cancer PMID: 29940817
  14. Rs11541076 in IRAK3, a negative regulator of TLR signalling, as a predictor of anti-TNF treatment response. PMID: 27698401
  15. In this Brazilian population, TNF and IL17 gene polymorphisms responsible for the expression of important inflammatory cytokines were associated with overall spondyloarthritis and, specifically, with ankylosing spondylitis and psoriatic arthritis, regardless of gender and HLA-B27 PMID: 29849482
  16. by restraining TNFR1 at the cell surface via sialylation, ST6Gal-I acts as a functional switch to divert signaling toward survival. These collective findings point to a novel glycosylation-dependent mechanism that regulates the cellular response to TNF and may promote cancer cell survival within TNF-rich tumor microenvironments. PMID: 29233887
  17. The TNF*A allele confer susceptibility to AIH in the Tunisian patients and is associated with increased production of TNF-alpha. Anti-TNF antibodies could be an alternative to the use of corticotherapy and may avoid the exacerbated immune response in Autoimmune hepatitis. PMID: 29845365
  18. Impairment in TNF, IL-1beta, and IL-17 production upon stimulation with mycobacterial antigens may contribute to the increased susceptibility to M. tuberculosis infection observed in HTLV-1 infected individuals. PMID: 29523325
  19. LL was significantly negatively correlated with PGC-1alpha, TNF-alpha, and IL-6 mRNA expressions. PGC-1alpha mRNA expression levels in paraspinal muscles may be affected by lumbar kyphosis. PMID: 30233161
  20. TNF-alpha-308G>A polymorphism affects the overall survival of cancer patients and is a potential therapeutic target for cancer. PMID: 30407345
  21. Many inflammatory pathologies are now recognized to be driven by aberrant TNF-induced cell death, which, in most circumstances, depends on the kinase Receptor-interacting serine/threonine-protein kinase 1 (RIPK1). [review] PMID: 29217118
  22. High TNF-alpha expression is associated with retinopathy of prematurity. PMID: 29274846
  23. Tumour necrosis factor-alpha selectively reduces BMPR-II transcription and mediates post-translational BMPR-II cleavage via the sheddases, ADAM10 and ADAM17 in pulmonary artery smooth muscle cells. PMID: 28084316
  24. Polymorphisms of IL-1betab and TNF-a are not a risk of ICC, but an individual with O. viverrini infection has an effect on all genotypes of the TNF-alpha gene that might promote intrahepatic cholangiocarcinoma . Primary prevention of intrahepatic cholangiocarcinoma in high-risk areas is based on efforts to reduce O. viverrini infection. PMID: 30139338
  25. In placenta, when gestational age was controlled for, partial correlation revealed a significant positive correlation between TNF-alpha and MMP-9 only in the second trimester. PMID: 28820024
  26. Study shows that in human endometrial stromal cells, high tumor necrosis factor levels negatively affect the insulin action through decreased adiponectin signaling and glucose transporter type 4 protein. This could explain the failures observed in endometrial function of obese women with polycystic ovary syndrome. PMID: 28946816
  27. Three single nucleotide polymorphisms (SNPs) within P2X4R and two SNPs within CAMKK2 influenced concentrations of TNFalpha in peripheral blood mononuclear cells, but these SNP did not associate with risk for HIV-associated sensory neuropathy in South Africans. PMID: 29428485
  28. Results indicated that the proinflammatory cytokine TNF-alpha impairs endothelial tight junctions and promotes monocyte-endothelial cell adhesion by upregulating beta-site amyloid precursor protein enzyme 1 expression through activating PKC signaling and sequentially cleaving alpha-2, 6-sialic acid transferase 1. PMID: 28091531
  29. These findings reveal that PrP enhances the responses to TNF-alpha, promoting proinflammatory cytokine production, which may contribute to inflammation and tumorigenesis. PMID: 28900035
  30. This study found that the protein and mRNA expression levels of the cytokines TNF-alpha is significantly increased. PMID: 28476335
  31. Taking together, these results suggest that Wnt/beta-catenin signal pathway activation-dependent up-regulation of syncytin-1 contributes to the pro-inflammatory factor TNF-alpha-enhanced fusion between oral squamous cell carcinoma cells and endothelial cells. PMID: 28112190
  32. Data suggest that, in children with pediatric obesity, lifestyle weight-loss intervention results in down-regulation of serum cardiotrophin-1 (CTF1), interleukin-6 (IL6), and tumor necrosis factor-alpha (TNFA); expression of CTF1, IL6, and TNFA is also down-regulated in peripheral blood mononuclear cells after improvement in adiposity, body mass index, and waist-hip ratio. PMID: 28749076
  33. TNFalpha differently regulated the levels of PPARalpha, LXRalpha, and LXRbeta binding to the apoA-I gene promoter in THP-1 cells. Obtained results suggest a novel tissue-specific mechanism of the TNFalpha-mediated regulation of apoA-I gene in monocytes and macrophages and show that endogenous ApoA-I might be positively regulated in macrophage during inflammation. PMID: 29442267
  34. this is the first evidence to suggest that TET2 mutations promote clonal dominance with aging by conferring TNFalpha resistance to sensitive bone marrow progenitors while also propagating such an inflammatory environment. PMID: 29195897
  35. Anti-rotavirus effect of TNF-alpha was achieved by NFkappaB-regulated genes via the activation of classical nuclear factor kappaB (NF-kappaB) signaling. PMID: 29859235
  36. Results revealed that heterozygous genotype GA of TNF-alpha-238 (rs 361525) SNP significantly increased the risk of adverse-outcome (mortality rate), regardless of organ dysfunction or severity of sepsis. PMID: 29978383
  37. in the patients with primary depression, depressive symptoms were associated with TNF-alpha PMID: 30148175
  38. Addition of TNFalpha to podocytes causes CD80 upregulation, actin reorganization and podocyte injury. PMID: 29022109
  39. The results of the study suggest that the levels of C-reactive protein and tumor necrosis factor-alpha are important diagnostic markers of inflammation in patients with chronic pancreatitis and type 2 diabetes mellitus. PMID: 30280549
  40. TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
  41. the results of the present study suggested that overexpression of KLF15 in Eahy926 cells exhibited a protective effect against TNFalpha induced dysfunction via activation of Nrf2 signaling and inhibition of nuclear factor kappaB signaling. PMID: 29956764
  42. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
  43. Elevated A20 promotes TNF-induced and RIPK1-dependent intestinal epithelial cell death PMID: 30209212
  44. TNF-alpha GG genotype at -238 and GG haplotype at positions -308 and -238 were associated with Kawasaki disease in an Iranian population. PMID: 27455075
  45. We have shown that a TNFalpha gene polymorphism, rs1800629, is highly significantly associated with postmenopausal osteoporosis and BMD in the female Han Chinese population PMID: 29481288
  46. allele -308 A TNF-alpha may have a role in progression of rheumatoid arthritis in a North Indian population PMID: 28748515
  47. ATF3 mediates the inhibitory action of TNF-alpha on osteoblast differentiation and that the TNF-alpha-activated JNK pathway is responsible for the induction of Atf3 expression. PMID: 29605296
  48. TNF-alpha, DKK1, and OPG have roles in pathogenesis of knee osteoarthritis PMID: 28676900
  49. Btk acts in the TLR7/8 pathway and mediates Ser-536 phosphorylation of p65 RelA and subsequent nuclear entry in primary human macrophages. PMID: 29567473
  50. Results indicated that prolonged tumor necrosis factor (TNFalpha) exposure could have detrimental consequences to endothelial cells by causing senescence and, therefore, chronically increased TNFalpha levels might possibly contribute to the pathology of chronic inflammatory diseases by driving premature endothelial senescence. PMID: 28045034

Show More

Hide All
Involvement in disease Psoriatic arthritis (PSORAS)
Subcellular Location Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Protein Families Tumor necrosis factor family
Database Links

HGNC: 11892

OMIM: 191160

KEGG: hsa:7124

STRING: 9606.ENSP00000398698

UniGene: Hs.241570
Promotions
Contact Us

  Email: [email protected]
  Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Related Articles
TWIK2 channel mediates inflammation, study finds Osteoprotegerin (OPG): What is Its Potential as a Biomarker or Therapeutic Target? Histones and Their Roles in Diseases Multiple Myeloma, One of High-Risk Factors for COVID-19 The True Face of Tumor Suppressor Gene p53 Two Types of Macrophages: M1 and M2 Macrophages An Overview of Cell Death: Necrosis and Apoptosis TNFRSF9: A Potent Immune-Stimulator in Cancer TNFR1: A Multifaceted Molecule in Cancer, Another Promising Therapeutic Target for COVID-19 Cellular Senescence and Its Markers TFPI: a Natural Anticoagulant Protein, Future Therapies for Hemophilia Chemokine Receptor CCR4: the Key Role of CCR4+Treg Cells in Cancer Immunotherapy DT3C, a Powerful Tool for ADC Drug Internalization In Vitro CLDN6: A "New Favorite" in Targeted Cancer Therapy after CLDN 18.2! Intestinal Mucosal Barrier Bodyguard --ALPi GPRC5D: Newly Efficient GPRC5D CAR-T or Bispecific Antibody Therapy to Fight Multiple Myeloma (MM) Dual Role of TNFRSF14 in Immunology Learn to Distinguish Moles from Melanomas With the High Potential in Cancer Immunotherapy, CD40 Is Triggering Attention CD14-- A Membrane Receptor for Bacterial Lipopolysaccharides Surface Markers That Help You Identify Lymphocytes Cell Differentiation Determines the Fate of Cells CAR-T Cell Therapy- An Useful Treatment To Cancer CLDN4: A Potential Prognostic Indicator and Therapeutic Target in Tumors！ Differentiation of Th17 Cells The Overview of Fc Receptors- Classification, Related Signaling Pathways and Application Surface Markers of Mesenchymal Stem Cells Stem Cell-What a Miraculous Resource in Human Body IL-6-Do You Really Know That? Nanobody, what a Powerful Novel Antibody! CD147, a New Target of SARS-CoV-2 Invasion LPS/TLR4 Signal Transduction Pathway What You Have to Know about Cytokine Storm and Virus Infection Toll-like Receptors--The Eye of Innate Immunity Human Leukocyte Antigen: Distinguish between Friend and Foe What Are Immune Checkpoints? What Is Immune Checkpoint Therapy? What Are Immune Checkpoint Inhibitors? What Are Immune Checkpoint Proteins? Focus on Emerging Therapeutic Targets BCMA What Are Immune Checkpoint Pathways? CD20 Monoclonal Antibodies Drugs: There is an Explosion of New mAbs Against CD20! Tumor Necrosis Factor Receptor Superfamily and Apoptosis VSIG4: a Prominent B7 Family Costimulatory Molecule or Complement Receptor in Cancers and Inflammatory Diseases! Monoclonal Antibody Drugs Overview SLC39A6/ZIP6: a Key Member of Zinc Transporters, a Novel Targeting Antigen of ADC! What You Have To Know about Lung Cancer The Overview of Hippo Signaling Pathway How do Th1 and Th2 Cells Differentiate? Identify Treg Cells. You Can Do This. What A Paradoxical TNF Signaling Pathway-Based on Different Receptors Don't Let Hepatocellular Carcinoma What Are the Effects of Abnormalities in the ALK Gene Mesothelin: A Rising Star of Cancer Immunotherapy Does TNF Superfamily Inhibit Cancer or Promote Its Development? An Overview of CD226 and Its Promising Role in Cancer Immunotherapy CD Antigens - From Leucocytes Surface Markers to Diagnostic Markers for Cancer Latest Changes in Journal Impact Factor Surface Markers of Natural Killer Cells Mucin-17/MUC17: An Unexpected Target for Tumor Immune Therapy, Notably Gastric Cancer! BMTP-11 found to delay progression of osteosarcoma The Overview of Chemokine The Overview of Colony Stimulating Factors Do You Really Know Neurons and Glia Cells in Nerve System? Apoptosis mediated by death receptor PD-1/PD-L1, What a Powerful Immune Checkpoint The Memory thief - Alzheimer's disease The Overview of Growth Factor Tumor Necrosis Factor (TNF) The Overview of Interferon Tumor markers The Overview of Interleukin The Overview of Organelles The Overview of Autophagy The overview of Wnt signaling pathway The overview of TGF-β signaling pathway Bispecific Antibody Overview: From Definition to Application IGFL1: A Novel Secretory Protein of IGF-Like Family Presents a High Cancer-Promoting Potential! ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments
Related pathways
MAPK signaling pathway mTOR signaling pathway TGF-beta signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis Necroptosis Antigen processing and presentation Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Toll-like receptor signaling pathway Adipocytokine signaling pathway AGE-RAGE signaling pathway in diabetic complications Inflammatory Pain
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Email
[email protected]
Address
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2023 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1