Human Gastrin ELISA Kit

Instructions
Code CSB-E09166h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gastrin
Alternative Names Component II ELISA Kit; G14 ELISA Kit; G17 ELISA Kit; G34 ELISA Kit; G52 ELISA Kit; G6 ELISA Kit; GAS ELISA Kit; Gast ELISA Kit; GAST_HUMAN ELISA Kit; Gastrin ELISA Kit; Gastrin component I ELISA Kit; Gastrin component II ELISA Kit; Gastrin component III ELISA Kit; Gastrin precursor ELISA Kit; Gastrin-14 ELISA Kit; Gastrin-17 ELISA Kit; Gastrin-34 ELISA Kit; Gastrin-52 ELISA Kit; Gastrin-6 ELISA Kit; Gastrin-71 ELISA Kit
Abbreviation GAST
Uniprot No. P01350
Species Homo sapiens (Human)
Sample Types serum, plasma, urine
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %84-90
1:2Average %83
Range %80-86
1:4Average %95
Range %91-99
1:8Average %84
Range %80-88
Recovery
The recovery of human gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9683-89
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.514 2.569 2.542 2.368
10001.954 1.901 1.928 1.754
5001.483 1.472 1.478 1.304
2501.005 1.034 1.020 0.846
1250.568 0.578 0.573 0.399
62.50.382 0.398 0.390 0.216
31.250.247 0.261 0.254 0.080
00.173 0.175 0.174
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Gene References into Functions
  1. We concluded that low serum gastrin is related to increased risk of ER(+) BC development. The results also established that CCKBR/ERK/P65 signaling function is generally tumor suppressive in ER(+) BC, indicating therapies should focus on restoring, not inhibiting, CCKBR/ERK/P65 pathway activity. PMID: 30115027
  2. Study reveals that Galphas binds to RGS1 and plays a necessary role during RGS1mediated promotion of melanoma proliferation and migration. PMID: 29620236
  3. Gastrin and BCL2 apoptosis regulator (Bcl2) are highly expressed in gastric cancer tissues, and they are correlated with the clinicopathologic features. PMID: 29268861
  4. Everolimus down-regulates the systemic levels of gastrin and glucagon in patients with pancreatic neuroendocrine tumors. PMID: 28609362
  5. we suggest that gastrin contributes to the emergence of MDR of SGC7901 cells via the degradation of p27Kip1 PMID: 28498440
  6. Results demonstrate that gastrin increases the expression of the autophagy markers MAP1LC3B-II and SQSTM1 in gastric adenocarcinoma cells. Gastrin induces autophagy via activation of the STK11-PRKAA2-ULK1 and that this signaling pathway is involved in increased migration and cell survival. PMID: 28109268
  7. PTHLH is a gastrin-regulated growth factor that might contribute to gastric epithelial cell homeostasis. PMID: 28408643
  8. Gastrin is expressed in both insulin(+) and somatostatin(+) cells in adults with type 2 diabetes. PMID: 27864307
  9. Gastrin induced ABCG2 expression by activating NF-kappaB and thereby modulated the side population proportion, tumor cell metastatic potential and invasion activity in pancreatic cancer. PMID: 27264047
  10. CTGF is overexpressed in gastric cancer and adjacent tissue compared to normal gastric tissue. Gastrin induces expression of CTGF in gastric epithelial cells. PMID: 27179776
  11. Data show that 6 of 20 primary pancreatic neuroendocrine tumors (PNETs) had positive staining for multiple hormones gastrin, insulin, glucagon, and somatostatin, and positive expression of 1 or more hormones was found in 9 of 12 nonfunctioning PNETs [NF-PNETs]) patients. PMID: 26684862
  12. Gastrin role in gastric epithelial homeostasis and gastric carcinogenesis PMID: 25752269
  13. Results suggest a role for Toll-like receptor 4 in gastric acid regulation and that the Toll-like receptor 4 (TLR4) +896 and +1196 wild type homozygozity increases peptic ulcer risk via gastrin secretion. PMID: 26161647
  14. Serum pepsinogens I (PGI) and II (PGII), gastrin 17 (G-17), and antibodies against whole H. pylori, or cytotoxin-associated gene A (CagA) antigen among 309 consecutive patients, were measured.[Pepsinogen I, Gastrin 17] PMID: 22066020
  15. treatment with gastrin, a CCK2R agonist, stimulated the secretion of GLP-1, and that this effect was likely due to increased expression of proglucagon and PCSK1 (also known as prohormone convertase 3 (PC3 gene)). PMID: 25601282
  16. Gastrin stimulates MMP-1 expression in gastric epithelial cells and stimulates cell migration. PMID: 25977510
  17. Progastrin is a new pro-angiogenic factor in colorectal cancer and an attractive therapeutic target. PMID: 25109333
  18. Small (PMID: 25993755
  19. Gastrin values were higher in erosive esophagitis patients taking proton pump inhibitors compared to controls. There was no correlation between gastrin levels and treatment duration. Female patients had significantly higher gastrin values than males. PMID: 24210828
  20. In autoimmune gastritis, the authors were able to find evidence for a new gastrin-mediated mechanism for ghrelin suppression as a gastro-protective marker. PMID: 24388886
  21. NR4A2 is regulated by gastrin and influences cellular responses of gastric adenocarcinoma cells. PMID: 24086717
  22. A novel signal pathway involved in gastrin-stimulated AE2 expression mediated by EGR1 in gastric cancer cells. PMID: 23121767
  23. Investigation of the healthy human pylorus shows characteristic distribution of cells that react with antisera to gastrin and somatostatin. PMID: 22532184
  24. hypoxia up-regulates the gastrin gene in AGS cells by HIF-independent mechanisms PMID: 22593272
  25. The low serum levels of pepsinogen and G17 are predictive of extensive gastric atrophy with high-risk of early gastric cancer. PMID: 21222340
  26. progastrin predicts colonic neoplasia outcome in patients with hyperplastic colonic polyps PMID: 22366915
  27. analysis of serum gastrin concentrations in children with primary gastroesophageal reflux and gastroesophageal reflux secondary to cow's milk allergy PMID: 22112435
  28. gastrin-17 has a role in antral atrophy PMID: 22037174
  29. Helicobacter Pylori CagA protein activates the gastrin promoter to up-regulate gastrin mRNA expression through the JAK2-signaling pathway in gastric cancer cells. PMID: 21509655
  30. Data show that alphav integrin is a new gastrin target in pancreatic cancer models and contributes to gastrin effects on cell adhesion. PMID: 22110279
  31. Data suggest that elevated plasma gastrin and suppression of fasting ghrelin in patients with gastric cancer indicate a close relationship between these two hormones in gastric carcinogenesis. PMID: 21274374
  32. CagA may activate the gastrin promoter and up-regulate the expression of gastrin. PMID: 21029692
  33. Pancreatic cancer cells produce CCK and gastrin; however, the autocrine production of gastrin is more important for stimulating tumor growth. PMID: 21186400
  34. Initiation of antral gastric cancer is associated with progressive epigenetic silencing of TFF1, which can be suppressed by the hormone gastrin. PMID: 21111741
  35. No correlation was found between gastrin-17 levels and the degree of corpus atrophy in gastroesophageal reflux patients. PMID: 21175799
  36. iron binding to progastrin may regulate processing and secretion in vivo, and regulation may be particularly important in diseases with altered iron homeostasis. PMID: 21195058
  37. a positive-feedback loop whereby gastrin, acting via the CCK2 receptor, increases its own expression PMID: 20932834
  38. Data show an increasing tendency in the expressions of GAS and SS in children with chronic gastritis and duodenal ulcer. PMID: 17229384
  39. Progastrin/glycine extended gastrin stimulation of colorectal mucosa proliferation is dependent on the presence of ferric ions. PMID: 20395538
  40. In patients with autoimmune Addison's disease hyperchromograninemia and hypergastrinemia occur with a prevalence of 27.5 and 22.5%, respectively. PMID: 19794299
  41. Preprandial oral dose of sitagliptin does not affect circulating gastrin, but it increased gastrin levels after meal loading. PMID: 20145424
  42. The mRNA and protein expressions of GAS in well and moderately differentiated colorectal cancers were significantly lower than those in poorly differentiated cancers. PMID: 19031134
  43. there was a significant decrease in gastrin plasma concentrations in patients with diabetic autonomic neuropathy PMID: 11710799
  44. present in human colorectal adenocarcinomas and liver metatases PMID: 12174892
  45. Gastrin induces proliferation in Barrett's metaplasia through activation of the CCK2 receptor. PMID: 12612900
  46. Serum progastrin and gastrin levels are significantly higher in GC patients than in matched controls, confirming that both gastrins may be implicated in gastric carcinogenesis. PMID: 14627349
  47. the dibasic processing sites constitute the most important sorting domain of progastrin PMID: 14660571
  48. Differentially expressed in gastric cancer and intestinal metaplasia. PMID: 14719064
  49. Human gastric carcinomas and gastric cancer cell line SGC-7901 cells coexpress CCK-B receptor and gastrin mRNA. PMID: 15040018
  50. gastric hormone gastrin stimulates gastric acid secretion and epithelial cell proliferation--REVIEW PMID: 15533775

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Subcellular Location Secreted
Protein Families Gastrin/cholecystokinin family
Database Links

HGNC: 4164

OMIM: 137250

KEGG: hsa:2520

STRING: 9606.ENSP00000331358

UniGene: Hs.2681

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