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Mouse Thyroglobulin,TG ELISA Kit

Citations (4) Protocol
Code CSB-E08241m
Size 96T,5×96T,10×96T
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Product Details

Target Name
thyroglobulin
Alternative Names
Tg ELISA Kit; Tgn ELISA Kit; Thyroglobulin ELISA Kit; Tg ELISA Kit
Abbreviation
TG
Uniprot No.
O08710
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
10 ng/mL-160 ng/mL
Sensitivity
10 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 90    
Range % 85-100    
1:2 Average % 89    
Range % 82-100    
1:4 Average % 93    
Range % 88-100    
1:8 Average % 95    
Range % 89-105    
Recovery
The recovery of mouse TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 92-110    
EDTA plasma (n=4) 95 89-100    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average 　    
160 0.165 0.170 0.168 　    
80 0.276 0.271 0.274 　    
40 0.504 0.499 0.502 　    
20 1.008 1.034 1.021 　    
10 2.055 2.029 2.042 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Thyroglobulin (TG) is a large glycoprotein produced exclusively by thyroid follicular cells and serves as the precursor for thyroid hormone synthesis. This protein functions as a scaffold for the iodination and coupling reactions that generate triiodothyronine (T3) and thyroxine (T4) within thyroid follicles. Circulating thyroglobulin levels are commonly used as a biomarker in thyroid research and clinical diagnostics, particularly for monitoring thyroid function and detecting thyroid-related disorders. Changes in thyroglobulin expression and secretion are linked to various thyroid pathologies. This makes it a valuable target for studying thyroid physiology and disease mechanisms.

The Mouse Thyroglobulin ELISA Kit (CSB-E08241m) provides quantitative measurement of TG in Mus musculus samples. This competitive assay works with serum, plasma, and tissue homogenates. It has a detection range of 10 ng/mL to 160 ng/mL and a sensitivity of 10 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, giving researchers a reliable tool for thyroglobulin quantification in mouse studies.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

This ELISA kit has been used in thyroid-related research to quantify thyroglobulin levels in mouse models. The applications span endocrine studies examining thyroid function and autoimmune conditions affecting thyroid tissue.

• Autoimmune thyroid research: Measuring thyroglobulin antibody markers in serum samples from mouse models of thyroid autoimmunity
• Thyroid tissue analysis: Measuring intrathyroidal thyroglobulin concentrations alongside other thyroid hormones in tissue samples
• Endocrine profiling: Quantifying thyroglobulin as part of comprehensive thyroid function evaluation in experimental studies

Citations

Related Products

Tg Proteins

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Target Background

Function
(From Uniprot)
Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
Gene References into Functions
  1. Findings suggest that coumestrol treatment may have some beneficial actions against thyroid-specific thyroglobulin (Tg) autoantibody production in the development of autoimmune thyroiditis through suppression of Th1 response. PMID: 27384679
  2. these data identify for the first time a Tg T cell epitope as a spontaneous target in ISAT. PMID: 24879795
  3. Final acquisition of secretory competence includes conformational maturation in the interval between linker and hinge segments of region I of thyroglobulin. PMID: 21816825
  4. The results showed that thyroglobulin message or protein is not expressed in placenta, decidua, or ovary in any stages of pregnancy. PMID: 20236260
  5. Findings demonstrate a novel aspect of murine and human thyroid autoimmunity, namely breaking B cell self-tolerance occurs first for Tg and subsequently for TPO. PMID: 20573721
  6. These results indicate that Runx2 deficiency in mice causes decreased Tg expression and a novel type of hypothyroidism. PMID: 20375239
  7. Development of iodine-accelerated autoimmune thyroiditis in NOD.H2h4 mice is not associated with enhanced iodine organification or differential B- or T-cell responses to iodinated determinants in Tg. PMID: 20687399
  8. specific properties of distinct Tg ChEL mutants may result in distinct patterns of Tg monomer folding PMID: 20353937
  9. Five novel autoimmune thyroiditis-causing T cell epitopes of thyroglobulin (Tgn) have been delineated and mapped with an algorithm that predicts Tgn immunopathogenic peptides containing I-A(k)-binding motifs. PMID: 12391254
  10. iodotyrosyl formation in normal thyroglobulin confers pathogenic potential to certain peptides that may otherwise remain innocuous and undetectable by conventional mapping methods PMID: 16547286
  11. Tg could initiate glomerular injury by reproducing the actions of TGF-beta1 in the mesangial cell. PMID: 17495424
  12. Data indicate that the R19K mutation induces local misfolding in the amino-terminal domain of Tg that has global effects on Tg transport and thyroid hormonogenesis. PMID: 17916655
  13. Together these results suggest that a unique low molecular weight variant of Tg is expressed in the kidney, where it could serve both physiological and pathological roles, including that of an autoantigen. PMID: 19716808

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Involvement in disease
Defects in Tg are the cause of some forms of goiter. Goiter is an enlargement of the thyroid gland. The variant Pro-2283 exhibits a defect in exit from the endoplasmic reticulum.
Subcellular Location
Secreted.
Protein Families
Type-B carboxylesterase/lipase family
Tissue Specificity
Specifically expressed in the thyroid gland.
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