Pig Haptoglobin,Hpt/HP ELISA Kit

Code CSB-E13424p
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q8SPS7
Lead Time 3-5 working days
Abbreviation HP
Target Name haptoglobin
Alias BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin, alpha polypeptide|haptoglobin, beta polypeptide
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 μg/mL-100 μg/mL
Sensitivity 0.39 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn s disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson s disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene.
HGNC 16937
RGD 708572
MGI 1929501
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 93  
Range % 89-98  
1:40 Average % 98  
Range % 93-103  
1:80 Average % 95  
Range % 89-98  
1:160 Average % 94  
Range % 87-98  
Recovery
The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 94-102  
EDTA plasma (n=4) 87 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ug/ml OD1 OD2 Average Corrected  
100 1.812 1.724 1.768 1.625  
50 1.145 1.212 1.179 1.036  
25 0.715 0.724 0.720 0.577  
12.5 0.451 0.438 0.445 0.302  
6.25 0.312 0.322 0.317 0.174  
3.12 0.255 0.242 0.249 0.106  
1.56 0.196 0.190 0.193 0.050  
0 0.142 0.144 0.143    
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Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

KEGG: ssc:397061

STRING: 9823.ENSSSCP00000002967

UniGene: Ssc.37

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