Mouse Gastrin ELISA Kit

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Target Background

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Product Details

Description

This Mouse GAST ELISA Kit was designed for the quantitative measurement of Mouse GAST protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.25 pg/mL-80 pg/mL and the sensitivity is 0.31 pg/mL .

Target Name

gastrin

Alternative Names

Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Gastrin-71 ELISA Kit; G71); Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit

Abbreviation

GAST

Uniprot No.

P48757

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

1.25 pg/mL-80 pg/mL

Sensitivity

0.31 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-99
1:2	Average %	98
	Range %	92-105
1:4	Average %	94
	Range %	88-97
1:8	Average %	88
	Range %	83-95

Recovery

The recovery of mouse Gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-99
EDTA plasma (n=4)	98	95-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
80	2.785	2.650	2.718	2.555
40	2.384	2.375	2.380	2.217
20	1.689	1.752	1.721	1.558
10	0.974	1.030	1.002	0.839
5	0.575	0.532	0.554	0.391
2.5	0.362	0.357	0.360	0.197
1.25	0.247	0.258	0.253	0.090
0	0.161	0.165	0.163

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Gene References into Functions	Gastrin induces nuclear export and proteasome degradation of menin in duodenal glial cells. PMID: 28859856 PTHLH is a gastrin-regulated growth factor that might contribute to gastric epithelial cell homeostasis. PMID: 28408643 Renal-selective silencing of Gast by the renal subcapsular injection of Gast siRNA in BALB/c mice decreases renal dopamine production and increases blood pressure. PMID: 27780818 Gastrin is expressed in insulin secreting cells of adult diabetic mice. PMID: 27864307 increased circulating concentrations of progastrin provide a physiological advantage against systemic hypoxia in mice PMID: 25394662 Ihh signaling mediates gastrin-induced proliferation of epithelial cells in stomachs of adult mice. PMID: 24859162 Data indicate that epithelial Gli2 expression was sufficient to stimulate Il-1beta expression, repress Gast gene expression and increase proliferation, leading to antral hyperplasia. PMID: 23110168 gastrins play an important direct role, unrelated to their ability to stimulate acid secretion, in hematopoiesis under conditions of iron deficiency. PMID: 21652729 Initiation of antral gastric cancer is associated with progressive epigenetic silencing of TFF1, which can be suppressed by the hormone gastrin. PMID: 21111741 Regulation of mouse gastrin gene in vivo. Mechanisms involved in transcriptional control of the gastrin gene. PMID: 21051525 RegI and gastrin may synergistically regulate gastric mucosal proliferation during certain pathological settings like wound healing PMID: 19969026 To examine the importance of gastrin or its receptors during development in general and for gastric physiology specifically both have been knocked out. PMID: 11713979 stimulates the growth of gastric pit cell precursors by inducing its own receptors. PMID: 11804858 Endocrine gastrin may have a role in the colonic adenoma carcinoma sequence. Hyper-gastrinaemia may promote proliferation of human colonic adenomas that express CCK-2 receptor isoforms. PMID: 12189558 Parietal cells were found to be significantly smaller in the mutant cell preparations, suggesting that gastrin stimulation modulates parietal cell morphology. PMID: 12388185 essential physiologic role for gastrin in glucose homeostasis; however, the gastrin gene is not essential for murine islet development or the adaptive islet proliferative response to beta-cell injury. PMID: 14517799 gastrin promoter is activated by transforming growth factor-beta/Smad and Wnt signaling pathways PMID: 15292219 gastrin expression, possibly contributing to neoplastic progression, is induced by synergistic Wnt and Ras signaling pathway PMID: 16139800 The aim of this study was to evaluate changes in the acid-secreting parietal cell in gastrin-deficient (GAS-KO) mice. PMID: 16278279 Hypergastrinemia increases regeneration of intestinal epithelia following diverse forms of injury. PMID: 16618411 gastrin can induce apoptosis in gastric epithelial cells and contribute to the development of gastric carcinogenesis PMID: 16894354 Gastrin concentrations are increased in the plasma and gastric mucosa of Hfe(-/-) mice, and in the sera of humans with HFE-related hemochromatosis. PMID: 17064691 Gastrin regulates TFF2 transcription through a GC-rich DNA-binding site and a protein kinase dependent pathway. PMID: 17332476 Identification of ezrin as a target of gastrin in immature mouse gastric parietal cells. PMID: 18567601 Results suggest that RNAi targeting of gastrin could serve as an effective treatment for pancreatic cancer. PMID: 19465883 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Gastrin/cholecystokinin family
Tissue Specificity	Abundantly expressed in the stomach and duodenum. Low levels in brain, ovary and pancreas.
Database Links	KEGG: mmu:14459 STRING: 10090.ENSMUSP00000017309 UniGene: Mm.4767

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Code	CSB-E12924m
Size	96T,5×96T,10×96T How to order?
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.