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CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜ACE2｜TMPRSS2｜Exosome Isolation Kits
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CLDN18.2｜CD20｜Proteinase K｜EGFR｜PD-L1｜CD276｜ACE2｜TMPRSS2｜Exosome Isolation Kits
| Code | CSB-AP002161HU |
| Size |
$1309Purchase it in Cusabio online store (only available for customers from the US) |
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| Description |
The recombinant human tumor necrosis factor protein (TNF) was generated by the expression of a DNA fragment encoding 77-23aa of human TNF in the E.coli cells. The partial-length protein reached up to 98% in purity, which was measured through SDS-PAGE and HPLC analysis. It was also verified to be fully biologically active through a cytotoxicity assay using murine L929 cells (ED50 <0.05 ng/ml, specific activity >2.0x107 IU/mg). The endotoxin of this TNF is less than 1.0 EU/μg as determined by the LAL method. In-stock TNF proteins are offered now. Proteins carrying custom tags are also available. This recombinant TNF protein may find uses in the specific antibody synthesis or the cancer studies. TNF, also called TNF-α, is a pro-inflammatory cytokine and a key mediator of inflammation. It exerts pro-inflammatory effects via TNF-α/TNFR signaling. When a person gets injured or contracts bacterial or viral infections, TNF triggers inflammation to protects people against infections and help the injury to heal. Whereas overexpressed TNF causes excess inflammation and even probably leads to autoimmune diseases. Inflammatory autoimmune conditions such as psoriatic arthritis, rheumatoid arthritis (RA), Crohn’s disease, among others are related to TNF. Anti-TNF therapy has been clinically put into use to control autoimmune diseases such as RA. Studies have shown that TNF can kill certain tumor cells. |
| Purity | >98% as determined by SDS-PAGE and HPLC. |
| Endotoxin | Less than 1.0 EU/μg as determined by LAL method. |
| Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0x107 IU/mg in the presence of actinomycin D. |
| Target Names | TNF |
| Uniprot No. | P01375 |
| Research Area | Cancer |
| Alternative Names | APC1; APC1 protein; Cachectin; DIF; Differentiation inducing factor; Macrophage cytotoxic factor; Tnf; TNF superfamily member 2; TNF superfamily, member 2; TNF, macrophage derived; TNF, monocyte derived; TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2; Tumor necrosis factor (TNF superfamily member 2); Tumor necrosis factor alpha; Tumor necrosis factor; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor, Membrane Form; Tumor necrosis factor, soluble form |
| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 77-233aa |
| Complete Sequence | M+VRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
| Mol. Weight | 17.5 kDa |
| Protein Length | Partial |
| Tag Info |
Tag-Free |
| Form | Lyophilized powder |
| Buffer | Lyophilized from a 0.2 μm filtered 20 mM PB, 10 mM Nacl, pH 7.0 |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 5-10 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function (From Uniprot) |
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective
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| Gene References into Functions |
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| Involvement in disease | Psoriatic arthritis (PSORAS) |
| Subcellular Location | Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted |
| Protein Families | Tumor necrosis factor family |
| Database Links |
HGNC: 11892 OMIM: 191160 KEGG: hsa:7124 STRING: 9606.ENSP00000398698 UniGene: Hs.241570 |
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