Rat Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit

Code CSB-E07917r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name plasminogen activator, tissue
Alternative Names Plat ELISA Kit; Tissue-type plasminogen activator ELISA Kit; t-PA ELISA Kit; t-plasminogen activator ELISA Kit; tPA ELISA Kit; EC 3.4.21.68) [Cleaved into: Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B] ELISA Kit
Abbreviation PLAT
Uniprot No. P19637
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat t-PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %85-97
1:2Average %95
Range %91-99
1:4Average %96
Range %89-103
1:8Average %100
Range %94-105
Recovery
The recovery of rat t-PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10196-106
EDTA plasma (n=4)9490-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
201.946 1.846 1.896 1.753
101.542 1.440 1.491 1.348
51.019 0.989 1.004 0.861
2.50.536 0.529 0.533 0.390
1.250.330 0.320 0.325 0.182
0.6250.278 0.268 0.273 0.130
0.3120.205 0.201 0.203 0.060
00.144 0.142 0.143  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

Is the kit of tPA(tissue plasminogen activator) cab be used for kidney rat lysat?
The research is about preeclampsia and damage to the kidney in pregnancy. We have a material that raises the level of tPA. tPA is anti coagulation protien that we think is involved in antiapoptosis.
We injected this material to pregnancy rats with preeclampsia, and we took out the kidneys. Now we want to test the level of tPa and its inhibitor PAI1, in this kidneys.
Please email me the product number?

A:
Thanks for your inquiry.
We have this kit for you: CSB-E07917r,Rat Tissue-type Plasiminogen Actilyse,t-PA ELISA Kit.
Additionally, if you want to test PAI1,we have this kit available for you: CSB-E07948r,Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit.
I will send you the kit manuals by email.
Regarding to the 2 cases you mentioned, sorry we did not validate before.We suggest you do a pretest first.
If further questions,pls let us know.

Target Background

Function
(From Uniprot)
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy.
Gene References into Functions
  1. t-PA expressed in ECs may help to form IT of the DA via activation of MMP-2 and disruption of IEL PMID: 29304073
  2. LRP1 assembles unique co-receptor systems to initiate cell signaling in response to tissue-type plasminogen activator and myelin-associated glycoprotein. PMID: 24129569
  3. Regulation of PAI-1 activity in astrocytes by valproic acid may affect both physiological and pathological processes in the brain by upregulating tPA activity. PMID: 23378038
  4. Together, because CPAI blocks both proteolytic and nonproteolytic tPA neurotoxicity, it is a promising therapeutics of neonatal HI injury either with or without infection. PMID: 22556277
  5. Designed t-PA mutants with molecular properties that, in contrast to t-PA, do not induce neurotoxicity. PMID: 23301636
  6. Tissue plasminogen activator prevents mortality from sulfur mustard analog-induced airway obstruction. PMID: 23258228
  7. NR2B as a novel proteolytic substrate of tPA, where tPA may directly interact with NR2B subunits leading to a change in pharmacological properties of NR2B-containing NMDA receptors. PMID: 22610100
  8. mRNA, protein and activity of matrix metalloproteinase (MMP)-2 and MMP-9 and mRNA of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor 1 (PAI-1) were quantified in abdominal aorta (AA) and femoral artery (FA). PMID: 22071631
  9. The induction of tPA by human marrow stromal cells after traumatic brain injury may be one of the mechanisms involved in promoting functional improvement after traumatic brain injury. PMID: 21355820
  10. Hydrocortisone up-regulates PAI-1 expression in a tissue-specific manner along with down-regulation of tPA activity in both normal and inflammatory conditions. PMID: 21412817
  11. PACAP stimulates the release of tPA which promotes cerebellar granule cell survival by a mechanism dependent upon its proteolytic activity. PMID: 21919910
  12. Massive up-regulation of plasminogen activator inhibitor-1 (PAI-1) in astrocytes during subchronic and chronic inflammatory conditions leads to decreased tPA activity in the later stages of reperfusion injury. PMID: 21193004
  13. Erigeron breviscapus injection can inhibit the expression of TNF-alpha and PAI-1, and increase the expression of tPA in rats with acute myocardial infarction. PMID: 21355198
  14. Investigated the brain-protective molecular mechanism of TPA in rats. TPA derivates were intracerebroventricularly administered at 15 min before, and 15, 90, 120 min after middle cerebral artery occlusion. PMID: 20596602
  15. Annexin A2 combined with low-dose tPA improves thrombolytic therapy in a rat model of focal embolic stroke. PMID: 20068577
  16. alpha-synuclein up-regulated MMP-9 and down-regulated tPA activity in rat primary astrocytes and microglia in ERK1/2 dependent fashion; suggests a role of alpha-synuclein via regulation of protease systems through modulation of ERK1/2 activity in brain PMID: 20026244
  17. Both annexin II and TPA were detected on the plasma membrane in a study of the role of proteases in fibronectin matrix remodeling in thyroid epithelial cell monolayer cultures as cells achieve confluence (annexin II)(tissue plasminogen activator) PMID: 11928811
  18. a chemical sympathectomy study of regulation of the vessel wall release of tissue plasminogen activator by sympathetic nervous system neurons PMID: 12192298
  19. Reciprocal actions of neural cell adhesion molecule and tissue plasminogen activator via a Ras-dependent mitogen-activated protein kinase signaling pathway in hippocampal neurons. PMID: 12387826
  20. Enhanced release of enzymatically active tissue plasminogen activator from sympathetic neurons into artery walls and the blood accompanies the changes associated with vascular aging. PMID: 12548713
  21. Low to moderate doses of rt-PA protect the microvascular basal lamina lamina during experimental focal cerebral ischemia, whereas high doses of rt-PA have the opposite effect, probably due to increased coactivation of MMP-2 and MMP-9. PMID: 12783121
  22. after transient focal cerebral ischemia, there are time dependent changes in tPA expression PMID: 14512838
  23. The 600 bp of the tPA promoter upstream of the transcription start site conveys cell specificity to tPA expression and an NF1 site within this region acts as a repressor. PMID: 15044208
  24. tPA activity is rapidly increased in hippocampal neurons after glutamate stimulation, corresponding to an increase in translational activation of mRNA present at the time of stimulation. PMID: 15496678
  25. These results suggest the importance of the t-PA/plasmin cascade during the early pathological stages of delayed neuronal death in the hippocampus following transient forebrain ischemia. PMID: 15882815
  26. Sympathetic nerves are the primary source of stress-induced release of t-PA into and from the densely innervated resistance arteries and arterioles. PMID: 16019605
  27. tPA upregulates brain MMP-9 levels in stroke in vivo PMID: 16051896
  28. tPA is localized in dendrites and terminals of AVP-expressing neurons; tPA is released in an activity-dependent manner, suggesting that matrix-degrading proteases are candidate molecules to be concerned with the structural plasticity in the HNS. PMID: 16150423
  29. tPA acts as a cytokine that binds to the cell membrane receptor LRP-1, induces its tyrosine phosphorylation, and triggers intracellular signal transduction to induce MMP9 gene expression PMID: 16303771
  30. TPA resides in dense-core granules that traffic to postsynaptic dendritic spines and that can remain there for extended periods. Depolarization by potassium elicits exocytotic release of tPA from DCGs in spines that is dependent on calcium signaling. PMID: 16555239
  31. Astrocyte-derived tPA and the proteolytic activity in the dorsal horn may be one of the essential factors involved in pain following root injury. PMID: 17299772
  32. Sertoli cells isolated from rat testes at various ages in vitro secreted tPA in an age-dependent manner. PMID: 17992606
  33. tPA induces LRP-1 tyrosine phosphorylation, which in turn facilitates the LRP-1-mediated recruitment of beta1 integrin and downstream ILK signaling, thereby leading to myofibroblast activation. PMID: 18037995
  34. The critical period of visual cortical plasticity, which occurs in early postnatal life, correlates well with tPA expression in the rat visual cortex. PMID: 18523654
  35. function of the tPA during diapedesis of monocytes through brain endothelial barrier; monocytes induce the release of tPA by brain endothelial cells; tPA and ERK1/2 control the breakdown of the tight junction protein occludin. PMID: 18714030
  36. The increase of tPA in the acute phase after hypoxic-ischemic brain damage may be helpful to clot dissolving, but it induces neuronal apoptosis and aggravates brain injury. PMID: 18947492
  37. FSH-induced ERK1/2 and p38 MAPK activation is capable of regulating tPA production in cultured primary granulosa cells. PMID: 18982462
  38. Our results demonstrated the involvement of tPA in the secondary pathogenesis after spinal cord injury as well as the therapeutic potential of hUCB. PMID: 19152029
  39. t-PA and PAI-1 mRNA were decreased after sympathectomy and remained unchanged after AT1 receptors blockade PMID: 19403340
  40. protein aggregates formed during neuronal injury provide a macromolecular, nonfibrin cofactor that promotes tPA-mediated plasmin formation and subsequent cell breakdown PMID: 19584397

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Subcellular Location Secreted, extracellular space.
Protein Families Peptidase S1 family
Database Links

KEGG: rno:25692

STRING: 10116.ENSRNOP00000025764

UniGene: Rn.107102

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