Code | CSB-E04740h |
Size | 96T,5×96T,10×96T |
Trial Size | ![]() |
Have Questions? | Leave a Message or Start an on-line Chat |
Description |
Human TNF-α ELISA Kit is designed to quantify human TNF-α levels in the serum, plasma, cell culture supernates, tissue homogenates, or cell lysates. It quantitates human TNF-α with 1.95 pg/mL sensitivity and shows excellent specificity for human TNF-α. This human TNF-α ELISA kit includes both the capture and the detection antibodies for TNF-α as well as avidin conjugated Horseradish Peroxidase (HRP) and TMB substrate solution, which are the reagents used in color development step. The produced color develops in proportion to the amount of TNF-α in the sample. The color development is stopped and the color intensity is measured using a microplate reader.This ELISA kit has been cited in 95 publications. TNF-α is an inflammatory cytokine generated by macrophages/monocytes during acute inflammation. It contributes to a wide range of signaling events within cells, resulting in necrosis or apoptosis. It also plays an important role in the resistance to infection and malignancies. TNF-α binds to two different receptors TNFR1 and TNFR2, activating a downstream signal cascade, including NF-κB and MAPK pathways, evoking a variety of cellular responses, such as inflammation, host defense, cell survival, differentiation, and proliferation. Dysregulation of TNF-α signaling is related to chronic inflammation and autoimmune diseases. TNF-α inhibitors have been successfully produced and employed in the clinical treatment of autoimmune disorders such as Crohn's disease (CD) and RA due to TNF-α participation in the pathogenesis of autoimmune diseases. |
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Target Name | tumor necrosis factor (TNF superfamily, member 2) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Alternative Names | APC1 ELISA Kit; APC1 protein ELISA Kit; Cachectin ELISA Kit; DIF ELISA Kit; Differentiation inducing factor ELISA Kit; Macrophage cytotoxic factor ELISA Kit; Tnf ELISA Kit; TNF superfamily member 2 ELISA Kit; TNF superfamily, member 2 ELISA Kit; TNF, macrophage derived ELISA Kit; TNF, monocyte derived ELISA Kit; TNF-a ELISA Kit; TNF-alpha ELISA Kit; TNFA ELISA Kit; TNFA_HUMAN ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor (TNF superfamily member 2) ELISA Kit; Tumor necrosis factor alpha ELISA Kit; Tumor necrosis factor ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; Tumor Necrosis Factor, Membrane Form ELISA Kit; Tumor necrosis factor, soluble form ELISA Kit |
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Abbreviation | TNF | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Uniprot No. | P01375 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Species | Homo sapiens (Human) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Types | serum, plasma, cell culture supernates, tissue homogenates, cell lysates | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Range | 7.8 pg/mL-500 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sensitivity | 1.95 pg/mL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Assay Time | 1-5h | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sample Volume | 50-100ul | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detection Wavelength | 450 nm | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Area | Immunology |
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Assay Principle | quantitative | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Measurement | Sandwich | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Precision |
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Linearity |
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Recovery |
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Typical Data |
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ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Troubleshooting and FAQs |
ELISA kit FAQs | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Storage | Store at 2-8°C. Please refer to protocol. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Lead Time | 3-5 working days |
Sample type: Serum
Sample species: Human
Review: Cell viability and cytotoxicity of PBMCs (e), IL-6 (f) and TNF-α(g) secretion levels with different incubation time. Temperature was 37.5 °C, [email protected] concentration was 1.0 mg/mL, and incubation time was 120 minutes.
By Anonymous
Sample type: Serum
Sample species: Human
Review: Concentrations of IL-6 and TNF-α in [email protected] group maintain at normal level when the incubation time increases from 30 minutes to 180 minutes. Concentrations of IL-6 and TNF-α in [email protected] group rise above the normal level with prolonged incubation time.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: Same trend was observed in the concentrations of IL-1α, IL-6, IL-8 and TNF-α in cell culture supernatant.
By Anonymous
Sample type: Serum
Sample species: Human
Review: ELISA was used to analyze cytokines (TNF-α, IFN-γ, IL-2 and IL-6) expression after Jurkat and P116 cells were stimulated with anti-CD3/CD28 antibodies for 24 hours.
By Anonymous
Sample type: Cell culture supernatant
Sample species: Human
Review: The ELISA data indicate that Lnc-DC silencing significantly decreased the concentration of TNF-α.
By Anonymous
Function (From Uniprot) |
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
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Gene References into Functions |
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Involvement in disease | Psoriatic arthritis (PSORAS) |
Subcellular Location | Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted. |
Protein Families | Tumor necrosis factor family |
Database Links |
HGNC: 11892 OMIM: 191160 KEGG: hsa:7124 STRING: 9606.ENSP00000398698 UniGene: Hs.241570 |