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| Code | CSB-E09613h |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of human ADA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 84-93 | |||||
| The recovery of human ADA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 84 | 80-88 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E09613h is a sandwich ELISA kit developed to measure concentrations of human adenosine deaminase (ADA) in serum, urine, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADA in samples. The color intensity is measured at 450 nm via a microplate reader.
ADA is a central enzyme that mediates the conversion of adenosine into inosine, and deoxyadenosine into deoxyinosine in the purine catabolic pathway, which is important for many biological processes, including energy transfer, extra and intracellular signaling, and DNA formation. As a metabolic enzyme, ADA is ubiquitously expressed in various cells or tissues, including the lymphatic system. ADA is necessary for the proliferation and differentiation of T lymphocytes, and the maturation and function of monocytes and macrophages. ADA deficiency leads to cellular and humoral immunodeficiency, which manifests as severe combined immunodeficiency disease.
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