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Bovine Haptoglobin,Hpt/HP ELISA KIT

Citations (3) Protocol
Code CSB-E08585b
Size 96T,5×96T,10×96T
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Product Details

Target Name
haptoglobin
Alternative Names
HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
Abbreviation
HP
Uniprot No.
Q2TBU0
Species
Bos taurus (Bovine)
Sample Types
serum, plasma
Detection Range
7.81 ng/mL-2000 ng/mL
Sensitivity
7.81 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 84  
Range % 80-88  
1:2000 Average % 90  
Range % 85-94  
1:4000 Average % 103  
Range % 99-112  
1:8000 Average % 98  
Range % 94-102  
Recovery
The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2000 0.243 0.252 0.248    
500 0.412 0.436 0.424    
120 0.793 0.754 0.774    
31.25 1.214 1.239 1.227    
7.81 1.795 1.724 1.760    
0 2.405 2.359 2.382    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Researchers can quantify bovine haptoglobin (Hpt/HP) in serum and plasma with the bovine haptoglobin ELISA Kit. This kit offers a detection range of 7.81 ng/mL-2000 ng/mL and a sensitivity of 7.81 ng/mL. It uses a competitive detection mechanism, wherein the haptoglobin in the sample competes with a labeled haptoglobin for binding to a limited number of antibody sites. This competitive binding results in an inverse correlation between the concentration of haptoglobin in the sample and the assay signal, enabling precise quantification. The kit requires 50-100 μL of sample, with an assay time of 1-5 hours, and detection is performed at a wavelength of 450 nm. This kit has been referenced in upwards of three journal articles.

Citations

Related Products

HP Proteins

Customer Reviews and Q&A

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■ Q&A
Q:

a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
The antibody used in the kit is rabbit polyclonal antibody.
The standard is purified crude extract of native samples.

Target Background

Function
(From Uniprot)
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Gene References into Functions
  1. These results indicate that it is possible to monitor the course of pyometra in cows based on the evaluation of the concentration of cytokines and Hp in the serum and uterine washings. PMID: 25704413
  2. These data demonstrate that serum neutrophil haptoglobin-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins. PMID: 25358728
  3. An increase in serum amyloid A or haptoglobin in dairy cows with abomasal displacementis not specific for inflammation or bacterial infection; increased levels of either may indicate the presence of hepatic lipidosis. PMID: 20391639
  4. Haptoglobin concentration may be a useful indicator for cows with postpartum reproductive disorders. PMID: 14960809
  5. Presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A to see the relationships in cow composite and bulk tank milk samples. PMID: 17227596
  6. Bovine granulocytes, isolated from peripheral blood of healthy cattle, contain abundant amounts of haptoglobin within the granules. PMID: 17681384
  7. This study suggests that neutrophils and epithelial cells may play an essential role in elevating milk haptoglobin (Hp) in addition to previous suggestions that Hp may be derived from mammary tissues and circulation. PMID: 19094922

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Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
Tissue Specificity
Expressed by the liver and secreted in plasma.
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