Pig Haptoglobin,Hpt/HP ELISA Kit

Instructions
Code CSB-E13424p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name haptoglobin
Alternative Names HP ELISA Kit; Haptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
Abbreviation HP
Uniprot No. Q8SPS7
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 μg/mL-100 μg/mL
Sensitivity 0.39 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig Hpt/HP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 93  
Range % 89-98  
1:40 Average % 98  
Range % 93-103  
1:80 Average % 95  
Range % 89-98  
1:160 Average % 94  
Range % 87-98  
Recovery
The recovery of pig Hpt/HP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 98 94-102  
EDTA plasma (n=4) 87 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ug/ml OD1 OD2 Average Corrected  
100 1.812 1.724 1.768 1.625  
50 1.145 1.212 1.179 1.036  
25 0.715 0.724 0.720 0.577  
12.5 0.451 0.438 0.445 0.302  
6.25 0.312 0.322 0.317 0.174  
3.12 0.255 0.242 0.249 0.106  
1.56 0.196 0.190 0.193 0.050  
0 0.142 0.144 0.143    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway (By similarity).
Gene References into Functions
  1. crystal structure of the dimeric porcine haptoglobin-haemoglobin complex determined at 2.9 A resolution PMID: 22922649
  2. This protein is elevated in porcine postweaning multisystemic wasting syndrome. PMID: 15210076
  3. haptoglobin has a role in oxidative status in piglets undergoing weaning-induced stress PMID: 16438801
  4. This study suggested closer links between the release of haptoglobin, Pig-MAP and monocytes compared to the release of AGP, SAA and transthyretin. PMID: 17629575
  5. The haptoglobin was down-regulated with SNPs PMID: 18777092
  6. Presence of haptoglobin alpha 1S subunit is a reponse to respiratory syndrome virus infection. PMID: 18931071

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Subcellular Location Secreted
Protein Families Peptidase S1 family
Tissue Specificity Expressed by the liver and secreted in plasma.
Database Links

KEGG: ssc:397061

STRING: 9823.ENSSSCP00000002967

UniGene: Ssc.37

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