NP Antibody

Code CSB-PA323576ZA01ZAB
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Product Details

Full Product Name
Rabbit anti-Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus) NP Polyclonal antibody
Uniprot No.
Target Names
NP
Alternative Names
NPNucleoprotein antibody; Ebola NP antibody; eNP antibody; Nucleocapsid protein antibody; Protein N antibody
Raised in
Rabbit
Species Reactivity
Zaire ebolavirus
Immunogen
Recombinant Zaire ebolavirus NP protein
Immunogen Species
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm. Each NP binds to six nucleotides of viral genomic RNA, three being exposed to the solvant and three hidden into the nucleocapsid. Recruits also host PPP2R5C phosphatase to dephosphorylate VP30 and thereby promote viral transcription. Upon virion assembly and budding, NP binds to VP24 and possibly host STAU1.
Gene References into Functions
  1. The authors found that the recombinant NP helix is associated with non-viral RNA, which is not protected from RNase digestion and that the morphology of the helix changes depending on the environmental salt concentration. PMID: 20164259
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Filoviruses nucleoprotein family
Database Links

KEGG: vg:911830

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