Mouse Gastrin ELISA Kit

Instructions
Code CSB-E12924m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name gastrin
Alternative Names Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Gastrin-71 ELISA Kit; G71); Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
Abbreviation GAST
Uniprot No. P48757
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 pg/mL-80 pg/mL
Sensitivity 0.31 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-99  
1:2 Average % 98  
Range % 92-105  
1:4 Average % 94  
Range % 88-97  
1:8 Average % 88  
Range % 83-95  
Recovery
The recovery of mouse Gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-99  
EDTA plasma (n=4) 98 95-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
80 2.785 2.650 2.718 2.555  
40 2.384 2.375 2.380 2.217  
20 1.689 1.752 1.721 1.558  
10 0.974 1.030 1.002 0.839  
5 0.575 0.532 0.554 0.391  
2.5 0.362 0.357 0.360 0.197  
1.25 0.247 0.258 0.253 0.090  
0 0.161 0.165 0.163    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Gene References into Functions
  1. Gastrin induces nuclear export and proteasome degradation of menin in duodenal glial cells. PMID: 28859856
  2. PTHLH is a gastrin-regulated growth factor that might contribute to gastric epithelial cell homeostasis. PMID: 28408643
  3. Renal-selective silencing of Gast by the renal subcapsular injection of Gast siRNA in BALB/c mice decreases renal dopamine production and increases blood pressure. PMID: 27780818
  4. Gastrin is expressed in insulin secreting cells of adult diabetic mice. PMID: 27864307
  5. increased circulating concentrations of progastrin provide a physiological advantage against systemic hypoxia in mice PMID: 25394662
  6. Ihh signaling mediates gastrin-induced proliferation of epithelial cells in stomachs of adult mice. PMID: 24859162
  7. Data indicate that epithelial Gli2 expression was sufficient to stimulate Il-1beta expression, repress Gast gene expression and increase proliferation, leading to antral hyperplasia. PMID: 23110168
  8. gastrins play an important direct role, unrelated to their ability to stimulate acid secretion, in hematopoiesis under conditions of iron deficiency. PMID: 21652729
  9. Initiation of antral gastric cancer is associated with progressive epigenetic silencing of TFF1, which can be suppressed by the hormone gastrin. PMID: 21111741
  10. Regulation of mouse gastrin gene in vivo. Mechanisms involved in transcriptional control of the gastrin gene. PMID: 21051525
  11. RegI and gastrin may synergistically regulate gastric mucosal proliferation during certain pathological settings like wound healing PMID: 19969026
  12. To examine the importance of gastrin or its receptors during development in general and for gastric physiology specifically both have been knocked out. PMID: 11713979
  13. stimulates the growth of gastric pit cell precursors by inducing its own receptors. PMID: 11804858
  14. Endocrine gastrin may have a role in the colonic adenoma carcinoma sequence. Hyper-gastrinaemia may promote proliferation of human colonic adenomas that express CCK-2 receptor isoforms. PMID: 12189558
  15. Parietal cells were found to be significantly smaller in the mutant cell preparations, suggesting that gastrin stimulation modulates parietal cell morphology. PMID: 12388185
  16. essential physiologic role for gastrin in glucose homeostasis; however, the gastrin gene is not essential for murine islet development or the adaptive islet proliferative response to beta-cell injury. PMID: 14517799
  17. gastrin promoter is activated by transforming growth factor-beta/Smad and Wnt signaling pathways PMID: 15292219
  18. gastrin expression, possibly contributing to neoplastic progression, is induced by synergistic Wnt and Ras signaling pathway PMID: 16139800
  19. The aim of this study was to evaluate changes in the acid-secreting parietal cell in gastrin-deficient (GAS-KO) mice. PMID: 16278279
  20. Hypergastrinemia increases regeneration of intestinal epithelia following diverse forms of injury. PMID: 16618411
  21. gastrin can induce apoptosis in gastric epithelial cells and contribute to the development of gastric carcinogenesis PMID: 16894354
  22. Gastrin concentrations are increased in the plasma and gastric mucosa of Hfe(-/-) mice, and in the sera of humans with HFE-related hemochromatosis. PMID: 17064691
  23. Gastrin regulates TFF2 transcription through a GC-rich DNA-binding site and a protein kinase dependent pathway. PMID: 17332476
  24. Identification of ezrin as a target of gastrin in immature mouse gastric parietal cells. PMID: 18567601
  25. Results suggest that RNAi targeting of gastrin could serve as an effective treatment for pancreatic cancer. PMID: 19465883

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Subcellular Location Secreted
Protein Families Gastrin/cholecystokinin family
Tissue Specificity Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.
Database Links

KEGG: mmu:14459

STRING: 10090.ENSMUSP00000017309

UniGene: Mm.4767

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