Pig Haptoglobin,Hpt/HP ELISA Kit

Code CSB-E13424p
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
haptoglobin
Alternative Names
HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
Abbreviation
HP
Uniprot No.
Species
Sus scrofa (Pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 μg/mL-100 μg/mL
Sensitivity
0.39 μg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 93  
Range % 89-98  
1:40 Average % 98  
Range % 93-103  
1:80 Average % 95  
Range % 89-98  
1:160 Average % 94  
Range % 87-98  
Recovery
The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 94-102  
EDTA plasma (n=4) 87 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ug/ml OD1 OD2 Average Corrected  
100 1.812 1.724 1.768 1.625  
50 1.145 1.212 1.179 1.036  
25 0.715 0.724 0.720 0.577  
12.5 0.451 0.438 0.445 0.302  
6.25 0.312 0.322 0.317 0.174  
3.12 0.255 0.242 0.249 0.106  
1.56 0.196 0.190 0.193 0.050  
0 0.142 0.144 0.143    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Pig Haptoglobin (HP) ELISA Kit was designed for the quantitative measurement of Pig Haptoglobin (HP) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 μg/mL-100 μg/mL and the sensitivity is 0.39 μg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Gene References into Functions
  1. crystal structure of the dimeric porcine haptoglobin-haemoglobin complex determined at 2.9 A resolution PMID: 22922649
  2. This protein is elevated in porcine postweaning multisystemic wasting syndrome. PMID: 15210076
  3. haptoglobin has a role in oxidative status in piglets undergoing weaning-induced stress PMID: 16438801
  4. This study suggested closer links between the release of haptoglobin, Pig-MAP and monocytes compared to the release of AGP, SAA and transthyretin. PMID: 17629575
  5. The haptoglobin was down-regulated with SNPs PMID: 18777092
  6. Presence of haptoglobin alpha 1S subunit is a reponse to respiratory syndrome virus infection. PMID: 18931071

Show More

Hide All

Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Tissue Specificity
Expressed by the liver and secreted in plasma.
Database Links

KEGG: ssc:397061

STRING: 9823.ENSSSCP00000002967

UniGene: Ssc.37

CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1